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AB166162

Recombinant Human SLC13A5 protein (GST tag N-Terminus)

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Recombinant Human SLC13A5 protein (GST tag N-Terminus) is a Human Fragment protein, in the 152 to 206 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

NACT, SLC13A5, Na(+)/citrate cotransporter, NaCT, Sodium-coupled citrate transporter, Sodium-dependent citrate transporter, Solute carrier family 13 member 5

1 Images
SDS-PAGE - Recombinant Human SLC13A5 protein (GST tag N-Terminus) (AB166162)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SLC13A5 protein (GST tag N-Terminus) (AB166162)

ab166162 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q86YT5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"VEAILQQMEATSAATEAGLELVDKGKAKELPGSQVIFEGPTLGQQEDQERKRLCK","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":206,"aminoAcidStart":152,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q86YT5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC13A5 also known as the sodium/citrate cotransporter is an important transporter that facilitates the uptake of citrate across cellular membranes. This protein is also referred to as NaCT and has a molecular mass of approximately 62 kDa. Researchers found its expression most notably in the liver brain and testes. SLC13A5 operates by coupling the inward movement of sodium ions with the transport of citrate which is an intermediary in metabolic pathways.
Biological function summary

SLC13A5 plays an important role in cellular metabolism by importing citrate into cells which then serves as a precursor for fatty acid cholesterol and glucose synthesis. The protein does not form part of a multi-protein complex but functions individually to regulate citrate levels within the cell. By modulating intracellular citrate SLC13A5 influences the balance between energy production and storage.

Pathways

SLC13A5 contributes directly to the citrate shuttle and indirectly influences the tricarboxylic acid (TCA) cycle. In the citrate shuttle pathway citrate transported by SLC13A5 provides carbon for the biosynthesis of lipids. This transport influences pathways involving acetyl-CoA production. Additionally it interacts with proteins such as ATP citrate lyase which converts citrate to acetyl-CoA within the cytosol impacting lipid metabolism pathways.

Researchers have linked SLC13A5 to early infantile epileptic encephalopathy (EIEE) a neurological disorder characterized by severe seizures. Mutations in the SLC13A5 gene reduce or eliminate the transport of citrate leading to metabolic disruptions. Additionally studies suggest links between dysregulated citrate transport and metabolic disorders like non-alcoholic fatty liver disease (NAFLD). The protein may interact with acyl-CoA synthetase short-chain family member 2 (ACSS2) in these contexts suggesting a broader impact on fatty acid metabolism.
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Specifications

Form

Liquid

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General info

Function

High-affinity sodium/citrate cotransporter that mediates the entry of citrate into cells, which is a critical participant of biochemical pathways (PubMed : 12445824, PubMed : 12826022, PubMed : 26324167, PubMed : 26384929, PubMed : 30054523, PubMed : 33597751, PubMed : 39622972). May function in various metabolic processes in which citrate has a critical role such as energy production (Krebs cycle), fatty acid synthesis, cholesterol synthesis, glycolysis, and gluconeogenesis (PubMed : 12826022). Transports citrate into the cell in a Na(+)-dependent manner, recognizing the trivalent form of citrate (physiological pH) rather than the divalent form (PubMed : 12445824, PubMed : 12826022, PubMed : 26324167, PubMed : 26384929, PubMed : 30054523, PubMed : 33597751, PubMed : 39622972). Can recognize succinate as a substrate, but its affinity for succinate is several fold lower than for citrate (PubMed : 26324167). The stoichiometry is probably 4 Na(+) for each carboxylate, irrespective of whether the translocated substrate is divalent or trivalent, rendering the process electrogenic (PubMed : 12445824, PubMed : 12826022, PubMed : 39622972). Involved in the regulation of citrate levels in the brain (By similarity).

Sequence similarities

Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.

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Product protocols

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Target data

High-affinity sodium/citrate cotransporter that mediates the entry of citrate into cells, which is a critical participant of biochemical pathways (PubMed : 12445824, PubMed : 12826022, PubMed : 26324167, PubMed : 26384929, PubMed : 30054523, PubMed : 33597751, PubMed : 39622972). May function in various metabolic processes in which citrate has a critical role such as energy production (Krebs cycle), fatty acid synthesis, cholesterol synthesis, glycolysis, and gluconeogenesis (PubMed : 12826022). Transports citrate into the cell in a Na(+)-dependent manner, recognizing the trivalent form of citrate (physiological pH) rather than the divalent form (PubMed : 12445824, PubMed : 12826022, PubMed : 26324167, PubMed : 26384929, PubMed : 30054523, PubMed : 33597751, PubMed : 39622972). Can recognize succinate as a substrate, but its affinity for succinate is several fold lower than for citrate (PubMed : 26324167). The stoichiometry is probably 4 Na(+) for each carboxylate, irrespective of whether the translocated substrate is divalent or trivalent, rendering the process electrogenic (PubMed : 12445824, PubMed : 12826022, PubMed : 39622972). Involved in the regulation of citrate levels in the brain (By similarity).
See full target information SLC13A5
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