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AB101134

Recombinant human SIRT5 protein

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(2 Publications)

Recombinant human SIRT5 protein is a Human Full Length protein, in the 1 to 370 aa range, expressed in Baculovirus, with >80%, suitable for SDS-PAGE, FuncS.

View Alternative Names

SIR2L5, SIRT5, Regulatory protein SIR2 homolog 5, SIR2-like protein 5

4 Images
Functional Studies - Recombinant human SIRT5 protein (AB101134)
  • FuncS

Unknown

Functional Studies - Recombinant human SIRT5 protein (AB101134)

The specific activity of SIRT5 (ab101134) was determined to be 23 RLU/min/ng as per activity assay protocol

Functional Studies - Recombinant human SIRT5 protein (AB101134)
  • FuncS

Unknown

Functional Studies - Recombinant human SIRT5 protein (AB101134)

The specific activity of ab101134 was determined to be 20 RLU/min/ng.

SDS-PAGE - Recombinant human SIRT5 protein (AB101134)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human SIRT5 protein (AB101134)

SDS PAGE analysis of ab101134

SDS-PAGE - Recombinant human SIRT5 protein (AB101134)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human SIRT5 protein (AB101134)

SDS-PAGE image showing ab101134 at ~39 kDa.

Key facts

Purity

>80% SDS-PAGE

Expression system

Baculovirus

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab101134 was determined to be 20 RLU/min/ng.

Accession

Q9NXA8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"39 kDa","actualMolecularWeight":null,"aminoAcidEnd":370,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9NXA8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIRT5 also known as Sirtuin 5 is a mitochondrial protein with enzymatic activity as a lysine desuccinylase demalonylase and deglutarylase. It has a molecular mass of approximately 34 kDa. This protein is expressed highly in tissues with high metabolic activity like the liver heart and brain where it participates in regulating energy metabolism. The activity of SIRT5 affects cellular processes by removing post-translational modifications from lysine residues modulating proteins' functions and interactions in these tissues.
Biological function summary

SIRT5 functions as an important regulator of mitochondrial metabolism and influences cellular energy homeostasis. It is not part of larger complexes but it interacts with various metabolic enzymes to control their function. Through its deacylase activity SIRT5 affects the urea cycle and fatty acid oxidation by modifying key enzymes involved in these processes. Its role in maintaining a balance in energy production and consumption highlights its importance in cellular metabolism.

Pathways

SIRT5 is involved in both the tricarboxylic acid (TCA) cycle and the regulation of reactive oxygen species (ROS). Within the TCA cycle SIRT5 desuccinylates and activates enzymes like citrate synthase enhancing the cycle's efficiency. In regulating ROS SIRT5 reduces oxidative stress by affecting enzymes such as superoxide dismutase 2 (SOD2). Its interaction with these pathways and proteins reflects its role in maintaining metabolic health and cellular integrity.

Alterations in SIRT5 levels have been connected to metabolic diseases such as obesity and neurodegenerative disorders like Alzheimer's disease. In obesity SIRT5 dysregulation can lead to inefficient metabolic processes and energy imbalance. In Alzheimer's decreased SIRT5 activity may contribute to increased mitochondrial oxidative stress implicated in disease progression. These connections highlight SIRT5's significance in both metabolic and neurodegenerative contexts linking with proteins like AMPK in obesity and tau proteins in Alzheimer's disease.
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Specifications

Form

Liquid

Additional notes

The purity was determined to be >80% by densitometry. Affinity purified.

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General info

Function

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed : 21908771, PubMed : 22076378, PubMed : 24703693, PubMed : 29180469). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting : acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed : 22076378, PubMed : 24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed : 24140062). Activates SHMT2 by mediating its desuccinylation (PubMed : 29180469). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.

Sequence similarities

Belongs to the sirtuin family. Class III subfamily.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed : 21908771, PubMed : 22076378, PubMed : 24703693, PubMed : 29180469). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting : acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed : 22076378, PubMed : 24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed : 24140062). Activates SHMT2 by mediating its desuccinylation (PubMed : 29180469). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
See full target information SIRT5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 40:66 PubMed39110260

2024

Antitumorigenic potential of Lactobacillus-derived extracellular vesicles: p53 succinylation and glycolytic reprogramming in intestinal epithelial cells via SIRT5 modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jingbo Zhang,Xiumei Huang,Tingting Zhang,Chongqi Gu,Wei Zuo,Lijuan Fu,Yiping Dong,Hao Liu

Cell death and differentiation 29:722-736 PubMed34642466

2021

Repression of p53 function by SIRT5-mediated desuccinylation at Lysine 120 in response to DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Liu,Fangjing Rong,Jinhua Tang,Chunchun Zhu,Xiaoyun Chen,Shuke Jia,Zixuan Wang,Xueyi Sun,Hongyan Deng,Huangyuan Zha,Gang Ouyang,Wuhan Xiao
View all publications
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