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Recombinant human Shh protein (His tag) is a Human Fragment protein, in the 1 to 197 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Alternative names=Sonic hedgehog protein, SHH, HHG-1, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC, SHH
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Sequence
M L L L A R C L L L V L V S S L L V C S G L A C G P G R G F G K R R H P K K L T P L A Y K Q F I P N V A E K T L G A S G R Y E G K I S R N S E R F K E L T P N Y N P D I I F K D E E N T G A D R L M T Q R C K D K L N A L A I S V M N Q W P G V K L R V T E G W D E D G H H S E E S L H Y E G R A V D I T T S D R D R S K Y G M L A R L A V E A G F D W V Y Y E S K A H I H C S V K A E N S V A A K S G G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human Shh protein (His tag) is a Human Fragment protein, in the 1 to 197 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Alternative names=Sonic hedgehog protein, SHH, HHG-1, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC, SHH
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells.
The ED50 for this effect is typically 2-10 μg/mL.
- Predicted molecular weight
21 kDa
- Amino acids
1 to 197
- Sequence
M L L L A R C L L L V L V S S L L V C S G L A C G P G R G F G K R R H P K K L T P L A Y K Q F I P N V A E K T L G A S G R Y E G K I S R N S E R F K E L T P N Y N P D I I F K D E E N T G A D R L M T Q R C K D K L N A L A I S V M N Q W P G V K L R V T E G W D E D G H H S E E S L H Y E G R A V D I T T S D R D R S K Y G M L A R L A V E A G F D W V Y Y E S K A H I H C S V K A E N S V A A K S G G
- Accession
- Q15465-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Specifications
- Form
Lyophilized
General info
- Function
Sonic hedgehog protein
- Sequence similarities
Belongs to the hedgehog family.
- Post-translational modifications
Sonic hedgehog protein
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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