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AB124547

Recombinant Human SGF29 protein (His tag N-Terminus)

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(1 Publication)

Recombinant Human SGF29 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 293 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

CCDC101, SGF29, SAGA-associated factor 29, Coiled-coil domain-containing protein 101, SAGA complex-associated factor 29

1 Images
SDS-PAGE - Recombinant Human SGF29 protein (His tag N-Terminus) (AB124547)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SGF29 protein (His tag N-Terminus) (AB124547)

15% SDS PAGE (3 μg loaded)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q96ES7

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMALVSADSRIAELLTELHQLIKQTQEERSRSEHNLVNIQKTHERMQTENKISPYYRTKLRGLYTTAKADAEAECNILRKALDKIAEIKSLLEERRIAAKIAGLYNDSEPPRKTMRRGVLMTLLQQSAMTLPLWIGKPGDKPPPLCGAIPASGDYVARPGDKVAARVKAVDGDEQWILAEVVSYSHATNKYEVDDIDEEGKERHTLSRRRVIPLPQWKANPETDPEALFQKEQLVLALYPQTTCFYRALIHAPPQRPQDDYSVLFEDTSYADGYSPPLNVAQRYVVACKEPKKK","proteinLength":"Full Length","predictedMolecularWeight":"35.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":293,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96ES7","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SGF29 also known as SAGA-associated factor 29 is a component of the SAGA (Spt-Ada-GCN5 acetyltransferase) coactivator complex which is responsible for transcriptional regulation. The SGF29 protein has a molecular mass of approximately 60 kDa. Researchers have observed its expression in various tissues though it is more prominent in the nucleus where it exerts its functional roles. SGF29 interacts with histone H3 and recognizes the H3K4me3 mark which plays an important role in recruiting other SAGA components for transcription activation.
Biological function summary

The role of SGF29 involves it in chromatin remodeling and transcriptional activation processes. It acts as a scaffold protein binding to methylated histone H3 lysine residues and helps localize the SAGA complex to chromatin sites that are marked for transcription. By attaching to these regions SGF29 facilitates the acetylation of histones a modification that leads to the relaxation of chromatin structure more accessible to transcription machinery.

Pathways

Researchers describe SGF29 as part of the transcriptional regulation pathway and epigenetic modification processes. SGF29 is especially significant in pathways involving the acetylation of histones through the SAGA complex. It works closely with other proteins such as GCN5 and ADA2 which are also components of the SAGA complex. SGF29's interaction with these proteins ensures that acetylation proceeds efficiently influencing gene expression patterns.

SGF29 implications are evident in some cancer types and specific neurodevelopmental disorders. Scientists noticed that aberrant expression or mutations in SGF29 can correlate with tumorigenesis due to misregulated transcription processes. Additionally certain neurodevelopmental disorders can arise when there are disruptions in the pathways SGF29 is involved with potentially affecting cognitive function. These disorders sometimes link SGF29’s abnormal activity with misregulated histone modifications and the modulation of genes associated with neuronal development.
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Specifications

Form

Liquid

Additional notes

Purified by using conventional chromatography techniques.

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General info

Function

Chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes like the TFTC-HAT, ATAC or STAGA complexes (PubMed : 19103755, PubMed : 20850016, PubMed : 21685874, PubMed : 26421618, PubMed : 26578293). SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PubMed : 20850016, PubMed : 21685874, PubMed : 26421618, PubMed : 26578293). In the SAGA-type complexes, SGF29 is required to recruit complexes to H3K4me (PubMed : 20850016). Involved in the response to endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival (PubMed : 23894581). Also binds non-histone proteins that are methylated on Lys residues : specifically recognizes and binds CGAS monomethylated on 'Lys-506' (By similarity).

Sequence similarities

Belongs to the SGF29 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes like the TFTC-HAT, ATAC or STAGA complexes (PubMed : 19103755, PubMed : 20850016, PubMed : 21685874, PubMed : 26421618, PubMed : 26578293). SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PubMed : 20850016, PubMed : 21685874, PubMed : 26421618, PubMed : 26578293). In the SAGA-type complexes, SGF29 is required to recruit complexes to H3K4me (PubMed : 20850016). Involved in the response to endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival (PubMed : 23894581). Also binds non-histone proteins that are methylated on Lys residues : specifically recognizes and binds CGAS monomethylated on 'Lys-506' (By similarity).
See full target information SGF29
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 8:6577 PubMed29700325

2018

KH176 Safeguards Mitochondrial Diseased Cells from Redox Stress-Induced Cell Death by Interacting with the Thioredoxin System/Peroxiredoxin Enzyme Machinery.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Beyrath,Mina Pellegrini,Herma Renkema,Lisanne Houben,Svetlana Pecheritsyna,Peter van Zandvoort,Petra van den Broek,Akkiz Bekel,Pierre Eftekhari,Jan A M Smeitink
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Complementary Products

Primary Antibodies

AB204367

Anti-SGF29 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGF29 antibody - N-terminal (AB204367)

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