Recombinant Human SF3B14 protein is a Human Full Length protein, in the 1 to 125 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A M Q A A K R A N I R L P P E V N R I L Y I R N L P Y K I T A E E M Y D I F G K Y G P I R Q I R V G N T P E T R G T A Y V V Y E D I F D A K N A C D H L S G F N V C N R Y L V V L Y Y N A N R A F Q K M D T K K K E E Q L K L L K E K Y G I N T D P P K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:12234937, PubMed:27720643, PubMed:32494006, PubMed:34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed:12234937, PubMed:32494006, PubMed:34822310). Within the 17S U2 SnRNP complex, SF3B6 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed:12234937, PubMed:27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). Within the 17S U2 SnRNP complex, SF3B6 directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing (PubMed:16432215). SF3B6 stabilizes the intron branch site-U2 snRNA duplex, thereby promoting-binding of introns with poor sequence complementarity (PubMed:34822310). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed:15146077, PubMed:33509932).
Alternative names
SAP14, SF3B14, SF3B14A, CGI-110, HSPC175, HT006, SF3B6, Splicing factor 3B subunit 6, Pre-mRNA branch site protein p14, SF3b 14 kDa subunit, SF3B14a
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Recombinant Human SF3B14 protein is a Human Full Length protein, in the 1 to 125 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9Y3B4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0292% EDTA
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A M Q A A K R A N I R L P P E V N R I L Y I R N L P Y K I T A E E M Y D I F G K Y G P I R Q I R V G N T P E T R G T A Y V V Y E D I F D A K N A C D H L S G F N V C N R Y L V V L Y Y N A N R A F Q K M D T K K K E E Q L K L L K E K Y G I N T D P P K
- Accession
- Q9Y3B4
- Protein length
- Full Length
- Predicted molecular weight
- 16.7 kDa
- Amino acids
- 1 to 125
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
General info
- Function
Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:12234937, PubMed:27720643, PubMed:32494006, PubMed:34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed:12234937, PubMed:32494006, PubMed:34822310). Within the 17S U2 SnRNP complex, SF3B6 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed:12234937, PubMed:27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). Within the 17S U2 SnRNP complex, SF3B6 directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing (PubMed:16432215). SF3B6 stabilizes the intron branch site-U2 snRNA duplex, thereby promoting-binding of introns with poor sequence complementarity (PubMed:34822310). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed:15146077, PubMed:33509932).
- Sequence similarities
Belongs to the SF3B6 family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SF3B14 also known as Splicing Factor 3b Subunit 14 is a component of the splicing factor 3b (SF3b) complex. It has a molecular mass of approximately 14 kDa. SF3B14 is prominently expressed in various tissues with a strong presence in the nucleus where it participates in the spliceosomal machinery. Its mechanical role is to bind and stabilize pre-mRNA as a part of the spliceosome which is essential for proper splicing of precursor mRNA into mature mRNA.
- Biological function summary
SF3B14 contributes to the precise assembly of the spliceosome complex being a significant component of the SF3b complex. This complex is part of the U2 small nuclear ribonucleoprotein (snRNP) within the spliceosome. SF3B14 alongside other components of the SF3b complex ensures the accurate recognition and removal of introns during gene expression. By facilitating correct splicing it plays a role in regulating gene expression levels and maintaining cell function.
- Pathways
Spliceosomes and their components including SF3B14 play a role in the RNA splicing pathway. This pathway is fundamental for the modification of pre-mRNA into mature and functionally diverse mRNA. Additionally SF3B14 intersects with other spliceosomal proteins such as SF3B1 ensuring correct splice site selection. These interactions facilitate efficient mRNA processing which is essential for the flow of genetic information from DNA to proteins.
- Associated diseases and disorders
Malfunction or dysregulation of SF3B14 has connections with certain cancers including leukemia. Anomalies in the splicing process due to faulty components like SF3B14 can lead to aberrant mRNA and protein products contributing to oncogenic transformation. Additionally SF3B14 has associations with neurodegenerative disorders where disruptions in splicing patterns can affect neural cell survival. The protein SF3B1 closely linked to SF3B14 is often similarly implicated due to their shared roles within the spliceosome.
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SDS-PAGE - Recombinant Human SF3B14 protein (ab103908)
15% SDS-PAGE analysis of ab103908 (3 μg)
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