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AB271748

Recombinant human SGPL1 protein (Active) (His tag C-Terminus)

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Recombinant human SGPL1 protein (Active) (His tag C-Terminus) is a Human Fragment protein, in the 59 to 568 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KIAA1252, SGPL1, Sphingosine-1-phosphate lyase 1, S1PL, SP-lyase 1, SPL 1, hSPL, Sphingosine-1-phosphate aldolase

2 Images
Functional Studies - Recombinant human SGPL1 protein (Active) (AB271748)
  • FuncS

Supplier Data

Functional Studies - Recombinant human SGPL1 protein (Active) (AB271748)

Specific activity of ab271748.

SDS-PAGE - Recombinant human SGPL1 protein (Active) (AB271748)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human SGPL1 protein (Active) (AB271748)

SDS-PAGE analysis of 2 μg ab271748.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Active

Accession

O95470

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 1.7% Imidazole Constituents: Methionine, PLP, 10% Glycerol (glycerin, glycerine), 2.9% Sodium chloride, 0.79% Tris HCl, 0.03% TCEP HCl

storage-buffer

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Complementary Products

Primary Antibodies

AB224618

Anti-S1P antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P antibody - C-terminal (AB224618)

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  • 3
Proteins & Peptides

AB132072

Recombinant Human S1P1/EDG1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human S1P1/EDG1 protein (AB132072)

  • 0
  • 0
Primary Antibodies

AB302714

Anti-SPHK1 antibody [EPR24511-37]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-SPHK1 antibody [EPR24511-37] (AB302714)

  • 0
  • 0
Primary Antibodies

AB215750

Anti-SPHK2 (phospho T578) antibody

Western blot - Anti-SPHK2 (phospho T578) antibody (AB215750)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"57.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":568,"aminoAcidStart":59,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O95470","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SGPL1 also known as sphingosine-1-phosphate lyase 1 is an enzyme involved in the irreversible breakdown of sphingosine-1-phosphate (S1P). This enzyme has a molecular weight of about 63 kDa. SGPL1 is generally found in the endoplasmic reticulum of cells and is broadly expressed in various tissues including the liver kidney and brain. The key role of SGPL1 is to catalyze the cleavage of S1P an important sphingolipid signaling molecule into phosphoethanolamine and hexadecenal.
Biological function summary

Sphingosine-1-phosphate lyase 1 plays a role in maintaining the balance of sphingolipid metabolites by regulating S1P levels. This protein does not form a known complex but acts alone to facilitate the degradation process. By regulating S1P SGPL1 indirectly influences cell growth survival migration and apoptosis which are processes essential for normal cellular function. The regulation affects numerous cellular systems such as immune response and vascular function.

Pathways

Sphingosine-1-phosphate lyase 1 is integral to the sphingolipid metabolism pathway and the S1P signaling pathway. It directly breaks down S1P therefore diminishing its signaling abilities which are mediated by its interaction with S1P receptors. SGPL1 acts in the final step of sphingolipid degradation and it relates to proteins like sphingosine kinase which phosphorylates sphingosine into S1P therefore connecting two critical steps in the sphingolipid pathway.

Sphingosine-1-phosphate lyase 1 has links to conditions such as nephrotic syndrome and primary immunodeficiency. Mutations in the SGPL1 gene can disrupt sphingolipid balance leading to these diseases. Through these conditions SGPL1 relates to proteins like nephrin which are involved in kidney function and are affected in nephrotic syndrome. Understanding SGPL1's function provides insights into potential therapeutic targets for treating these disorders.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis (PubMed : 11018465, PubMed : 14570870, PubMed : 24809814, PubMed : 28165339). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts. Involved in the regulation of pro-inflammatory response and neutrophil trafficking. Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (By similarity). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).

Sequence similarities

Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.

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Product protocols

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Target data

Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis (PubMed : 11018465, PubMed : 14570870, PubMed : 24809814, PubMed : 28165339). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts. Involved in the regulation of pro-inflammatory response and neutrophil trafficking. Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (By similarity). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).
See full target information SGPL1
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