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AB51285

Recombinant Human SET7 protein

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(1 Publication)

Recombinant Human SET7 protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

KIAA1717, KMT7, SET7, SET9, SETD7, Histone-lysine N-methyltransferase SETD7, Histone H3-K4 methyltransferase SETD7, Lysine N-methyltransferase 7, SET domain-containing protein 7, SET7/9, H3-K4-HMTase SETD7

2 Images
Western blot - Recombinant Human SET7 protein (AB51285)
  • WB

Unknown

Western blot - Recombinant Human SET7 protein (AB51285)

All lanes:

Anti-SETD7 antibody - C-terminal (<a href='/en-us/products/unavailable/set7-antibody-c-terminal-ab71214'>ab71214</a>) at 1/100 dilution

All lanes:

Western blot - Recombinant Human SET7 protein (ab51285) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 10s

SDS-PAGE - Recombinant Human SET7 protein (AB51285)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SET7 protein (AB51285)

ab51285 run on a 10% SDS-PAGE gel with molecular weight markers.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

Q8WTS6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.79% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab51285 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/set7-antibody-c-terminal-ab71214'>ab71214</a>.</p>" } } }
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Sequence info

[{"sequence":"MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK","proteinLength":"Full Length","predictedMolecularWeight":"40.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8WTS6","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SET7 also known as SETD7 or KMT7 is a lysine methyltransferase that adds methyl groups to lysine residues on target proteins influencing their function and stability. This protein has a molecular mass of approximately 55 kDa. SET7 is expressed in a variety of tissues including the heart liver and pancreas suggesting its versatile role across different biological systems.
Biological function summary

SET7 contributes to gene regulation by methylating histone H3 at lysine 4 (H3K4) a mark generally associated with transcriptional activation. It also methylates non-histone proteins such as p53 TAF10 and STAT3 affecting their activity and interactions. SET7 operates distinctively and does not need to be part of any complex to fulfill its function effectively in diverse cellular processes.

Pathways

SET7 modulates transcriptional pathways such as the p53 signaling pathway and the NF-kB pathway. It interacts with the p53 protein affecting cell cycle regulation and the response to DNA damage. The modification of NF-kB by SET7 can influence inflammation and immune responses. Through these pathways SET7 plays a role in maintaining cellular homeostasis and response to stress.

SET7 is involved in conditions like cancer and cardiovascular diseases. Its methylation of p53 links it to tumor suppression activities while its impact on NF-kB relates to inflammatory responses. Dysregulation of SET7 activity can lead to abnormal cell proliferation and inflammation pointing to its potential as a target for therapeutic intervention.
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Specifications

Form

Liquid

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General info

Function

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed : 11779497, PubMed : 11850410, PubMed : 12540855, PubMed : 12588998, PubMed : 16141209). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 12540855, PubMed : 12588998, PubMed : 16141209). Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed : 12588998, PubMed : 16141209). Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed : 16141209). Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins (PubMed : 15099517, PubMed : 15525938, PubMed : 16415881, PubMed : 35210392). Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II (PubMed : 15099517, PubMed : 16415881). Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation (PubMed : 15525938, PubMed : 16415881, PubMed : 17108971). Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS (By similarity).

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET7 subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed : 11779497, PubMed : 11850410, PubMed : 12540855, PubMed : 12588998, PubMed : 16141209). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 12540855, PubMed : 12588998, PubMed : 16141209). Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed : 12588998, PubMed : 16141209). Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed : 16141209). Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins (PubMed : 15099517, PubMed : 15525938, PubMed : 16415881, PubMed : 35210392). Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II (PubMed : 15099517, PubMed : 16415881). Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation (PubMed : 15525938, PubMed : 16415881, PubMed : 17108971). Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS (By similarity).
See full target information SETD7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eade4186 PubMed37235656

2023

PD-L1 methylation restricts PD-L1/PD-1 interactions to control cancer immune surveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Changsheng Huang,Shengxiang Ren,Yaqi Chen,Anyi Liu,Qi Wu,Tao Jiang,Panjing Lv,Da Song,Fuqing Hu,Jingqing Lan,Li Sun,Xue Zheng,Xuelai Luo,Qian Chu,Keyi Jia,Yan Li,Jun Wang,Caicun Zou,Junbo Hu,Guihua Wang
View all publications
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