Recombinant Human SIX1 protein (denatured) is a Human Full Length protein, in the 1 to 284 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M S M L P S F G F T Q E Q V A C V C E V L Q Q G G N L E R L G R F L W S L P A C D H L H K N E S V L K A K A V V A F H R G N F R E L Y K I L E S H Q F S P H N H P K L Q Q L W L K A H Y V E A E K L R G R P L G A V G K Y R V R R K F P L P R T I W D G E E T S Y C F K E K S R G V L R E W Y A H N P Y P S P R E K R E L A E A T G L T T T Q V S N W F K N R R Q R D R A A E A K E R E N T E N N N S S S N K Q N Q L S P L E G G K P L M S S S E E E F S P P Q S P D Q N S V L L L Q G N M G H A R S S N Y S L P G L T A S Q P S H G L Q T H Q H Q L Q D S L L G P L T S S L V D L G S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development (By similarity). Plays an important role in the development of several organs, including kidney, muscle and inner ear (By similarity). Depending on context, functions as a transcriptional repressor or activator (By similarity). Lacks an activation domain, and requires interaction with EYA family members for transcription activation (PubMed:15141091). Mediates nuclear translocation of EYA1 and EYA2 (PubMed:19497856). Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter and CIDEA enhancer (PubMed:15141091, PubMed:19497856, PubMed:23435380, PubMed:27923061). Regulates the expression of numerous genes, including MYC, CCND1 and EZR (By similarity). Acts as an activator of the IGFBP5 promoter, probably coactivated by EYA2 (By similarity). Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex (By similarity). During myogenesis, seems to act together with EYA2 and DACH2 (By similarity). Regulates the expression of CCNA1 (PubMed:15123840). Promotes brown adipocyte differentiation (By similarity).
Alternative names
Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX1
Recommended products
Recombinant Human SIX1 protein (denatured) is a Human Full Length protein, in the 1 to 284 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q15475-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 12.01% Urea, 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M S M L P S F G F T Q E Q V A C V C E V L Q Q G G N L E R L G R F L W S L P A C D H L H K N E S V L K A K A V V A F H R G N F R E L Y K I L E S H Q F S P H N H P K L Q Q L W L K A H Y V E A E K L R G R P L G A V G K Y R V R R K F P L P R T I W D G E E T S Y C F K E K S R G V L R E W Y A H N P Y P S P R E K R E L A E A T G L T T T Q V S N W F K N R R Q R D R A A E A K E R E N T E N N N S S S N K Q N Q L S P L E G G K P L M S S S E E E F S P P Q S P D Q N S V L L L Q G N M G H A R S S N Y S L P G L T A S Q P S H G L Q T H Q H Q L Q D S L L G P L T S S L V D L G S
- Accession
- Q15475
- Protein length
- Full Length
- Predicted molecular weight
- 34.7 kDa
- Amino acids
- 1 to 284
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development (By similarity). Plays an important role in the development of several organs, including kidney, muscle and inner ear (By similarity). Depending on context, functions as a transcriptional repressor or activator (By similarity). Lacks an activation domain, and requires interaction with EYA family members for transcription activation (PubMed:15141091). Mediates nuclear translocation of EYA1 and EYA2 (PubMed:19497856). Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter and CIDEA enhancer (PubMed:15141091, PubMed:19497856, PubMed:23435380, PubMed:27923061). Regulates the expression of numerous genes, including MYC, CCND1 and EZR (By similarity). Acts as an activator of the IGFBP5 promoter, probably coactivated by EYA2 (By similarity). Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex (By similarity). During myogenesis, seems to act together with EYA2 and DACH2 (By similarity). Regulates the expression of CCNA1 (PubMed:15123840). Promotes brown adipocyte differentiation (By similarity).
- Sequence similarities
Belongs to the SIX/Sine oculis homeobox family.
- Post-translational modifications
Phosphorylated during interphase; becomes hyperphosphorylated during mitosis. Hyperphosphorylation impairs binding to promoter elements.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SIX1 also known as Sine oculis homeobox homolog 1 is a transcription factor with a molecular mass of about 32 kDa. SIX1 plays an important role in regulating gene expression by binding to DNA sequences. This transcription factor shows expression in various tissues including skeletal muscle kidney and neural tissues. In developing tissues the expression is particularly significant indicating its role during embryonic development and differentiation.
- Biological function summary
SIX1 influences cell proliferation survival and differentiation. As part of a transcriptional complex it interacts frequently with cofactors like EYA1 to execute its roles in developmental processes. These interactions allow SIX1 to impact gene targets that are important for tissue morphogenesis. The malfunctioning of these processes can lead to developmental disorders highlighting its role in the regulatory networks that govern organogenesis.
- Pathways
Several essential biological processes involve SIX1 including the Wnt/β-catenin and TGF-β signaling pathways. In these pathways SIX1 sometimes regulates or interacts with proteins such as β-catenin enhancing transcriptional activation essential for cell fate determination. In the TGF-β pathway SIX1 influences epithelial-mesenchymal transition (EMT) an important event in embryogenesis and metastasis. These pathway involvements indicate its importance in maintaining cellular homeostasis and signaling balance.
- Associated diseases and disorders
SIX1 has a significant link to cancer and congenital anomalies. Overexpression or mutations of SIX1 are often implicated in different cancers such as breast cancer due to its role in facilitating EMT and increasing invasiveness. Moreover SIX1 mutations associate with Branchio-Oto-Renal (BOR) syndrome a developmental disorder that affects the kidneys ears and branchial arches. In these contexts SIX1 connects with other proteins like β-catenin in cancer pathways and EYA1 in BOR syndrome further illustrating its broad impact on health and disease.
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1 product image
SDS-PAGE - Recombinant Human SIX1 protein (denatured) (ab134521)
15% SDS-PAGE analysis of ab134521 (3ug)
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