JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB177610

Recombinant Human SKA1 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human SKA1 protein is a Human Full Length protein, in the 1 to 255 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

C18orf24, SKA1, SKA complex subunit 1, Spindle and kinetochore-associated protein 1

1 Images
SDS-PAGE - Recombinant Human SKA1 protein (AB177610)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SKA1 protein (AB177610)

ab177610 (3ug) on a 15% SDS-PAGE.

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q96BD8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMASSDLEQLCSHVNEKIGNIKKTLSLRNCGQEPTLKTVLNKIGDEIIVINELLNKLELEIQYQEQTNNSLKELCESLEEDYKDIEHLKENVPSHLPQVTVTQSCVKGSDLDPEEPIKVEEPEPVKKPPKEQRSIKEMPFITCDEFNGVPSYMKSRLTYNQINDVIKEINKAVISKYKILHQPKKSMNSVTRNLYHRFIDEETKDTKGRYFIVEADIKEFTTLKADKKFHVLLNILRHCRRLSEVRGGGLTRYVIT","proteinLength":"Full Length","predictedMolecularWeight":"31.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":255,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96BD8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spindle and kinetochore associated protein 1 (SKA1) is part of a complex known for its essential role in the mitotic spindle checkpoint. SKA1 sometimes referred to by its alternate name Protein CASC5 has a molecular weight of approximately 9.5 kDa. SKA1 mainly expresses in tissues where cell division is active which include bone marrow and various cancerous tissues. Its function connects it to the assemblies of microtubules and kinetochores ensuring accurate chromosome segregation during mitosis.
Biological function summary

SKA1 interacts with other proteins to maintain the stability of the spindle microtubules attached to kinetochores. It forms the SKA complex with SKA2 and SKA3 which helps anchor microtubule fibers to kinetochores. This interaction is critical for tracking kinetochores along the microtubules during mitosis promoting proper chromosome alignment and segregation. The SKA complex ensures that daughter cells receive the correct number of chromosomes after cell division facilitating genomic integrity.

Pathways

SKA1 participates in the mechanisms governing mitosis and is involved in the progression through the cell cycle. It operates closely with the spindle assembly checkpoint pathway relying on interactions with NDC80 and SPC24 within the kinetochore complex. These interactions are necessary for monitoring chromosome alignment and preventing premature entry into anaphase. SKA1 supports the processes that control cell division fidelity making it vital to understanding normal cell cycle and division.

SKA1 associates strongly with various cancers such as breast and colorectal cancers. Abnormal expression or malfunction of SKA1 can lead to incorrect chromosome segregation contributing to aneuploidy a condition frequently seen in cancer cells. SKA1 interactions with proteins like MAD2 a mitotic checkpoint protein are implicated in tumorigenesis due to their roles in cell cycle control. Investigating SKA1 offers insights into potential therapeutic interventions especially targeting cell division in cancer treatment.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Component of the SKA complex, a microtubule plus end-binding complex of the outer kinetochore that stabilizes spindle microtubule-kinetochore attachments, promotes alignment of chromosomes at the mitotic spindle equator (chromosome congression) and assists suppression of the spindle assembly checkpoint (PubMed : 17093495, PubMed : 19289083, PubMed : 22371557, PubMed : 22483620, PubMed : 23085020, PubMed : 26981768, PubMed : 27697923, PubMed : 29487209, PubMed : 31804178). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed : 19289083, PubMed : 22483620, PubMed : 23085020, PubMed : 28479321, PubMed : 29487209). The outer kinetochore is made up of the ten-subunit KMN network complex, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components such as the SKA complex; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (PubMed : 17093495, PubMed : 19289083, PubMed : 23085020, PubMed : 28479321, PubMed : 29487209). The SKA complex is loaded onto bioriented kinetochores and it facilitates chromosome congression by stabilizing microtubules together with MAPRE1, and end-on attachment of the NDC80 complex to depolymerizing spindle microtubules, thereby assisting the poleward-moving kinetochore in withstanding microtubule pulling forces (PubMed : 19289083, PubMed : 22371557, PubMed : 22454517, PubMed : 23085020, PubMed : 24413531, PubMed : 27697923, PubMed : 28479321, PubMed : 28495837, PubMed : 29487209). The complex associates with dynamic microtubule plus-ends and can track both depolymerizing and elongating microtubules (PubMed : 23085020, PubMed : 29153323). The complex recruits protein phosphatase 1 (PP1) to the kinetochore in prometaphase and metaphase, to oppose spindle assembly checkpoint signaling and promote the onset of anaphase (PubMed : 26981768). In the complex, it mediates interactions with microtubules (PubMed : 19289083, PubMed : 22483620, PubMed : 23085020, PubMed : 24413531, PubMed : 27667719, PubMed : 29153323, PubMed : 36592928). It also stimulates AURKB/Aurora B catalytic activity (PubMed : 27697923). During meiosis the SKA complex stabilizes the meiotic spindle and is required for its migration to the cortex (By similarity).

