Recombinant Human SHP2 protein (His tag) is a Human Fragment protein, in the 1 to 460 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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- Signal transduction and targeted therapy 7:3172022Elaiophylin triggers paraptosis and preferentially kills ovarian cancer drug-resistant cells by inducing MAPK hyperactivation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuan-Nan Li,Xue-Jiao Zhao,Zhen Wang,Meng-Shi Luo,Shen-Nan Shi,Dan-Mei Yan,Hua-Yi Li,Jia-Hao Liu,Yang Yang,Jia-Hong Tan,Ze-Yu Zhang,Ru-Qi Chen,Hui-Ling Lai,Xiao-Yuan Huang,Jian-Feng Zhou,Ding Ma,Yong Fang,Qing-Lei GaoPubMed 36097006
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A D P M T S R R W F H P N I T G V E A E N L L L T R G V D G S F L A R P S K S N P G D F T L S V R R N G A V T H I K I Q N T G D Y Y D L Y G G E K F A T L A E L V Q Y Y M E H H G Q L K E K N G D V I E L K Y P L N C A D P T S E R W F H G H L S G K E A E K L L T E K G K H G S F L V R E S Q S H P G D F V L S V R T G D D K G E S N D G K S K V T H V M I R C Q E L K Y D V G G G E R F D S L T D L V E H Y K K N P M V E T L G T V L Q L K Q P L N T T R I N A A E I E S R V R E L S K L A E T T D K V K Q G F W E E F E T L Q Q Q E C K L L Y S R K E G Q R Q E N K N K N R Y K N I L P F D H T R V V L H D G D P N E P V S D Y I N A N I I M P E F E T K C N N S K P K K S Y I A T Q G C L Q N T V N D F W R M V F Q E N S R V I V M T T K E V E R G K S K C V K Y W P D E Y A L K E Y G V M R V R N V K E S A A H D Y T L R E L K L S K V G Q G N T E R T V W Q Y H F R T W P D H G V P S D P G G V L D F L E E V H H K Q E S I M D A G P V V V H C R H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed:10655584, PubMed:14739280, PubMed:18559669, PubMed:18829466, PubMed:26742426, PubMed:28074573). Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed:18559669). Dephosphorylates CDC73 (PubMed:26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity). Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L (PubMed:19275884).
Alternative names
PTP2C, SHPTP2, PTPN11, Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, PTP-1D, PTP-2C, SHP-2, Shp2
Recommended products
Recombinant Human SHP2 protein (His tag) is a Human Fragment protein, in the 1 to 460 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q06124-2
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A D P M T S R R W F H P N I T G V E A E N L L L T R G V D G S F L A R P S K S N P G D F T L S V R R N G A V T H I K I Q N T G D Y Y D L Y G G E K F A T L A E L V Q Y Y M E H H G Q L K E K N G D V I E L K Y P L N C A D P T S E R W F H G H L S G K E A E K L L T E K G K H G S F L V R E S Q S H P G D F V L S V R T G D D K G E S N D G K S K V T H V M I R C Q E L K Y D V G G G E R F D S L T D L V E H Y K K N P M V E T L G T V L Q L K Q P L N T T R I N A A E I E S R V R E L S K L A E T T D K V K Q G F W E E F E T L Q Q Q E C K L L Y S R K E G Q R Q E N K N K N R Y K N I L P F D H T R V V L H D G D P N E P V S D Y I N A N I I M P E F E T K C N N S K P K K S Y I A T Q G C L Q N T V N D F W R M V F Q E N S R V I V M T T K E V E R G K S K C V K Y W P D E Y A L K E Y G V M R V R N V K E S A A H D Y T L R E L K L S K V G Q G N T E R T V W Q Y H F R T W P D H G V P S D P G G V L D F L E E V H H K Q E S I M D A G P V V V H C R H H H H H H
- Accession
- Q06124
- Protein length
- Fragment
- Predicted molecular weight
- 53.9 kDa
- Amino acids
- 1 to 460
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed:10655584, PubMed:14739280, PubMed:18559669, PubMed:18829466, PubMed:26742426, PubMed:28074573). Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed:18559669). Dephosphorylates CDC73 (PubMed:26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity). Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L (PubMed:19275884).
- Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
- Post-translational modifications
Phosphorylated on Tyr-542 and Tyr-580 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SHP2 also known as PTPN11 is a protein tyrosine phosphatase with a molecular mass of approximately 68 kDa. It is expressed in various tissues including the heart liver and immune cells. SHP2 belongs to the non-receptor class of protein tyrosine phosphatases and plays a critical role in cell signaling by acting as a regulator of signal transduction processes. SHP2 mediates these processes by dephosphorylating specific phosphotyrosine residues on target proteins influencing various cellular functions like proliferation differentiation and survival.
- Biological function summary
The role of SHP2 extends to involvement in several signaling cascades such as the Ras/MAPK and PI3K/AKT pathways. It functions as an essential component within protein complexes that facilitate cell communication and response to external signals. The protein modulates growth factor signaling and cytokine signaling highlighting its significance in normal cell function and development. SHP2's statement in signaling processes makes it an important regulator of cellular dynamics.
- Pathways
SHP2 participates in the Ras/MAPK and PI3K/AKT signaling pathways which are important for regulating cell growth survival and differentiation. Within these pathways SHP2 interacts with various signaling molecules including Grb2 Sos and Gab family adaptors. These interactions coordinate cellular responses to growth factors and other extracellular cues ensuring proper pathway activation and control. By serving as a critical mediator SHP2 integrates signals that are necessary for appropriate cellular outcomes.
- Associated diseases and disorders
SHP2 is associated with several conditions such as Noonan syndrome and various cancers. Mutations in the PTPN11 gene which encodes SHP2 often result in aberrant signaling that leads to developmental anomalies or tumorigenesis. In Noonan syndrome the mutated SHP2 protein results in disrupted Ras/MAPK pathway signaling. As for cancers SHP2 is often found to be overactive leading to enhanced cell proliferation and survival. In these contexts SHP2 is interconnected with other proteins like RAS and RAF which also contribute to oncogenic pathway activation and disease progression.
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SDS-PAGE - Recombinant Human SHP2 protein (His tag) (ab227396)
15% SDS-PAGE - 3 μg Recombinant Human SHP2 protein (His tag) (ab227396).
Migrates at 50-70 kDa (SDS-PAGE under reducing conditions).
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