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AB177720

Recombinant Human SIKE1 protein (His tag N-Terminus)

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Recombinant Human SIKE1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 207 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

SIKE, SIKE1, Suppressor of IKBKE 1, Suppressor of IKK-epsilon

1 Images
SDS-PAGE - Recombinant Human SIKE1 protein (His tag N-Terminus) (AB177720)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SIKE1 protein (His tag N-Terminus) (AB177720)

15% SDS PAGE analysis of ab177720 (3 μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9BRV8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMSCTIEKILTDAKTLLERLREHDAAAESLVDQSAALHRRVAAMREAGTALPDQYQEDASDMKDMSKYKPHILLSQENTQIRDLQQENRELWISLEEHQDALELIMSKYRKQMLQLMVAKKAVDAEPVLKAHQSHSAEIESQIDRICEMGEVMRKAVQVDDDQFCKIQEKLAQLELENKELRELLSISSESLQARKENSMDTASQAIK","proteinLength":"Full Length","predictedMolecularWeight":"26.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":207,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9BRV8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIKE1 also known as Squamous Cell Carcinoma Antigen Recognized by T-cells 3 (SART3) is a protein with a molecular weight of approximately 24 kDa. It inhibits the kinase activity of Tank-Binding Kinase 1 (TBK1) and IκB kinase ε (IKKε) which are critical for the induction of type I interferons and other cytokines in response to viral infections. SIKE1 is primarily expressed in various tissues including the brain and liver though expression levels may vary depending on cellular context.
Biological function summary

SIKE1 interacts with signaling pathways to modulate immune responses. As a negative regulator of the interferon signaling pathway it forms complexes with TBK1/IKKε to suppress excessive immune activation preventing potential tissue damage. By efficiently regulating kinase activity SIKE1 helps maintain balance in immune signaling ensuring proper immune defense while avoiding overactivation.

Pathways

The involvement of SIKE1 extends to the toll-like receptor (TLR) and retinoic acid-inducible gene I (RIG-I)-like receptor signaling pathways. These pathways play essential roles in detecting and responding to viral pathogens. SIKE1 modulates the activity of TBK1 and IKKε which are central to the aforementioned pathways impacting downstream molecules like IRF3 and NF-kB that drive interferon production and inflammatory responses.

SiKE1's regulatory role relates to conditions characterized by dysregulated immune responses. For instance in viral infections where excessive interferon production occurs SIKE1 can mitigate potential tissue damage by dampening cytokine output. It is also studied in auto-inflammatory disorders where control of cytokine production is important for managing disease severity. Its connection to TBK1 and IKKε further implicates SIKE1 in the context of these immune-related conditions as these kinases are targets for therapeutic intervention in disorders related to immune dysregulation.
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Specifications

Form

Liquid

Additional notes

ab177720 was purified using conventional chromatography techniques.

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General info

Function

Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and RIGI. Does not inhibit NF-kappa-B activation pathways (PubMed : 16281057). Associates with the striatin-interacting phosphatase and kinase (STRIPAK) core complex, forming the extended (SIKE1 : SLMAP)STRIPAK complex (PubMed : 30622739). The (SIKE1 : SLMAP)STRIPAK complex dephosphorylates STK3 leading to the inhibition of Hippo signaling and the control of cell growth (PubMed : 30622739).

Sequence similarities

Belongs to the SIKE family.

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Product protocols

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Target data

Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and RIGI. Does not inhibit NF-kappa-B activation pathways (PubMed : 16281057). Associates with the striatin-interacting phosphatase and kinase (STRIPAK) core complex, forming the extended (SIKE1 : SLMAP)STRIPAK complex (PubMed : 30622739). The (SIKE1 : SLMAP)STRIPAK complex dephosphorylates STK3 leading to the inhibition of Hippo signaling and the control of cell growth (PubMed : 30622739).
See full target information SIKE1
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