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AB115713

Recombinant Human SHMT1 protein

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Recombinant Human SHMT1 protein is a Human Full Length protein, in the 1 to 483 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT1

1 Images
SDS-PAGE - Recombinant Human SHMT1 protein (AB115713)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human SHMT1 protein (AB115713)

15% SDS-PAGE showing ab115713 (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P34896

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMTMPVNGAHKDADLWSSHDKMLAQPLKDSDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELETLCQKRALQAYKLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYINYDQLEENARLFHPKLIIAGTSCYSRNLEYARLRKIADENGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVKSVDPKTGKEILYNLESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTLEFKVYQHQVVANCRALSEALTELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASFFPLPGLPDF","proteinLength":"Full Length","predictedMolecularWeight":"55.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":483,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P34896","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serine hydroxymethyltransferase 1 (SHMT1) is an enzyme that facilitates the conversion of serine to glycine while simultaneously producing 510-methylenetetrahydrofolate. It's also known by some as cytoplasmic SHMT. The enzyme mass is approximately 53 kDa. SHMT1 is mainly expressed in the cytoplasm of cells though expression can vary across different tissues with significant levels observed in liver and placenta.
Biological function summary

Serine hydroxymethyltransferase 1 plays a significant role in one-carbon metabolism. Particularly it is important for the synthesis of nucleotides which are the building blocks of DNA and RNA. SHMT1 exists in a homotetrameric form implying four SHMT1 units form a single functional enzyme complex. This homotetramer configuration allows it to effectively mediate the important reactions in the cytoplasm contributing to the maintenance of cellular function and proliferation.

Pathways

SHMT1 serves a pivotal role in both the folate and methionine cycles. These pathways are essential for cellular methylation reactions and DNA synthesis. SHMT1 is closely linked to the folate pathway interacting with proteins like methylene tetrahydrofolate reductase (MTHFR) that further utilize 510-methylenetetrahydrofolate produced by SHMT1. Its participation in these pathways highlights its importance in maintaining nucleotide balance and cellular methylation status.

Alterations in SHMT1 levels have been linked to cancer and neural tube defects. In cancer abnormal SHMT1 expression is associated with increased cell proliferation due to its impact on nucleotide synthesis and methylation status. Additionally the interplay between SHMT1 and proteins like thymidylate synthase can influence chemotherapy efficacy. In cases of neural tube defects maternal SHMT1 function can affect embryonic folate metabolism interacting with other proteins such as cystathionine beta-synthase (CBS) impacting folate homeostasis and fetal development.
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Specifications

Form

Liquid

Additional notes

ab115713 was purified using conventional chromatography techniques.

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General info

Function

Interconversion of serine and glycine (PubMed : 24698160, PubMed : 8505317).

Sequence similarities

Belongs to the SHMT family.

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Product protocols

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Target data

Interconversion of serine and glycine (PubMed : 24698160, PubMed : 8505317).
See full target information SHMT1
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Complementary Products

Primary Antibodies

AB186130

Anti-SHMT1 antibody

Western blot - Anti-SHMT1 antibody (AB186130)

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