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Recombinant human SIRP alpha protein (Fc Chimera Active) is a Human Fragment protein, in the 31 to 370 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA, HPLC, Flow Cyt.

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Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Fc tag C-Terminus

Applications

SDS-PAGE, FuncS, ELISA, HPLC, Flow Cyt

Biologically active

Yes

Sequence

E E E L Q V I Q P D K S V L V A A G E T A T L R C T A T S L I P V G P I Q W F R G A G P G R E L I Y N Q K E G H F P R V T T V S D L T K R N N M D F S I R I G N I T P A D A G T Y Y C V K F R K G S P D D V E F K S G A G T E L S V R A K P S A P V V S G P A A R A T P Q H T V S F T C E S H G F S P R D I T L K W F K N G N E L S D F Q T N V D P V G E S V S Y S I H S T A K V V L T R E D V H S Q V I C E V A H V T L Q G D P L R G T A N L S E T I R V P P T L E V T Q Q P V R A E N Q V N V T C Q V R K F Y P Q R L Q L T W L E N G N V S R T E T A S T V T E N K D G T Y N W M S W L L V N V S A H R D D V K L T C Q V E H D G Q P A V S K S H D L K V S A H P K E Q G S N T A A E N T G S N E R

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant human SIRP alpha protein (Fc Chimera Active) is a Human Fragment protein, in the 31 to 370 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA, HPLC, Flow Cyt.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS, ELISA, HPLC, Flow Cyt

Biological activity

Measured by its binding ability in a functional ELISA. Recombinant human CD47 protein (Active) (ab174029) at 2 μg/mL (100 μL/well) can bind ab221342 with a linear range of 4-31 ng/mL.

Measured by its binding ability in a functional ELISA. Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into ab221342: Recombinant human CD47 protein (Fc Chimera Active) (Biotin) (ab246018) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2006 μg/mL.

Measured by its binding ability in FACS. The binding of ab221342 to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing anti-CD47 antibody. The concentration of SIRP alpha used is 0.3 μg/ml. IC50=0.1318 μg/ml.

Measured by its binding ability in FACS. Recombinant ab221342 can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 0.3 μg/ml.

Predicted molecular weight

63.5 kDa

Amino acids

31 to 370

Sequence

E E E L Q V I Q P D K S V L V A A G E T A T L R C T A T S L I P V G P I Q W F R G A G P G R E L I Y N Q K E G H F P R V T T V S D L T K R N N M D F S I R I G N I T P A D A G T Y Y C V K F R K G S P D D V E F K S G A G T E L S V R A K P S A P V V S G P A A R A T P Q H T V S F T C E S H G F S P R D I T L K W F K N G N E L S D F Q T N V D P V G E S V S Y S I H S T A K V V L T R E D V H S Q V I C E V A H V T L Q G D P L R G T A N L S E T I R V P P T L E V T Q Q P V R A E N Q V N V T C Q V R K F Y P Q R L Q L T W L E N G N V S R T E T A S T V T E N K D G T Y N W M S W L L V N V S A H R D D V K L T C Q V E H D G Q P A V S K S H D L K V S A H P K E Q G S N T A A E N T G S N E R

Accession
P78324-1
Protein length

Fragment

Animal free

No

Nature

Recombinant

Species

Human

Reconstitution

Reconstitute with sterile deionized water to a concentration of 500 µg/ml.

Concentration
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Storage buffer

pH: 7.4
Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris

Specifications

Form

Lyophilized

Additional notes

>90% pure as determined by SEC-HPLC.Protein A purified.

General info

Function

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Post-translational modifications

N-glycosylated.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

This product is stable after storage at:

  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

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Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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