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AB131709

Recombinant Human SIAH1 protein

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Recombinant Human SIAH1 protein is a Human Fragment protein, in the 90 to 282 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

HUMSIAH, SIAH1, E3 ubiquitin-protein ligase SIAH1, RING-type E3 ubiquitin transferase SIAH1, Seven in absentia homolog 1, Siah-1a, Siah-1

2 Images
SDS-PAGE - Recombinant Human SIAH1 protein (AB131709)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SIAH1 protein (AB131709)

15% SDS-PAGE showing ab131709 at approximately 24.1 kDa (3µg).

SDS-PAGE - Recombinant Human SIAH1 protein (AB131709)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SIAH1 protein (AB131709)

ab131709 at 3 μg analysed by SDS PAGE.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q8IUQ4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSVANSVLFPCKYASSGCEITLPHTEKADHEELCEFRPYSCPCPGASCKWQGSLDAVMPHLMHQHKSITTLQGEDIVFLATDINLPGAVDWVMMQSCFGFHFMLVLEKQEKYDGHQQFFAIVQLIGTRKQAENFAYRLELNGHRRRLTWEATPRSIHEGIATAIMNSDCLVFDTSIAQLFAENGNLGINVTISMC","proteinLength":"Fragment","predictedMolecularWeight":"24.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":282,"aminoAcidStart":90,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8IUQ4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIAH1 also known as Seven In Absentia Homolog 1 functions mechanically as a RING-type E3 ubiquitin-protein ligase. This protein weighs approximately 32 kDa and adds ubiquitin to target proteins tagging them for proteasomal degradation. Researchers have found SIAH1 expressed in various tissue types including brain heart and kidney. By regulating protein stability SIAH1 plays a significant role in maintaining cellular protein homeostasis.
Biological function summary

SIAH1 influences cell cycle control and apoptosis by interacting with components of protein complexes. It associates with the ubiquitin-proteasome pathway to mediate degradation of key regulatory proteins. SIAH1 helps in reducing cellular proliferation and enhances apoptotic signaling which are vital processes for maintaining balance between cell survival and death. As part of these processes studies have connected SIAH1 to modulating the fate of proteins involved in cell cycle arrest.

Pathways

Several interactions of SIAH1 contribute to signal transduction and apoptosis. One significant pathway involving SIAH1 is the p53 signaling pathway. SIAH1 regulates the degradation of proteins within this pathway including p21 and cyclin D1 which influence cellular responses to DNA damage. Additionally SIAH1 interacts with β-catenin linking it to the Wnt signaling pathway. This interaction affects the regulation of cell growth and differentiation.

SIAH1 has links to cancer and neurodegenerative diseases. In cancer altered SIAH1 expression affects tumor suppressor activity through its interaction with p53 which can lead to uncontrolled cell growth. Moreover SIAH1 connects to neurodegenerative disorders like Parkinson's disease where abnormal protein degradation contributes to neuronal death. In such contexts researchers observe altered interactions between SIAH1 and proteins like parkin a ubiquitin ligase also implicated in these diseases.
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Specifications

Form

Liquid

Additional notes

ab131709 is purified using conventional chromatography techniques

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General info

Function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 28546513, PubMed : 32430360, PubMed : 33591310, PubMed : 9334332, PubMed : 9858595). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP (PubMed : 10747903, PubMed : 11146551, PubMed : 11389839, PubMed : 11389840, PubMed : 11483517, PubMed : 11483518, PubMed : 11752454, PubMed : 12072443). Confers constitutive instability to HIPK2 through proteasomal degradation (PubMed : 18536714, PubMed : 33591310). It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Has some overlapping function with SIAH2 (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Induces apoptosis in cooperation with PEG3 (By similarity). Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus (By similarity). GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins (By similarity). Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4 (By similarity). Also part of the Wnt signaling pathway in which it mediates the Wnt-induced ubiquitin-mediated proteasomal degradation of AXIN1 (PubMed : 28546513, PubMed : 32430360).

Sequence similarities

Belongs to the SINA (Seven in absentia) family.

Post-translational modifications

Phosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and subsequent proteasomal degradation of HIPK2.

Subcellular localisation

Nucleus

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 28546513, PubMed : 32430360, PubMed : 33591310, PubMed : 9334332, PubMed : 9858595). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP (PubMed : 10747903, PubMed : 11146551, PubMed : 11389839, PubMed : 11389840, PubMed : 11483517, PubMed : 11483518, PubMed : 11752454, PubMed : 12072443). Confers constitutive instability to HIPK2 through proteasomal degradation (PubMed : 18536714, PubMed : 33591310). It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Has some overlapping function with SIAH2 (PubMed : 14506261, PubMed : 14645235, PubMed : 14654780, PubMed : 15064394, PubMed : 16085652, PubMed : 19224863, PubMed : 20508617, PubMed : 22483617, PubMed : 9334332, PubMed : 9858595). Induces apoptosis in cooperation with PEG3 (By similarity). Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus (By similarity). GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins (By similarity). Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4 (By similarity). Also part of the Wnt signaling pathway in which it mediates the Wnt-induced ubiquitin-mediated proteasomal degradation of AXIN1 (PubMed : 28546513, PubMed : 32430360).
See full target information SIAH1
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