Recombinant Human SH3GLB2 protein is a Human Full Length protein, in the 1 to 395 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M D F N M K K L A S D A G I F F T R A V Q F T E E K F G Q A E K T E L D A H F E N L L A R A D S T K N W T E K I L R Q T E V L L Q P N P S A R V E E F L Y E K L D R K V P S R V T N G E L L A Q Y M A D A A S E L G P T T P Y G K T L I K V A E A E K Q L G A A E R D F I H T A S I S F L T P L R N F L E G D W K T I S K E R R L L Q N R R L D L D A C K A R L K K A K A A E A K A T T V P D F Q E T R P R N Y I L S A S A S A L W N D E V D K A E Q E L R V A Q T E F D R Q A E V T R L L L E G I S S T H V N H L R C L H E F V K S Q T T Y Y A Q C Y R H M L D L Q K Q L G R F P G T F V G T T E P A S P P L S S T S P T T A A A T M P V V P S V A S L A P P G E A S L C L E E V A P P A S G T R K A R V L Y D Y E A A D S S E L A L L A D E L I T V Y S L P G M D P D W L I G E R G N K K G K V P V T Y L E L L S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
KIAA1848, PP578, SH3GLB2, Endophilin-B2, SH3 domain-containing GRB2-like protein B2
Recommended products
Recombinant Human SH3GLB2 protein is a Human Full Length protein, in the 1 to 395 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9NR46-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris buffered saline, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M D F N M K K L A S D A G I F F T R A V Q F T E E K F G Q A E K T E L D A H F E N L L A R A D S T K N W T E K I L R Q T E V L L Q P N P S A R V E E F L Y E K L D R K V P S R V T N G E L L A Q Y M A D A A S E L G P T T P Y G K T L I K V A E A E K Q L G A A E R D F I H T A S I S F L T P L R N F L E G D W K T I S K E R R L L Q N R R L D L D A C K A R L K K A K A A E A K A T T V P D F Q E T R P R N Y I L S A S A S A L W N D E V D K A E Q E L R V A Q T E F D R Q A E V T R L L L E G I S S T H V N H L R C L H E F V K S Q T T Y Y A Q C Y R H M L D L Q K Q L G R F P G T F V G T T E P A S P P L S S T S P T T A A A T M P V V P S V A S L A P P G E A S L C L E E V A P P A S G T R K A R V L Y D Y E A A D S S E L A L L A D E L I T V Y S L P G M D P D W L I G E R G N K K G K V P V T Y L E L L S
- Accession
- Q9NR46
- Protein length
- Full Length
- Predicted molecular weight
- 46.4 kDa
- Amino acids
- 1 to 395
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab137174 was purified using conventional chromatography techniques.
General info
- Sequence similarities
Belongs to the endophilin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SH3GLB2 also known as Bin1 or Amphiphysin II is a protein involved in membrane dynamics and intracellular trafficking. It has a molecular weight of approximately 40 kDa and is expressed in various tissues with significant expression in the brain heart and skeletal muscle. The protein contains a SH3 domain enabling it to interact with proline-rich motifs of partner proteins. This feature supports its role in processes involving membrane curvature and endocytosis.
- Biological function summary
SH3GLB2 participates in the regulation of membrane remodeling. It acts as part of a larger complex that influences synaptic vesicle endocytosis by facilitating the scission of vesicles from the plasma membrane. This function is critical for maintaining cellular communication and signaling particularly in neuronal cells where rapid membrane trafficking is required. SH3GLB2’s interactions allow for dynamic responses to cellular stress and help in maintaining cellular homeostasis.
- Pathways
This protein is implicated in both the endocytic pathway and apoptotic processes. SH3GLB2 orchestrates interactions within the endocytic pathway alongside proteins such as clathrin and dynamin where it aids in vesicle formation and trafficking. Furthermore it contributes to the apoptotic pathway by modulating factors that influence cell survival and death interacting with proteins like Bax which plays a role in apoptosis regulation. These pathways highlight its involvement in maintaining cellular order and response to external stimuli.
- Associated diseases and disorders
Abnormalities in SH3GLB2 function have been linked to neurodegenerative diseases and certain cancers. In neurodegenerative diseases disrupted SH3GLB2 activity affects synaptic function and neuronal communication potentially contributing to conditions like Alzheimer’s disease. Perturbations in SH3GLB2 interactions with proteins such as tau are noticeable in such disorders. In cancers altered expression or mutation of SH3GLB2 can influence cellular proliferation and invasion mainly through its impact on the endocytic and apoptotic pathways. This connection suggests its role as a potential biomarker or target for therapeutic interventions.
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1 product image
SDS-PAGE - Recombinant Human SH3GLB2 protein (ab137174)
15% SDS-PAGE analysis of ab137174 (3μg)
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