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AB153230

Recombinant Human SF3B3 protein (GST tag N-Terminus)

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Recombinant Human SF3B3 protein (GST tag N-Terminus) is a Human Fragment protein, in the 819 to 929 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

KIAA0017, SAP130, SF3B3, Splicing factor 3B subunit 3, Pre-mRNA-splicing factor SF3b 130 kDa subunit, STAF130, Spliceosome-associated protein 130, SF3b130, SAP 130

1 Images
SDS-PAGE - Recombinant Human SF3B3 protein (GST tag N-Terminus) (AB153230)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SF3B3 protein (GST tag N-Terminus) (AB153230)

ab153230 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q15393

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB209403

Anti-SF3B3 antibody [EPR18441]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B3 antibody [EPR18441] (AB209403)

  • 0
  • 0
Primary Antibodies

AB180144

Anti-CRM1 antibody [EPR14123(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CRM1 antibody [EPR14123(B)] (AB180144)

  • 0
  • 0
Proteins & Peptides

AB238376

Recombinant Rotavirus X VP4 protein (His tag)

SDS-PAGE - Recombinant Rotavirus X VP4 protein (His tag) (AB238376)

  • 0
  • 0
Proteins & Peptides

AB157035

Recombinant Human RBX1 protein

SDS-PAGE - Recombinant Human RBX1 protein (AB157035)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVAKDLILNPRSVAGGFVYTYK","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":929,"aminoAcidStart":819,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q15393","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SF3B3 also known as SAP130 is a component of the splicing factor 3b complex vital for pre-mRNA processing. It is approximately 130 kDa in mass and prominently expressed across various tissue types including the liver lungs and heart. SF3B3 functions in the spliceosome a complex machinery essential for the removal of introns from pre-mRNA transcripts.
Biological function summary

SF3B3 participates in the assembly and stabilization of the functional spliceosomal complex. It is a part of the larger SF3b complex which is critical for recognizing the branch point sequence in RNA during splicing. This recognition influences the selection of splice sites and impacts the regulation of alternative splicing events. The SF3b complex works with the U2 small nuclear ribonucleoprotein (snRNP) during the splicing process to ensure accurate and efficient mRNA maturation.

Pathways

SF3B3 plays a significant role in the splicing pathway and impacts gene expression regulation through alternative splicing. It works alongside proteins such as SF3B1 and other splicing factors maintaining proper cellular function by influencing transcript variants. Another important pathway involving SF3B3 is RNA transport where it assists in the export of mature and correctly processed mRNA from the nucleus to the cytoplasm.

SF3B3 is closely linked to myelodysplastic syndromes and certain types of cancer such as breast cancer. Mutations or dysregulation of SF3B3 can result in aberrant splicing patterns contributing to tumorigenesis and disease progression. The protein is often studied along with SF3B1 as both are part of the splicing machinery and have been implicated in splicing-related genetic abnormalities that drive these disorders.
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Specifications

Form

Liquid

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General info

Function

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 10490618, PubMed : 10882114, PubMed : 12234937, PubMed : 27720643, PubMed : 28781166, PubMed : 32494006, PubMed : 34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 12234937, PubMed : 32494006, PubMed : 34822310). Within the 17S U2 SnRNP complex, SF3B3 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed : 12234937, PubMed : 27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed : 12234937). May also be involved in the assembly of the 'E' complex (PubMed : 10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077, PubMed : 33509932).

Sequence similarities

Belongs to the RSE1 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 10490618, PubMed : 10882114, PubMed : 12234937, PubMed : 27720643, PubMed : 28781166, PubMed : 32494006, PubMed : 34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 12234937, PubMed : 32494006, PubMed : 34822310). Within the 17S U2 SnRNP complex, SF3B3 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed : 12234937, PubMed : 27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed : 12234937). May also be involved in the assembly of the 'E' complex (PubMed : 10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077, PubMed : 33509932).
See full target information SF3B3
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