Recombinant Human Sialidase-3 protein is a Human Fragment protein, in the 411 to 520 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
V T W H H G R L I R P M V T V E C E V A E V T G R A G H P V L Y C S A R T P N R C R A E A L S T D H G E G F Q R L A L S R Q L C E P P H G C Q G S V V S F R P L E I P H R C Q D S S S K D A P T I Q Q S S P G S S L R L E E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3 (PubMed:10405317, PubMed:10861246, PubMed:11298736, PubMed:12011038, PubMed:15847605, PubMed:20511247, PubMed:28646141). Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR (PubMed:17334392, PubMed:25922362). Desialylates EGFR and activates downstream signaling in proliferating cells (PubMed:25922362). Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes (PubMed:26251452).
Alternative names
Sialidase-3, Ganglioside sialidasedis, Membrane sialidase, N-acetyl-alpha-neuraminidase 3, NEU3
Recommended products
Recombinant Human Sialidase-3 protein is a Human Fragment protein, in the 411 to 520 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9UQ49-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- V T W H H G R L I R P M V T V E C E V A E V T G R A G H P V L Y C S A R T P N R C R A E A L S T D H G E G F Q R L A L S R Q L C E P P H G C Q G S V V S F R P L E I P H R C Q D S S S K D A P T I Q Q S S P G S S L R L E E
- Accession
- Q9UQ49
- Protein length
- Fragment
- Amino acids
- 411 to 520
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3 (PubMed:10405317, PubMed:10861246, PubMed:11298736, PubMed:12011038, PubMed:15847605, PubMed:20511247, PubMed:28646141). Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR (PubMed:17334392, PubMed:25922362). Desialylates EGFR and activates downstream signaling in proliferating cells (PubMed:25922362). Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes (PubMed:26251452).
- Sequence similarities
Belongs to the glycosyl hydrolase 33 family.
- Post-translational modifications
Palmitoylated; may regulate intracellular trafficking and anchorage to plasma membrane and endomembranes.
- Subcellular localisation
- Early endosome membrane, Recycling endosome membrane, Lysosome membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human Sialidase-3 protein (ab161170)
ab161170 on a 12.5% SDS-PAGE stained with Coomassie Blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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