Recombinant human Shh protein (His tag) is a Human Fragment protein, in the 1 to 197 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M L L L A R C L L L V L V S S L L V C S G L A C G P G R G F G K R R H P K K L T P L A Y K Q F I P N V A E K T L G A S G R Y E G K I S R N S E R F K E L T P N Y N P D I I F K D E E N T G A D R L M T Q R C K D K L N A L A I S V M N Q W P G V K L R V T E G W D E D G H H S E E S L H Y E G R A V D I T T S D R D R S K Y G M L A R L A V E A G F D W V Y Y E S K A H I H C S V K A E N S V A A K S G G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Sonic hedgehog protein. The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum (By similarity). Sonic hedgehog protein N-product. The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites (PubMed:24863049). Involved in the patterning of the anterior-posterior axis of the developing limb bud (By similarity). Essential for axon guidance (By similarity). Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (PubMed:10753901). In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO (PubMed:10753901).
Alternative names
Sonic hedgehog protein, SHH, HHG-1, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC
Recommended products
Recombinant human Shh protein (His tag) is a Human Fragment protein, in the 1 to 197 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells.
The ED50 for this effect is typically 2-10 μg/mL.
- Accession
- Q15465-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M L L L A R C L L L V L V S S L L V C S G L A C G P G R G F G K R R H P K K L T P L A Y K Q F I P N V A E K T L G A S G R Y E G K I S R N S E R F K E L T P N Y N P D I I F K D E E N T G A D R L M T Q R C K D K L N A L A I S V M N Q W P G V K L R V T E G W D E D G H H S E E S L H Y E G R A V D I T T S D R D R S K Y G M L A R L A V E A G F D W V Y Y E S K A H I H C S V K A E N S V A A K S G G
- Accession
- Q15465
- Protein length
- Fragment
- Predicted molecular weight
- 21 kDa
- Amino acids
- 1 to 197
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Sonic hedgehog protein
- Sequence similarities
Belongs to the hedgehog family.
- Post-translational modifications
Sonic hedgehog protein
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Functional Studies - Recombinant human Shh protein (His tag) (ab276262)
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells.
The ED50 for this effect is typically 2-10 μg/mL.
SDS-PAGE - Recombinant human Shh protein (His tag) (ab276262)
SDS-PAGE analysis of ab276262
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