Recombinant Human Siglec 7 Protein (Fc-tag) is a Human Fragment protein, in the 19 to 353 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, SDS-PAGE, MS.
Images
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- HPLC, SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
Q K S N R K D Y S L T M Q S S V T V Q E G M C V H V R C S F S Y P V D S Q T D S D P V H G Y W F R A G N D I S W K A P V A T N N P A W A V Q E E T R D R F H L L G D P Q T K N C T L S I R D A R M S D A G R Y F F R M E K G N I K W N Y K Y D Q L S V N V T A L T H R P N I L I P G T L E S G C F Q N L T C S V P W A C E Q G T P P M I S W M G T S V S P L H P S T T R S S V L T L I P Q P Q H H G T S L T C Q V T L P G A G V T T N R T I Q L N V S Y P P Q N L T V T V F Q G E G T A S T A L G N S S S L S V L E G Q S L R L V C A V D S N P P A R L S W T W R S L T L Y P S Q P S N P L V L E L Q V H L G D E G E F T C R A Q N S L G S Q H V S L N L S L Q Q E Y T G K M R P V S G V L L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).
Alternative names
CD328, AIRM1, SIGLEC7, Sialic acid-binding Ig-like lectin 7, Siglec-7, Adhesion inhibitory receptor molecule 1, CDw328, D-siglec, QA79 membrane protein, p75, AIRM-1
Recommended products
Recombinant Human Siglec 7 Protein (Fc-tag) is a Human Fragment protein, in the 19 to 353 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, SDS-PAGE, MS.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- HPLC, SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9Y286-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Q K S N R K D Y S L T M Q S S V T V Q E G M C V H V R C S F S Y P V D S Q T D S D P V H G Y W F R A G N D I S W K A P V A T N N P A W A V Q E E T R D R F H L L G D P Q T K N C T L S I R D A R M S D A G R Y F F R M E K G N I K W N Y K Y D Q L S V N V T A L T H R P N I L I P G T L E S G C F Q N L T C S V P W A C E Q G T P P M I S W M G T S V S P L H P S T T R S S V L T L I P Q P Q H H G T S L T C Q V T L P G A G V T T N R T I Q L N V S Y P P Q N L T V T V F Q G E G T A S T A L G N S S S L S V L E G Q S L R L V C A V D S N P P A R L S W T W R S L T L Y P S Q P S N P L V L E L Q V H L G D E G E F T C R A Q N S L G S Q H V S L N L S L Q Q E Y T G K M R P V S G V L L
- Accession
- Q9Y286
- Protein length
- Fragment
- Predicted molecular weight
- 62.89 kDa
- Actual molecular weight
- 62.76 kDa
- Amino acids
- 19 to 353
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).
- Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
- Post-translational modifications
Tyrosine phosphorylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Product promise
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3 product images
Mass Spectrometry - Recombinant Human Siglec 7 Protein (Fc-tag) (ab307793)
Mass determination by ESI-TOF. Predicted MW is 62891.68 Da (+/- 10 Da by ESI-TOF). Observed MW is 62757.88 Da.
SDS-PAGE - Recombinant Human Siglec 7 Protein (Fc-tag) (ab307793)
SDS-PAGE analysis of ab307793
HPLC - Recombinant Human Siglec 7 Protein (Fc-tag) (ab307793)
HPLC analysis of ab307793
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