Recombinant human SGK2 protein (Active) is a Human Full Length protein, in the 1 to 427 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M N S S P A G T P S P Q P S R A N G N I N L G P S A N P N A Q P T D F D F L K V I G K G N Y G K V L L A K R K S D G A F Y A V K V L Q K K S I L K K K E Q S H I M A E R S V L L K N V R H P F L V G L R Y S F Q T P E K L Y F V L D Y V N G G E L F F H L Q R E R R F L E P R A R F Y A A E V A S A I G Y L H S L N I I Y R D L K P E N I L L D C Q G H V V L T D F G L C K E G V E P E D T T S T F C G T P E Y L A P E V L R K E P Y D R A V D W W C L G A V L Y E M L H G L P P F Y S Q D V S Q M Y E N I L H Q P L Q I P G G R T V A A C D L L Q S L L H K D Q R Q R L G S K A D F L E I K N H V F F S P I N W D D L Y H K R L T P P F N P N V T G P A D L K H F D P E F T Q E A V S K S I G C T P D T V A S S S G A S S A F L G F S Y A P E D D D I L D C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, survival and proliferation. Up-regulates Na(+) channels: SCNN1A/ENAC, K(+) channels: KCNA3/Kv1.3, KCNE1 and KCNQ1, amino acid transporter: SLC6A19, glutamate transporter: SLC1A6/EAAT4, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase.
Alternative names
Serine/threonine-protein kinase Sgk2, Serum/glucocorticoid-regulated kinase 2, SGK2
Recommended products
Recombinant human SGK2 protein (Active) is a Human Full Length protein, in the 1 to 427 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of SGK2 was determined to by 74 nmol/min/mg.
- Accession
- Q9HBY8-2
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF
Sequence info
Amino acid sequence
- M N S S P A G T P S P Q P S R A N G N I N L G P S A N P N A Q P T D F D F L K V I G K G N Y G K V L L A K R K S D G A F Y A V K V L Q K K S I L K K K E Q S H I M A E R S V L L K N V R H P F L V G L R Y S F Q T P E K L Y F V L D Y V N G G E L F F H L Q R E R R F L E P R A R F Y A A E V A S A I G Y L H S L N I I Y R D L K P E N I L L D C Q G H V V L T D F G L C K E G V E P E D T T S T F C G T P E Y L A P E V L R K E P Y D R A V D W W C L G A V L Y E M L H G L P P F Y S Q D V S Q M Y E N I L H Q P L Q I P G G R T V A A C D L L Q S L L H K D Q R Q R L G S K A D F L E I K N H V F F S P I N W D D L Y H K R L T P P F N P N V T G P A D L K H F D P E F T Q E A V S K S I G C T P D T V A S S S G A S S A F L G F S Y A P E D D D I L D C
- Accession
- Q9HBY8
- Protein length
- Full Length
- Predicted molecular weight
- 71 kDa
- Amino acids
- 1 to 427
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, survival and proliferation. Up-regulates Na(+) channels: SCNN1A/ENAC, K(+) channels: KCNA3/Kv1.3, KCNE1 and KCNQ1, amino acid transporter: SLC6A19, glutamate transporter: SLC1A6/EAAT4, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase.
- Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
- Post-translational modifications
Activated by phosphorylation on Ser-356 by an unknown kinase (may be mTORC2 but not confirmed), transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-193.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SGK2 also known as serum/glucocorticoid regulated kinase 2 is a serine/threonine-protein kinase with a molecular mass of approximately 48 kDa. It plays mechanically important roles in cellular ion transport cell growth and survival. SGK2 is part of the larger AGC kinase family and shows a wide tissue distribution with notable expression in the liver pancreas kidney and heart. This kinase is activated in response to cellular stress or growth factors and functions in various cellular contexts.
- Biological function summary
SGK2 contributes to cellular signaling processes that regulate sodium and potassium ion channels impacting cellular homeostasis. SGK2 does not function as part of a larger complex but still exerts significant influence on cell volume regulation and osmoregulation. It modulates activities of ion transporters and other kinases influencing fluid absorption in epithelial cells and metabolic processes highlighting its relevance in both normal physiology and pathophysiology.
- Pathways
SGK2 integrates into signaling networks including the PI3K/AKT pathway and the mTOR pathway. Within these pathways SGK2 serves as a downstream target of PI3K activity participating in cellular responses to insulin and growth factors. The kinase interacts closely with proteins like AKT maintaining cell survival and metabolic balance. SGK2's role in these pathways points to its influence on anabolic processes and adaptive cell responses.
- Associated diseases and disorders
SGK2 has been linked to metabolic syndromes and hypertension. Its involvement in insulin signaling suggests a connection to type 2 diabetes where it interacts with proteins such as AKT2 to affect glucose metabolism and insulin sensitivity. SGK2's regulation of sodium balance also ties it to hypertension with implications for cardiovascular health. Its functional alterations in these contexts emphasize its potential as a therapeutic target in managing metabolic and cardiovascular disorders.
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2 product images
SDS-PAGE - Recombinant human SGK2 protein (Active) (ab261921)
SDS-PAGE analysis of ab261921.
Biological Activity - Recombinant human SGK2 protein (Active) (ab261921)
The specific activity of ab261921 was determines to be 74 nmol/min/mg as per activity assay protocol.
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