Recombinant human SGPL1 protein (Active) is a Human Fragment protein, in the 59 to 568 aa range, expressed in Baculovirus infected Sf9, with >=90% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis (PubMed:11018465, PubMed:14570870, PubMed:24809814, PubMed:28165339). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts. Involved in the regulation of pro-inflammatory response and neutrophil trafficking. Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (By similarity). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).
Alternative names
KIAA1252, SGPL1, Sphingosine-1-phosphate lyase 1, S1PL, SP-lyase 1, SPL 1, hSPL, Sphingosine-1-phosphate aldolase
Recommended products
Recombinant human SGPL1 protein (Active) is a Human Fragment protein, in the 59 to 568 aa range, expressed in Baculovirus infected Sf9, with >=90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Active
- Accession
- O95470-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Preservative: 1.7% Imidazole
Constituents: Methionine, PLP, 10% Glycerol (glycerin, glycerine), 2.9% Sodium chloride, 0.79% Tris HCl, 0.03% TCEP HCl
Sequence info
Amino acid sequence
- Accession
- O95470
- Protein length
- Fragment
- Predicted molecular weight
- 57.5 kDa
- Amino acids
- 59 to 568
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis (PubMed:11018465, PubMed:14570870, PubMed:24809814, PubMed:28165339). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts. Involved in the regulation of pro-inflammatory response and neutrophil trafficking. Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (By similarity). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).
- Sequence similarities
Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SGPL1 also known as sphingosine-1-phosphate lyase 1 is an enzyme involved in the irreversible breakdown of sphingosine-1-phosphate (S1P). This enzyme has a molecular weight of about 63 kDa. SGPL1 is generally found in the endoplasmic reticulum of cells and is broadly expressed in various tissues including the liver kidney and brain. The key role of SGPL1 is to catalyze the cleavage of S1P an important sphingolipid signaling molecule into phosphoethanolamine and hexadecenal.
- Biological function summary
Sphingosine-1-phosphate lyase 1 plays a role in maintaining the balance of sphingolipid metabolites by regulating S1P levels. This protein does not form a known complex but acts alone to facilitate the degradation process. By regulating S1P SGPL1 indirectly influences cell growth survival migration and apoptosis which are processes essential for normal cellular function. The regulation affects numerous cellular systems such as immune response and vascular function.
- Pathways
Sphingosine-1-phosphate lyase 1 is integral to the sphingolipid metabolism pathway and the S1P signaling pathway. It directly breaks down S1P therefore diminishing its signaling abilities which are mediated by its interaction with S1P receptors. SGPL1 acts in the final step of sphingolipid degradation and it relates to proteins like sphingosine kinase which phosphorylates sphingosine into S1P therefore connecting two critical steps in the sphingolipid pathway.
- Associated diseases and disorders
Sphingosine-1-phosphate lyase 1 has links to conditions such as nephrotic syndrome and primary immunodeficiency. Mutations in the SGPL1 gene can disrupt sphingolipid balance leading to these diseases. Through these conditions SGPL1 relates to proteins like nephrin which are involved in kidney function and are affected in nephrotic syndrome. Understanding SGPL1's function provides insights into potential therapeutic targets for treating these disorders.
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2 product images
SDS-PAGE - Recombinant human SGPL1 protein (Active) (ab271748)
SDS-PAGE analysis of 2 μg ab271748.
Functional Studies - Recombinant human SGPL1 protein (Active) (ab271748)
Specific activity of ab271748.
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