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AB271736

Recombinant human SF3B2 protein (Active)

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Recombinant human SF3B2 protein (Active) is a Human Fragment protein, in the 401 to 550 aa range, expressed in Escherichia coli, with >75%, suitable for SDS-PAGE, FuncS.

View Alternative Names

SAP145, SF3B2, Splicing factor 3B subunit 2, Pre-mRNA-splicing factor SF3b 145 kDa subunit, Spliceosome-associated protein 145, SF3b145, SAP 145

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Functional Studies - Recombinant human SF3B2 protein (Active) (AB271736)
  • FuncS

Supplier Data

Functional Studies - Recombinant human SF3B2 protein (Active) (AB271736)

Recombinant human SF3B2 protein (Active) (ab271736) activity data.

SDS-PAGE - Recombinant human SF3B2 protein (Active) (AB271736)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human SF3B2 protein (Active) (AB271736)

SDS-PAGE analysis of ab271736.

Key facts

Purity

>75% SDS-PAGE

Expression system

Escherichia coli

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Active

Accession

Q13435

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"45 kDa","actualMolecularWeight":null,"aminoAcidEnd":550,"aminoAcidStart":401,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q13435","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SF3B2 protein also known as SAP145 is a component of the spliceosome complex important for RNA splicing. It has a significant molecular weight of approximately 145 kDa. SF3B2 expresses in various tissues throughout the human body indicating its importance in fundamental cellular activities. The protein mainly localizes to the nucleus where it interacts with other splicing factors to regulate the removal of introns from pre-mRNA.
Biological function summary

This protein plays a critical role in the pre-mRNA splicing process acting as part of the larger spliceosomal complex SF3b which is essential for accurate exon recognition and splice site selection. By ensuring the correct processing of genetic information SF3B2 influences gene expression and stability. It participates in the formation of the spliceosome's U2snRNP sub-complex which is necessary for splicing fidelity and overall cellular function.

Pathways

SF3B2 engages primarily in the mRNA splicing pathway which directly impacts gene expression and regulation. It associates with the splicing factor 3b complex which incorporates multiple proteins for efficient splicing. Its interaction with other spliceosomal proteins such as SF3B1 and U2AF facilitates the understanding of the exact splicing mechanism and highlights SF3B2's central role in these pathways.

Altered SF3B2 expression and function connect to cancer and myelodysplastic syndromes with SF3B1 mutations often observed in similar contexts. Researchers link spliceosomal dysfunctions caused by SF3B2 to aberrant splicing events contributing to oncogenesis and hematological disorders. As a result studies investigate SF3B2 as a potential target for therapeutic intervention focusing on restoring normal splicing activity.
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Specifications

Form

Liquid

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General info

Function

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 12234937, PubMed : 32494006, PubMed : 34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 12234937, PubMed : 32494006, PubMed : 34822310). Within the 17S U2 SnRNP complex, SF3B2 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed : 12234937, PubMed : 27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed : 12234937). May also be involved in the assembly of the 'E' complex (PubMed : 10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077, PubMed : 33509932).

Post-translational modifications

Methylation at Arg-508 by PRMT9 is required for the interaction with SMN1.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 12234937, PubMed : 32494006, PubMed : 34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 12234937, PubMed : 32494006, PubMed : 34822310). Within the 17S U2 SnRNP complex, SF3B2 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed : 12234937, PubMed : 27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed : 12234937). May also be involved in the assembly of the 'E' complex (PubMed : 10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077, PubMed : 33509932).
See full target information SF3B2
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Complementary Products

Primary Antibodies

AB56800

Anti-SF3B2 antibody [5D2]

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