Recombinant Human SIGLEC5 protein (Fc Chimera) is a Human Fragment protein, in the 17 to 434 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
E K P V Y E L Q V Q K S V T V Q E G L C V L V P C S F S Y P W R S W Y S S P P L Y V Y W F R D G E I P Y Y A E V V A T N N P D R R V K P E T Q G R F R L L G D V Q K K N C S L S I G D A R M E D T G S Y F F R V E R G R D V K Y S Y Q Q N K L N L E V T A L I E K P D I H F L E P L E S G R P T R L S C S L P G S C E A G P P L T F S W T G N A L S P L D P E T T R S S E L T L T P R P E D H G T N L T C Q M K R Q G A Q V T T E R T V Q L N V S Y A P Q T I T I F R N G I A L E I L Q N T S Y L P V L E G Q A L R L L C D A P S N P P A H L S W F Q G S P A L N A T P I S N T G I L E L R R V R S A E E G G F T C R A Q H P L G F L Q I F L N L S V Y S L P Q L L G P S C S W E A E G L H C R C S F R A R P A P S L C W R L E E K P L E G N S S Q G S F K V N S S S A G P W A N S S L I L H G G L S S D L K V S C K A W N I Y G S Q S G S V L L L Q G R S N L G T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Alternative names
CD170, CD33L2, OBBP2, SIGLEC5, Sialic acid-binding Ig-like lectin 5, Siglec-5, CD33 antigen-like 2, Obesity-binding protein 2, OB-BP2, OB-binding protein 2
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Recombinant Human SIGLEC5 protein (Fc Chimera) is a Human Fragment protein, in the 17 to 434 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O15389-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: L-Arginine, Sodium chloride, 5% Trehalose, 0.75% Glycine, 0.61% Tris
Sequence info
Amino acid sequence
- E K P V Y E L Q V Q K S V T V Q E G L C V L V P C S F S Y P W R S W Y S S P P L Y V Y W F R D G E I P Y Y A E V V A T N N P D R R V K P E T Q G R F R L L G D V Q K K N C S L S I G D A R M E D T G S Y F F R V E R G R D V K Y S Y Q Q N K L N L E V T A L I E K P D I H F L E P L E S G R P T R L S C S L P G S C E A G P P L T F S W T G N A L S P L D P E T T R S S E L T L T P R P E D H G T N L T C Q M K R Q G A Q V T T E R T V Q L N V S Y A P Q T I T I F R N G I A L E I L Q N T S Y L P V L E G Q A L R L L C D A P S N P P A H L S W F Q G S P A L N A T P I S N T G I L E L R R V R S A E E G G F T C R A Q H P L G F L Q I F L N L S V Y S L P Q L L G P S C S W E A E G L H C R C S F R A R P A P S L C W R L E E K P L E G N S S Q G S F K V N S S S A G P W A N S S L I L H G G L S S D L K V S C K A W N I Y G S Q S G S V L L L Q G R S N L G T
- Accession
- O15389
- Protein length
- Fragment
- Predicted molecular weight
- 73 kDa
- Amino acids
- 17 to 434
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
- Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SIGLEC5 also known as CD170 is a sialic acid-binding Ig-like lectin with a molecular mass of approximately 96 kDa. This protein plays an important role in immune cell signaling by recognizing sialylated glycans on the surface of cells. SIGLEC5 is mainly expressed on immune cells such as neutrophils monocytes and macrophages. It belongs to the lectin family of proteins and can modulate immune responses and communication between cells.
- Biological function summary
SIGLEC5 functions by mediating cell-cell interactions and transmitting inhibitory signals that regulate immune responses. It binds to sialic acid-containing glycoproteins which can help modulate the threshold for cell activation by transmitting signals through its immunoreceptor tyrosine-based inhibitory motif (ITIM). SIGLEC5 is not part of a large complex but interacts with other molecules to exert its function in maintaining immune cell balance.
- Pathways
SIGLEC5 plays a role in immune signaling pathways by maintaining cell homeostasis. It contributes to the regulation of the immune response by modulating inhibitory signaling pathways. It is associated with proteins like Src family kinases which phosphorylate the ITIM motif to initiate downstream signaling cascades. Additionally its interaction with sialic acids allows modulation of the innate immune response to various stimuli.
- Associated diseases and disorders
SIGLEC5 has been linked to autoimmune diseases and infections like bacterial sepsis. In autoimmune conditions the dysregulation of SIGLEC5-associated pathways can lead to improper immune cell activation and tissue damage. In the context of bacterial sepsis SIGLEC5 assists in dampening excessive immune responses and limiting inflammation. SIGLEC5 connects with proteins like DAP12 where aberrations could also influence disease progressions making it a target of interest for potential therapeutic interventions.
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1 product image
SDS-PAGE - Recombinant Human SIGLEC5 protein (Fc Chimera) (ab221413)
SDS-PAGE analysis of reduced ab221413 stained overnight with Coomassie Blue.
The protein migrates as 90-105 kDa under reducing conditions (SDS-PAGE) due to glycosylation.
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