Recombinant Human SI-CLP protein is a Human Full Length protein, in the 1 to 393 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M R T L F N L L W L A L A C S P V H T T L S K S D A K K A A S K T L L E K S Q F S D K P V Q D R G L V V T D L K A E S V V L E H R S Y C S A K A R D R H F A G D V L G Y V T P W N S H G Y D V T K V F G S K F T Q I S P V W L Q L K R R G R E M F E V T G L H D V D Q G W M R A V R K H A K G L H I V P R L L F E D W T Y D D F R N V L D S E D E I E E L S K T V V Q V A K N Q H F D G F V V E V W N Q L L S Q K R V G L I H M L T H L A E A L H Q A R L L A L L V I P P A I T P G T D Q L G M F T H K E F E Q L A P V L D G F S L M T Y D Y S T A H Q P G P N A P L S W V R A C V Q V L D P K S K W R S K I L L G L N F Y G M D Y A T S K D A R E P V V G A R Y I Q T L K D H R P R M V W D S Q V S E H F F E Y K K S R S G R H V V F Y P T L K S L Q V R L E L A R E L G V G V S I W E L G Q G L D Y F Y D L L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro).
Alternative names
GL008, PSEC0104, SB139, CHID1, Chitinase domain-containing protein 1, Stabilin-1-interacting chitinase-like protein, SI-CLP
Recommended products
Recombinant Human SI-CLP protein is a Human Full Length protein, in the 1 to 393 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9BWS9-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M R T L F N L L W L A L A C S P V H T T L S K S D A K K A A S K T L L E K S Q F S D K P V Q D R G L V V T D L K A E S V V L E H R S Y C S A K A R D R H F A G D V L G Y V T P W N S H G Y D V T K V F G S K F T Q I S P V W L Q L K R R G R E M F E V T G L H D V D Q G W M R A V R K H A K G L H I V P R L L F E D W T Y D D F R N V L D S E D E I E E L S K T V V Q V A K N Q H F D G F V V E V W N Q L L S Q K R V G L I H M L T H L A E A L H Q A R L L A L L V I P P A I T P G T D Q L G M F T H K E F E Q L A P V L D G F S L M T Y D Y S T A H Q P G P N A P L S W V R A C V Q V L D P K S K W R S K I L L G L N F Y G M D Y A T S K D A R E P V V G A R Y I Q T L K D H R P R M V W D S Q V S E H F F E Y K K S R S G R H V V F Y P T L K S L Q V R L E L A R E L G V G V S I W E L G Q G L D Y F Y D L L
- Accession
- Q9BWS9
- Protein length
- Full Length
- Amino acids
- 1 to 393
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro).
- Sequence similarities
Belongs to the glycosyl hydrolase 18 family.
- Subcellular localisation
- Lysosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as CHID1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SI-CLP also known as ClpB or Caseinolytic Peptidase B is an ATP-dependent protease with a molecular mass of approximately 95 kDa. It plays a critical role in disaggregating proteins facilitating their refolding in stress conditions. The target is mainly expressed in prokaryotic organisms and some eukaryotic mitochondria. Its mechanism involves using energy derived from ATP hydrolysis to remodel protein aggregates making it essential for maintaining cellular protein homeostasis.
- Biological function summary
SI-CLP proteins interact with other chaperone systems to assist in the recovery of proteins from aggregates. It forms part of a larger Clp/Hsp100 chaperone complex working together with co-chaperones like DnaK and GroEL to efficiently manage protein misfolding. This association with other molecular chaperones allows SI-CLP to execute its role in cellular stress response ensuring damaged or misfolded proteins are effectively processed or refolded.
- Pathways
SI-CLP contributes significantly within the cellular protein quality control and stress response pathways. In these pathways it closely associates with DnaK facilitating protein quality control under stress conditions. Related proteins such as Hsp70 also engage in similar pathways highlighting their coordinated roles in preserving cellular function especially during stress.
- Associated diseases and disorders
SI-CLP proteins have associations with neurodegenerative diseases like Alzheimer’s disease where protein aggregation occurs. It connects with proteins involved in managing protein aggregates such as Hsp70. Furthermore its role in cellular stress responses implicates it in some infectious diseases where pathogens hijack host protein quality control systems. The involvement of SI-CLP in these disorders highlights its potential as a therapeutic target to modulate protein aggregation-related pathologies.
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1 product image
SDS-PAGE - Recombinant Human SI-CLP protein (ab163948)
ab163948 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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Product protocols
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