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AB323018

Recombinant Human SFRP4 Protein Standard (His tag)

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Recombinant Human SFRP4 Protein Standard (His tag) is a Human Full Length protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.
2 Images
Sandwich ELISA - Recombinant Human SFRP4 Protein Standard (His tag) (AB323018)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human SFRP4 Protein Standard (His tag) (AB323018)

Sandwich ELISA with the capture antibody ab285006 dilution at 2 μg/mL and detector antibody ab285112 dilution at 0.5 μg/mL after biotin conjugation.

SDS-PAGE - Recombinant Human SFRP4 Protein Standard (His tag) (AB323018)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SFRP4 Protein Standard (His tag) (AB323018)

SDS-PAGE analysis of ab323018 under reducing conditions for 2ug protein.

Key facts

Purity

>80% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

Q6FHJ7-1

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

Sequence info

[{"linker":null,"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"39.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q6FHJ7","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

Properties and storage information

Form
Liquid
Purification technique
Affinity purification His Tag
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Product protocols

Target data

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types (By similarity). SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (PubMed : 12952927).
See full target information SFRP4

Alternative Names

FRPHE, SFRP4, Secreted frizzled-related protein 4, sFRP-4, FrpHE

Product promise

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