Recombinant human SIRP alpha protein (Fc Chimera Active) is a Human Fragment protein, in the 31 to 370 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA, HPLC, Flow Cyt.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS, ELISA, HPLC, Flow Cyt
- Biologically active
- Yes
Amino acid sequence
E E E L Q V I Q P D K S V L V A A G E T A T L R C T A T S L I P V G P I Q W F R G A G P G R E L I Y N Q K E G H F P R V T T V S D L T K R N N M D F S I R I G N I T P A D A G T Y Y C V K F R K G S P D D V E F K S G A G T E L S V R A K P S A P V V S G P A A R A T P Q H T V S F T C E S H G F S P R D I T L K W F K N G N E L S D F Q T N V D P V G E S V S Y S I H S T A K V V L T R E D V H S Q V I C E V A H V T L Q G D P L R G T A N L S E T I R V P P T L E V T Q Q P V R A E N Q V N V T C Q V R K F Y P Q R L Q L T W L E N G N V S R T E T A S T V T E N K D G T Y N W M S W L L V N V S A H R D D V K L T C Q V E H D G Q P A V S K S H D L K V S A H P K E Q G S N T A A E N T G S N E R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization (By similarity). Receptor for THBS1 (PubMed:24511121). Interaction with THBS1 stimulates phosphorylation of SIRPA (By similarity). In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production (PubMed:24511121).
Alternative names
CD172a, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP, SIRPA, Tyrosine-protein phosphatase non-receptor type substrate 1, SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, p84, Bit, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3
Recommended products
Recombinant human SIRP alpha protein (Fc Chimera Active) is a Human Fragment protein, in the 31 to 370 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA, HPLC, Flow Cyt.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS, ELISA, HPLC, Flow Cyt
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Recombinant human CD47 protein (Active) (ab174029) at 2 μg/mL (100 μL/well) can bind ab221342 with a linear range of 4-31 ng/mL.
Measured by its binding ability in a functional ELISA. Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into ab221342: Recombinant human CD47 protein (Fc Chimera Active) (Biotin) (ab246018) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2006 μg/mL.
Measured by its binding ability in FACS. The binding of ab221342 to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing anti-CD47 antibody. The concentration of SIRP alpha used is 0.3 μg/ml. IC50=0.1318 μg/ml.
Measured by its binding ability in FACS. Recombinant ab221342 can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 0.3 μg/ml.
- Accession
- P78324-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 500 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris
Sequence info
Amino acid sequence
- E E E L Q V I Q P D K S V L V A A G E T A T L R C T A T S L I P V G P I Q W F R G A G P G R E L I Y N Q K E G H F P R V T T V S D L T K R N N M D F S I R I G N I T P A D A G T Y Y C V K F R K G S P D D V E F K S G A G T E L S V R A K P S A P V V S G P A A R A T P Q H T V S F T C E S H G F S P R D I T L K W F K N G N E L S D F Q T N V D P V G E S V S Y S I H S T A K V V L T R E D V H S Q V I C E V A H V T L Q G D P L R G T A N L S E T I R V P P T L E V T Q Q P V R A E N Q V N V T C Q V R K F Y P Q R L Q L T W L E N G N V S R T E T A S T V T E N K D G T Y N W M S W L L V N V S A H R D D V K L T C Q V E H D G Q P A V S K S H D L K V S A H P K E Q G S N T A A E N T G S N E R
- Accession
- P78324
- Protein length
- Fragment
- Predicted molecular weight
- 63.5 kDa
- Amino acids
- 31 to 370
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
>90% pure as determined by SEC-HPLC.Protein A purified.
General info
- Function
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization (By similarity). Receptor for THBS1 (PubMed:24511121). Interaction with THBS1 stimulates phosphorylation of SIRPA (By similarity). In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production (PubMed:24511121).
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SIRP alpha also known as CD172a is a transmembrane receptor protein with a mass ranging between 70-110 kDa due to glycosylation. This protein extensively expresses on the surface of myeloid cells neurons and a subset of T-cells and is part of the immunoglobulin superfamily. SIRP alpha interacts with its ligand CD47 a widely expressed glycoprotein involved in immune response regulation. Its mechanical action primarily involves signal regulation through the recruitment of SHP-1 and SHP-2 two cytoplasmic tyrosine phosphatases.
- Biological function summary
SIRP alpha functions significantly in the regulation of phagocytosis acting as a "don't eat me" signal to macrophages upon binding with CD47. It does not act alone; rather it is part of a complex that recruits SHP-1 and SHP-2 leading to inhibition of dephosphorylation activities essential for engulfment processes. This regulatory mechanism is important for maintaining cellular homeostasis ensuring that healthy cells are not mistakenly destroyed by the immune system.
- Pathways
SIRP alpha plays an important role in the innate immune pathways involving the regulation of phagocytosis and cell-cell adhesion. Particularly it fits into the immune checkpoint pathways where it interacts closely with proteins like CD47 and plays a role in the interaction between the immune system and cancer cells. Through these pathways SIRP alpha helps maintain balance in the immune response allowing for the recognition of self versus non-self therefore preventing autoimmunity while facilitating the clearance of pathogens.
- Associated diseases and disorders
SIRP alpha is implicated in cancer and autoimmune diseases. In cancer its interaction with CD47 allows tumor cells to escape phagocytosis promoting tumor survival and growth. In autoimmune disorders dysregulated SIRP alpha expression or signaling could miscommunicate immune signals leading to the destruction of healthy tissue. Understanding the link between SIRP alpha and these conditions can reveal potential targets for therapeutic development especially using inhibitors or modulators targeting the SIRP alpha-CD47 interaction.
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6 product images
ELISA - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)
Recombinant human CD47 protein (Active) (Recombinant human CD47 protein (Active) ab174029) at 2 μg/mL (100 μL/well) can bind ab221342 with a linear range of 4-31 ng/mL.
Flow Cytometry - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)
FACS assay shows that recombinant ab221342 can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 0.3 μg/ml.
SDS-PAGE - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)
SDS PAGE of reduced ab221342 stained overnight with Coomassie Blue. The protein migrates as 70-105 kDa under reducing conditions due to glycosylation.
HPLC - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)
The purity of ab221342 was greater than 90% as determined by SEC-HPLC.
ELISA - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)
Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into ab221342: Recombinant human CD47 protein (Fc Chimera Active) (Biotin) (ab246018) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2006 μg/mL.
Functional Studies - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)
FACS analysis shows that the binding of ab221342 to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing anti-CD47 antibody. The concentration of SIRP alpha used is 0.3 μg/ml. IC50=0.1318 μg/ml.
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