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Recombinant Human SET7 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.

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Images

SDS-PAGE - Recombinant Human SET7 protein (AB51285), expandable thumbnail
  • Western blot - Recombinant Human SET7 protein (AB51285), expandable thumbnail

Publications

Key facts

Purity
>95% SDS-PAGE
Expression system
Escherichia coli
Tags
Tag free
Applications
SDS-PAGE, WB
Biologically active
No

Amino acid sequence

M D S D D E M V E E A V E G H L D D D G L P H G F C T V T Y S S T D R F E G N F V H G E K N G R G K F F F F D G S T L E G Y Y V D D A L Q G Q G V Y T Y E D G G V L Q G T Y V D G E L N G P A Q E Y D T D G R L I F K G Q Y K D N I R H G V C W I Y Y P D G G S L V G E V N E D G E M T G E K I A Y V Y P D E R T A L Y G K F I D G E M I E G K L A T L M S T E E G R P H F E L M P G N S V Y H F D K S T S S C I S T N A L L P D P Y E S E R V Y V A E S L I S S A G E G L F S K V A V G P N T V M S F Y N G V R I T H Q E V D S R D W A L N G N T L S L D E E T V I D V P E P Y N H V S K Y C A S L G H K A N H S F T P N C I Y D M F V H P R F G P I K C I R T L R A V E A D E E L T V A Y G Y D H S P P G K S G P E A P E W Y Q V E L K A F Q A T Q Q K

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

ab51285 can be used as a WB positive control in conjunction with ab71214.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed:11779497, PubMed:11850410, PubMed:12540855, PubMed:12588998, PubMed:16141209). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:12540855, PubMed:12588998, PubMed:16141209). Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed:12588998, PubMed:16141209). Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed:16141209). Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins (PubMed:15099517, PubMed:15525938, PubMed:16415881, PubMed:35210392). Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II (PubMed:15099517, PubMed:16415881). Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation (PubMed:15525938, PubMed:16415881, PubMed:17108971). Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS (By similarity).

Alternative names

KIAA1717, KMT7, SET7, SET9, SETD7, Histone-lysine N-methyltransferase SETD7, Histone H3-K4 methyltransferase SETD7, Lysine N-methyltransferase 7, SET domain-containing protein 7, SET7/9, H3-K4-HMTase SETD7

Recommended products

Recombinant Human SET7 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.

Key facts

Purity
>95% SDS-PAGE
Expression system
Escherichia coli
Applications
SDS-PAGE, WB
Accession
Q8WTS6-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 7.5
Constituents: 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.79% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Sequence info

Amino acid sequence

M D S D D E M V E E A V E G H L D D D G L P H G F C T V T Y S S T D R F E G N F V H G E K N G R G K F F F F D G S T L E G Y Y V D D A L Q G Q G V Y T Y E D G G V L Q G T Y V D G E L N G P A Q E Y D T D G R L I F K G Q Y K D N I R H G V C W I Y Y P D G G S L V G E V N E D G E M T G E K I A Y V Y P D E R T A L Y G K F I D G E M I E G K L A T L M S T E E G R P H F E L M P G N S V Y H F D K S T S S C I S T N A L L P D P Y E S E R V Y V A E S L I S S A G E G L F S K V A V G P N T V M S F Y N G V R I T H Q E V D S R D W A L N G N T L S L D E E T V I D V P E P Y N H V S K Y C A S L G H K A N H S F T P N C I Y D M F V H P R F G P I K C I R T L R A V E A D E E L T V A Y G Y D H S P P G K S G P E A P E W Y Q V E L K A F Q A T Q Q K
Accession
Q8WTS6
Protein length
Full Length
Predicted molecular weight
40.7 kDa
Nature
Recombinant

Specifications

Form
Liquid

General info

Function

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed:11779497, PubMed:11850410, PubMed:12540855, PubMed:12588998, PubMed:16141209). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:12540855, PubMed:12588998, PubMed:16141209). Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed:12588998, PubMed:16141209). Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed:16141209). Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins (PubMed:15099517, PubMed:15525938, PubMed:16415881, PubMed:35210392). Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II (PubMed:15099517, PubMed:16415881). Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation (PubMed:15525938, PubMed:16415881, PubMed:17108971). Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS (By similarity).

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET7 subfamily.

Subcellular localisation
Nucleus

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SET7 also known as SETD7 or KMT7 is a lysine methyltransferase that adds methyl groups to lysine residues on target proteins influencing their function and stability. This protein has a molecular mass of approximately 55 kDa. SET7 is expressed in a variety of tissues including the heart liver and pancreas suggesting its versatile role across different biological systems.

Biological function summary

SET7 contributes to gene regulation by methylating histone H3 at lysine 4 (H3K4) a mark generally associated with transcriptional activation. It also methylates non-histone proteins such as p53 TAF10 and STAT3 affecting their activity and interactions. SET7 operates distinctively and does not need to be part of any complex to fulfill its function effectively in diverse cellular processes.

Pathways

SET7 modulates transcriptional pathways such as the p53 signaling pathway and the NF-kB pathway. It interacts with the p53 protein affecting cell cycle regulation and the response to DNA damage. The modification of NF-kB by SET7 can influence inflammation and immune responses. Through these pathways SET7 plays a role in maintaining cellular homeostasis and response to stress.

Associated diseases and disorders

SET7 is involved in conditions like cancer and cardiovascular diseases. Its methylation of p53 links it to tumor suppression activities while its impact on NF-kB relates to inflammatory responses. Dysregulation of SET7 activity can lead to abnormal cell proliferation and inflammation pointing to its potential as a target for therapeutic intervention.

Product promise

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2 product images

  • SDS-PAGE - Recombinant Human SET7 protein (ab51285), expandable thumbnail

    SDS-PAGE - Recombinant Human SET7 protein (ab51285)

    ab51285 run on a 10% SDS-PAGE gel with molecular weight markers.

  • Western blot - Recombinant Human SET7 protein (ab51285), expandable thumbnail

    Western blot - Recombinant Human SET7 protein (ab51285)

    All lanes: Anti-SETD7 antibody - C-terminal (ab71214) at 1/100 dilution

    All lanes: Western blot - Recombinant Human SET7 protein (ab51285) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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