Sequence similarities

Belongs to the SKA1 family.

Post-translational modifications

Phosphorylated by AURKB at Thr-157 and Ser-242 which negatively regulates the association of the SKA complex with kinetochores to allow correction of aberrant kinetochore-microtubule interactions and promote mitotic sister chromatid biorientation.

Subcellular localisation

Cytoskeleton

style
left-column

Product protocols

style
left-column

Target data

Component of the SKA complex, a microtubule plus end-binding complex of the outer kinetochore that stabilizes spindle microtubule-kinetochore attachments, promotes alignment of chromosomes at the mitotic spindle equator (chromosome congression) and assists suppression of the spindle assembly checkpoint (PubMed : 17093495, PubMed : 19289083, PubMed : 22371557, PubMed : 22483620, PubMed : 23085020, PubMed : 26981768, PubMed : 27697923, PubMed : 29487209, PubMed : 31804178). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed : 19289083, PubMed : 22483620, PubMed : 23085020, PubMed : 28479321, PubMed : 29487209). The outer kinetochore is made up of the ten-subunit KMN network complex, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components such as the SKA complex; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (PubMed : 17093495, PubMed : 19289083, PubMed : 23085020, PubMed : 28479321, PubMed : 29487209). The SKA complex is loaded onto bioriented kinetochores and it facilitates chromosome congression by stabilizing microtubules together with MAPRE1, and end-on attachment of the NDC80 complex to depolymerizing spindle microtubules, thereby assisting the poleward-moving kinetochore in withstanding microtubule pulling forces (PubMed : 19289083, PubMed : 22371557, PubMed : 22454517, PubMed : 23085020, PubMed : 24413531, PubMed : 27697923, PubMed : 28479321, PubMed : 28495837, PubMed : 29487209). The complex associates with dynamic microtubule plus-ends and can track both depolymerizing and elongating microtubules (PubMed : 23085020, PubMed : 29153323). The complex recruits protein phosphatase 1 (PP1) to the kinetochore in prometaphase and metaphase, to oppose spindle assembly checkpoint signaling and promote the onset of anaphase (PubMed : 26981768). In the complex, it mediates interactions with microtubules (PubMed : 19289083, PubMed : 22483620, PubMed : 23085020, PubMed : 24413531, PubMed : 27667719, PubMed : 29153323, PubMed : 36592928). It also stimulates AURKB/Aurora B catalytic activity (PubMed : 27697923). During meiosis the SKA complex stabilizes the meiotic spindle and is required for its migration to the cortex (By similarity).
See full target information SKA1
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB174559

Recombinant Human SSSCA1 protein (denatured)

SDS-PAGE - Recombinant Human SSSCA1 protein (denatured) (AB174559)

  • 0
  • 0
Primary Antibodies

AB91550

Anti-SKA1 antibody

Western blot - Anti-SKA1 antibody (AB91550)

  • 0
  • 0
Proteins & Peptides

AB177632

Recombinant Human Ubiquinol-Cytochrome C Reductase Core Protein I (denatured)

SDS-PAGE - Recombinant Human Ubiquinol-Cytochrome C Reductase Core Protein I (denatured) (AB177632)

  • 0
  • 0
Proteins & Peptides

AB177641

Recombinant Human XRCC2 protein (denatured)

SDS-PAGE - Recombinant Human XRCC2 protein (denatured) (AB177641)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com