JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB235876

Recombinant Human SIRPB1 protein (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human SIRPB1 protein (His tag) is a Human Fragment protein, in the 30 to 371 aa range, expressed in Baculovirus infected insect cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

CD172b, Signal-regulatory protein beta-1, SIRP-beta-1, CD172 antigen-like family member B, SIRPB1

1 Images
SDS-PAGE - Recombinant Human SIRPB1 protein (His tag) (AB235876)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SIRPB1 protein (His tag) (AB235876)

15% SDS-PADE analysis of 3 μg ab235876.

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O00241

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

This product was previously labelled as SIRP beta 1/CD172b
style
left-column

Sequence info

[{"sequence":"EDELQVIQPEKSVSVAAGESATLRCAMTSLIPVGPIMWFRGAGAGRELIYNQKEGHFPRVTTVSELTKRNNLDFSISISNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAVRATPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPAGDSVSYSIHSTARVVLTRGDVHSQVICEIAHITLQGDPLRGTANLSEAIRVPPTLEVTQQPMRAENQANVTCQVSNFYPRGLQLTWLENGNVSRTETASTLIENKDGTYNWMSWLLVNTCAHRDDVVLTCQVEHDGQQAVSKSYALEISAHQKEHGSDITHEAALAPTAPLHHHHHH","proteinLength":"Fragment","predictedMolecularWeight":"38 kDa","actualMolecularWeight":null,"aminoAcidEnd":371,"aminoAcidStart":30,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":"O00241","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Signal regulatory protein beta-1 (SIRPB1) also known as CD172b is a transmembrane glycoprotein that functions as an inhibitory receptor. It weighs about 55 kDa. SIRPB1 is mainly expressed on myeloid cells including monocytes macrophages and granulocytes. It contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) which recruits phosphatases such as Src homology region 2 domain-containing phosphatase-1 (SHP-1) upon phosphorylation. This motif plays an important role in mediating inhibitory signals inside the cell.
Biological function summary

Within the immune system context SIRPB1 acts to regulate functions related to myeloid cells. It does not form part of a larger receptor complex but significantly influences cell communication. It modulates responses like phagocytosis and cellular activation maintaining balanced immune reactions. The interaction with its ligands like CD47 or DAP12 provides insights into its ability to send inhibitory signals contributing to the overall control of immune responses.

Pathways

SIRPB1 gets involved in immune regulation and signal transduction. It plays a significant role in the CD47-SIRP signaling pathway which affects processes like cell migration and phagocytosis. The interaction with SHP-1 and SHP-2 phosphatases signifies its integration within signaling networks governing negative regulation of cellular activation. Through these pathways SIRPB1 interacts with related proteins like SIRPA and SIRPG which have similar functions yet distinct roles within the immune system.

SIRPB1 has associations with inflammatory conditions and cancer. In inflammatory disorders like rheumatoid arthritis altered expression of SIRPB1 can contribute to pathogenesis by modifying immune cell activation further linking it with SIRPA in the modulation of immune responses. In cancer SIRPB1's role extends to tumor immune evasion where upregulated CD47 affects SIRPB1 pathways to inhibit phagocytosis by macrophages influencing tumor progression. Understanding SIRPB1's connections with these proteins and pathways opens avenues for therapeutic interventions in these diseases.
style
left-column

Specifications

Form

Liquid

Additional notes

ab235876 was expressed in insect cell and affinity purified.

style
left-column

General info

Function

Immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Participates also in the recruitment of tyrosine kinase SYK. Triggers activation of myeloid cells when associated with TYROBP (PubMed : 10604985).

Post-translational modifications

N-glycosylated.

style
left-column

Product protocols

style
left-column

Target data

Immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Participates also in the recruitment of tyrosine kinase SYK. Triggers activation of myeloid cells when associated with TYROBP (PubMed : 10604985).
See full target information Signal-regulatory protein beta-1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB234681

Anti-SIRPB1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SIRPB1 antibody (AB234681)

  • 0
  • 0
Proteins & Peptides

AB159886

Recombinant Human ZP2 protein

SDS-PAGE - Recombinant Human ZP2 protein (AB159886)

  • 0
  • 0
Proteins & Peptides

AB162402

Recombinant Human Zinc finger protein 691

SDS-PAGE - Recombinant Human Zinc finger protein 691 (AB162402)

  • 0
  • 0
Proteins & Peptides

AB161146

Recombinant Human BS69 protein

SDS-PAGE - Recombinant Human BS69 protein (AB161146)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com