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AB89856

Recombinant human SIK2 protein

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Recombinant human SIK2 protein is a Human Full Length protein, expressed in Insect cells, with >70%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

KIAA0781, QIK, SNF1LK2, SIK2, Serine/threonine-protein kinase SIK2, Qin-induced kinase, Salt-inducible kinase 2, Serine/threonine-protein kinase SNF1-like kinase 2, SIK-2

5 Images
Functional Studies - Recombinant human SIK2 protein (AB89856)
  • FuncS

Unknown

Functional Studies - Recombinant human SIK2 protein (AB89856)

Kinase Assay demonstrating specific activity of ab89856

Functional Studies - Recombinant human SIK2 protein (AB89856)
  • FuncS

Unknown

Functional Studies - Recombinant human SIK2 protein (AB89856)

The specific activity of SIK2 (ab89856) was determined to be 37 nmol/min/mg as per activity assay protocol and was equivalent to 128 nmol/min/mg as per radiometric assay

Functional Studies - Recombinant human SIK2 protein (AB89856)
  • FuncS

Unknown

Functional Studies - Recombinant human SIK2 protein (AB89856)

Kinase Assay demonstrating specific activity of ab89856

SDS-PAGE - Recombinant human SIK2 protein (AB89856)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human SIK2 protein (AB89856)

SDS PAGE analysis of ab89856

SDS-PAGE - Recombinant human SIK2 protein (AB89856)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human SIK2 protein (AB89856)

SDS-PAGE showing ab89856 at approximately 150kDa

Key facts

Purity

>70% Densitometry

Expression system

Insect cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS, WB

applications

Biologically active

Yes

Biological activity

The specific activity of ab89856 was determined to be 112 nmol/min/mg.

Accession

Q9H0K1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab204852 (AMARA substrate peptide for SIK + AMPK) can be utilized as a substrate for assessing kinase activity

This product was previously labelled as Snf1lk2, Snf1lk+Snf1lk2+SIK3

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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9H0K1","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIK2 also known as Salt-Inducible Kinase 2 is a protein kinase involved in various cellular processes. It has a molecular mass of approximately 94 kDa. SIK2 is expressed in many tissues with high levels found in adipose tissue liver and skeletal muscle. As a kinase it phosphorylates specific target proteins regulating their activity and influencing cellular mechanisms.
Biological function summary

SIK2 participates in energy metabolism and cellular response to external stress. It interacts with several co-factors to form active complexes facilitating its activity. This protein regulates lipid and carbohydrate metabolism therefore playing a significant role in maintaining cellular energy homeostasis. Additionally SIK2 has a function in controlling the inflammatory response providing a link between metabolism and immune function.

Pathways

SIK2 acts as an essential mediator in the AMPK signaling pathway. This pathway is important for energy balance within cells and is related to other kinases like AMPK and other SIK family members. SIK2 also contributes to the regulation of the cAMP signaling pathway which is linked to various physiological processes. Through these pathways SIK2 influences processes such as glucose uptake and fat oxidation and interacts with proteins like CREB and HDACs.

SIK2 has implications in conditions like obesity and type 2 diabetes. Dysregulation of SIK2 activity can lead to metabolic imbalance and insulin resistance. This kinase is also associated with cancer progression particularly in ovarian cancer where it influences cell proliferation. In these diseases SIK2's connection to proteins such as AMPK and CREB suggests a complex role in disease modulation and potential therapeutic targeting.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Serine/threonine-protein kinase that plays a role in many biological processes such as fatty acid oxidation, autophagy, immune response or glucose metabolism (PubMed : 23322770, PubMed : 26983400). Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators (PubMed : 15454081). Phosphorylates EP300 and thus inhibits its histone acetyltransferase activity (PubMed : 21084751, PubMed : 26983400). In turn, regulates the DNA-binding ability of several transcription factors such as PPARA or MLXIPL (PubMed : 21084751, PubMed : 26983400). Also plays a role in thymic T-cell development (By similarity).

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Post-translational modifications

Phosphorylated at Thr-175 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylated at Thr-484 in response to insulin in adipocytes (PubMed:30586628).. Acetylation at Lys-53 inhibits kinase activity. Deacetylated by HDAC6.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase that plays a role in many biological processes such as fatty acid oxidation, autophagy, immune response or glucose metabolism (PubMed : 23322770, PubMed : 26983400). Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators (PubMed : 15454081). Phosphorylates EP300 and thus inhibits its histone acetyltransferase activity (PubMed : 21084751, PubMed : 26983400). In turn, regulates the DNA-binding ability of several transcription factors such as PPARA or MLXIPL (PubMed : 21084751, PubMed : 26983400). Also plays a role in thymic T-cell development (By similarity).
See full target information SIK2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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