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AB268960

Recombinant human SETD6 protein (Active)

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Recombinant human SETD6 protein (Active) is a Human Full Length protein, in the 1 to 473 aa range, expressed in Sf9 cells, with >80%, suitable for SDS-PAGE, FuncS.

View Alternative Names

N-lysine methyltransferase SETD6, SET domain-containing protein 6, SETD6

2 Images
Functional Studies - Recombinant human SETD6 protein (Active) (AB268960)
  • FuncS

Supplier Data

Functional Studies - Recombinant human SETD6 protein (Active) (AB268960)

The specific activity of ab268960 was 380 pmol/min/mg in a methyltransferase assay using histone H2A protein as substrate.

SDS-PAGE - Recombinant human SETD6 protein (Active) (AB268960)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human SETD6 protein (Active) (AB268960)

SDS-PAGE analysis of of ab268960.

Key facts

Purity

>80% SDS-PAGE

Expression system

Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab268960 was 380 pmol/min/mg in a methyltransferase assay using histone H2A protein as substrate.

Accession

Q8TBK2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MATQAKRPRVAGPVDGGDLDPVACFLSWCRRVGLELSPKVSERAGGRRTRGGARAALTSPPAQVAVSRQGTVAGYGMVARESVQAGELLFVVPRAALLSQHTCSIGGLLERERVALQSQSGWVPLLLALLHELQAPASRWRPYFALWPELGRLEHPMFWPEEERRCLLQGTGVPEAVEKDLANIRSEYQSIVLPFMEAHPDLFSLRVRSLELYHQLVALVMAYSFQEPLEEEEDEKEPNSPVMVPAADILNHLANHNANLEYSANCLRMVATQPIPKGHEIFNTYGQMANWQLIHMYGFVEPYPDNTDDTADIQMVTVREAALQGTKTEAERHLVYERWDFLCKLEMVGEEGAFVIGREEVLTEEELTTTLKVLCMPAEEFRELKDQDGGGDDKREEGSLTITNIPKLKASWRQLLQNSVLLTLQTYATDLKTDQGLLSNKEVYAKLSWREQQALQVRYGQKMILHQLLELTS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":473,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Sf9 cells","accessionNumber":"Q8TBK2","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SETD6 also known as SET Domain Containing 6 is a protein methyltransferase involved in the regulation of protein activities through methylation. The protein has a molecular mass of approximately 46 kDa. SETD6 predominantly localizes in the nucleus but can also be found in other cellular compartments depending on cellular conditions. The expression is relatively ubiquitous present in various tissues and cell types indicating its diverse functions in different biological contexts.
Biological function summary

The protein functions as a monomer and it methylates the lysine residues of target proteins which influences their interactions and functionality. Its enzymatic activity plays a critical role in chromatin modification affecting transcriptional regulation by altering the chromatin state. SETD6 modifies several proteins including the nuclear factor NF-κB subunit RELA which impacts inflammatory responses and cell survival. Through its methylation activity SETD6 modulates important cellular processes by changing the structure and function of the chromatin.

Pathways

Scientists place SETD6 in significant cellular pathways such as the NF-κB signaling pathway and chromatin remodeling pathway. Within the NF-κB pathway SETD6 methylation results in repression of NF-κB target genes controlling inflammatory responses. In chromatin remodeling it interacts with proteins such as WDR5 a critical component of the MLL/COMPASS complex to regulate transcription and maintain chromatin structure. These interactions connect SETD6 to wider cellular functions that influence cell fate and response to environmental stressors.

Researchers identify SETD6 dysregulation as a factor in cancer and inflammatory diseases. In cancer abnormal SETD6 activity can lead to altered expression of oncogenes and tumor suppressor genes contributing to tumorigenesis. Connections exist between SETD6 and other cancer-related proteins such as β-catenin influencing pathways linked to cell proliferation and cancer progression. In inflammatory disorders SETD6 modifies RELA impacting the NF-κB pathway and potentially exacerbating inflammatory responses. Understanding SETD6 interactions in these contexts could lead to therapeutic strategies targeting its methylation activity.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Protein-lysine N-methyltransferase. Monomethylates 'Lys-310' of the RELA subunit of NF-kappa-B complex, leading to down-regulation of NF-kappa-B transcription factor activity (PubMed : 21131967, PubMed : 21515635, PubMed : 30189201). Monomethylates 'Lys-8' of H2AZ (H2AZK8me1) (PubMed : 23324626). Required for the maintenance of embryonic stem cell self-renewal (By similarity). Methylates PAK4.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SETD6 subfamily.

Post-translational modifications

Automethylated; Lys-39 and Lys-179 serve as the major automethylation sites.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Protein-lysine N-methyltransferase. Monomethylates 'Lys-310' of the RELA subunit of NF-kappa-B complex, leading to down-regulation of NF-kappa-B transcription factor activity (PubMed : 21131967, PubMed : 21515635, PubMed : 30189201). Monomethylates 'Lys-8' of H2AZ (H2AZK8me1) (PubMed : 23324626). Required for the maintenance of embryonic stem cell self-renewal (By similarity). Methylates PAK4.
See full target information SETD6
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