Recombinant Human Skp1 protein (Tag Free)
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Recombinant Human Skp1 protein (Tag Free) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.
View Alternative Names
EMC19, OCP2, SKP1A, TCEB1L, SKP1, S-phase kinase-associated protein 1, Cyclin-A/CDK2-associated protein p19, Organ of Corti protein 2, Organ of Corti protein II, RNA polymerase II elongation factor-like protein, SIII, Transcription elongation factor B polypeptide 1-like, p19skp1, p19A, OCP-2, OCP-II
- WB
Unknown
Western blot - Recombinant Human Skp1 protein (Tag Free) (AB90687)
All lanes:
Anti-Skp1 antibody (<a href='/en-us/products/unavailable/skp1-antibody-ab10546'>ab10546</a>) at 1/500 dilution
All lanes:
Western blot - Recombinant Human Skp1 protein (Tag Free) (ab90687) at 0.01 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution
true
Exposure time: 20s
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human Skp1 protein (AB90687)
15% SDS-PAGE showing ab90687 (3μg).
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Liquid
Additional notes
ab90687 is purified using conventional chromatography techniques.
General info
Function
Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2 (PubMed : 25704143). SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2. Core component of the Cul7-RING(FBXW8) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 35982156).
Sequence similarities
Belongs to the SKP1 family.
Post-translational modifications
Undergoes autophagy-mediated degradation in the liver in a time-dependent manner.
Target data
Complementary Products
![Flow Cytometry (Intracellular) - Anti-Skp1 antibody [EPR3304] (AB76502)](https://content.abcam.com/products/images/skp1-antibody-epr3304-ab76502--flow-cytometry-intracellular-img38004.jpg)
Product promise
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