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AB162734

Recombinant Human SIAE protein

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Recombinant Human SIAE protein is a Human Full Length protein, in the 1 to 523 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

YSG2, SIAE, Sialate O-acetylesterase, H-Lse, Sialic acid-specific 9-O-acetylesterase

1 Images
SDS-PAGE - Recombinant Human SIAE protein (AB162734)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SIAE protein (AB162734)

ab162734 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q9HAT2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MVAPGLVLGLVLPLILWADRSAGIGFRFASYINNDMVLQKEPAGAVIWGFGTPGATVTVTLRQGQETIMKKVTSVKAHSDTWMVVLDPMKPGGPFEVMAQQTLEKINFTLRVHDVLFGDVWLCSGQSNMQMTVLQIFNATRELSNTAAYQSVRILSVSPIQAEQELEDLVAVDLQWSKPTSENLGHGYFKYMSAVCWLFGRHLYDTLQYPIGLIASSWGGTPIEAWSSGRSLKACGVPKQGSIPYDSVTGPSKHSVLWNAMIHPLCNMTLKGVVWYQGESNINYNTDLYNCTFPALIEDWRETFHRGSQGQTERFFPFGLVQLSSDLSKKSSDDGFPQIRWHQTADFGYVPNPKMPNTFMAVAMDLCDRDSPFGSIHPRDKQTVAYRLHLGARALAYGEKNLTFEGPLPEKIELLAHKGLLNLTYYQQIQVQKKDNKIFEISCCSDHRCKWLPASMNTVSTQSLTLAIDSCHGTVVALRYAWTTWPCEYKQCPLYHPSSALPAPPFIAFITDQGPGHQSNVAK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":523,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9HAT2","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sialic acid acetylesterase also known as SIAE is an enzyme that functions by removing acetyl groups from sialic acids on glycoconjugates. This enzyme belongs to the esterase family and exhibits a mass of approximately 60 kDa. SIAE shows expression in various tissues including the liver kidney and brain. It operates by breaking these acetyl linkages which is an important step in regulating sialic acid biology.
Biological function summary

SIAE modifies the sialylation of glycoconjugates which impacts cellular recognition and communication. This enzyme is part of the immune regulation complex influencing how immune cells recognize and interact with each other as well as their environment. SIAE’s activity can alter cell surface properties which affects processes such as cell adhesion migration and signaling.

Pathways

This enzyme appears in sialic acid metabolic pathways and immune response pathways. Within the context of the sialic acid metabolism pathway it works collaboratively with other enzymes like NEU1 and ST6GAL1 impacting the breakdown and synthesis of sialic acid derivatives. Additionally within immune response pathways SIAE influences the function of proteins involved in the regulation of immune cell signaling thereby affecting adaptive and innate immunity.

Researchers have connected SIAE with autoimmune diseases and certain neurodevelopmental disorders. Mutations or dysregulation in SIAE have been linked to conditions like systemic lupus erythematosus (SLE) and autism spectrum disorder (ASD). In the context of SLE SIAE's interaction with proteins like CTLA-4 impacts immune tolerance and autoreactivity. In ASD abnormal sialic acid modifications have implications for neurodevelopmental processes.
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Specifications

Form

Liquid

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General info

Function

Catalyzes the removal of O-acetyl ester groups from position 9 of the free diacetylated sialate N-acetyl-9-O-acetylneuraminate (Neu5,9Ac2) in the cytosol and of the diacetylated sialate residues of sialylglycoconjugates in the lysosomes (Probable). Together with the sialate-O-acetyltransferase they regulate the balance of acetylated sialoglycoconjugates, key players in various processes such as cell-cell interactions, host-pathogen recognition, and tumor antigenicity (PubMed : 21803834).

Subcellular localisation

Lysosome

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Product protocols

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Target data

Catalyzes the removal of O-acetyl ester groups from position 9 of the free diacetylated sialate N-acetyl-9-O-acetylneuraminate (Neu5,9Ac2) in the cytosol and of the diacetylated sialate residues of sialylglycoconjugates in the lysosomes (Probable). Together with the sialate-O-acetyltransferase they regulate the balance of acetylated sialoglycoconjugates, key players in various processes such as cell-cell interactions, host-pathogen recognition, and tumor antigenicity (PubMed : 21803834).
See full target information SIAE
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