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AB132885

Recombinant Human SETD3 protein

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Recombinant Human SETD3 protein is a Human Full Length protein, in the 1 to 296 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

C14orf154, SETD3, Actin-histidine N-methyltransferase, Protein-L-histidine N-tele-methyltransferase, SET domain-containing protein 3, hSETD3

1 Images
SDS-PAGE - Recombinant Human SETD3 protein (AB132885)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human SETD3 protein (AB132885)

12.5% SDS-PAGE analysis of ab132885 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

Q86TU7

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGKKSRVKTQKSGTGATATVSPKEILNLTSELLQKCSSPAPGPGKEWEEYVQIRTLVEKIRKKQKGLSVTFDGKREDYFPDLMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEELFLWVPRKLLMTVESAKNSVLGPLYSQDRILQAMGNIALAFHLLCERASPNSFWQPYIQTLPSEYDTPLYFEEDEVRYLQSTQAIHDVFSQYKNTARQYAYFYKVIQTHPHANKLPLKDSFTYEDYRWAVSSVMTRQNQIPTEDGSRVTLALIPLWDMCNHTNGLTPEDSFALAVASA","proteinLength":"Full Length","predictedMolecularWeight":"60.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":296,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q86TU7","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SETD3 also known as SET domain-containing protein 3 acts as a protein lysine methyltransferase. It catalyzes the transfer of a methyl group from S-adenosylmethionine to specific lysine residues on target proteins. SETD3 is a protein with a mass of approximately 69 kDa. You can find it expressed in various tissues including muscle tissues and the liver suggesting it has broad physiological roles.
Biological function summary

SETD3 methyltransferase activity influences many cellular processes such as cell proliferation and differentiation muscle cell function and cytoskeleton dynamics. It forms complexes with actin proteins which stabilizes the cytoskeleton. By interacting with actin SETD3 contributes to actin filament dynamics which is important for muscle contraction and cellular movement.

Pathways

The protein SETD3 participates in pathways related to cytoskeletal organization and cellular growth. It plays a role in the regulation of actin dynamics and is involved in the mechanotransduction pathway which connects mechanical signals to cellular responses. SETD3 interacts with proteins such as actin and tropomyosin which are significant in these pathways.

Alterations in SETD3 expression connects to conditions including cancer and myopathy. SETD3 relates to tumors by affecting cell proliferation pathways and studies suggest it can influence tumor growth. In myopathy its interaction with proteins like actin is disrupted affecting muscle function and leading to muscle weakness. These associations make SETD3 an attractive target for research in therapeutics for such diseases.
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Specifications

Form

Liquid

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General info

Function

Protein-histidine N-methyltransferase that specifically mediates 3-methylhistidine (tele-methylhistidine) methylation of actin at 'His-73' (PubMed : 30526847, PubMed : 30626964, PubMed : 30785395, PubMed : 31388018, PubMed : 31993215). Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery (PubMed : 30626964). Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin (PubMed : 30626964, PubMed : 30785395, PubMed : 31388018).

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. SETD3 actin-histidine methyltransferase family.

Post-translational modifications

Phosphorylated by GSK3B, which is required for recognition by the SCF(FBXW7) complex and subsequent degradation.. Ubiquitinated by the SCF(FBXW7) complex following phosphorylation by GSK3B, leading to its degradation by the proteasome.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Protein-histidine N-methyltransferase that specifically mediates 3-methylhistidine (tele-methylhistidine) methylation of actin at 'His-73' (PubMed : 30526847, PubMed : 30626964, PubMed : 30785395, PubMed : 31388018, PubMed : 31993215). Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery (PubMed : 30626964). Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin (PubMed : 30626964, PubMed : 30785395, PubMed : 31388018).
See full target information SETD3
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