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AB314628

Recombinant Human SLC14A1 Protein (His Tag)

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Recombinant Human SLC14A1 Protein (His Tag) is a Human Full Length protein, in the 1 to 389 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

HUT11, JK, RACH1, UT1, UTE, SLC14A1, Urea transporter 1, Solute carrier family 14 member 1

1 Images
SDS-PAGE - Recombinant Human SLC14A1 Protein (His Tag) (AB314628)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human SLC14A1 Protein (His Tag) (AB314628)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

10x His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q13336

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Storage buffer

pH: 7.4 - 8 Constituents: PBS, 6% Trehalose, 0.05% dodecyl 2-(trimethylazaniumyl)ethyl phosphate

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Complementary Products

Primary Antibodies

AB103295

Anti-SLC14A1/UTE antibody

Western blot - Anti-SLC14A1/UTE antibody (AB103295)

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  • 0
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB254072

Anti-Aquaporin 2 (phospho S269) antibody

Western blot - Anti-Aquaporin 2 (phospho S269) antibody (AB254072)

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  • 0
Proteins & Peptides

AB109943

Recombinant Human Rab3D protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Rab3D protein (His tag N-Terminus) (AB109943)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MEDSPTMVRVDSPTMVRGENQVSPCQGRRCFPKALGYVTGDMKELANQLKDKPVVLQFIDWILRGISQVVFVNNPVSGILILVGLLVQNPWWALTGWLGTVVSTLMALLLSQDRSLIASGLYGYNATLVGVLMAVFSDKGDYFWWLLLPVCAMSMTCPIFSSALNSMLSKWDLPVFTLPFNMALSMYLSATGHYNPFFPAKLVIPITTAPNISWSDLSALELLKSIPVGVGQIYGCDNPWTGGIFLGAILLSSPLMCLHAAIGSLLGIAAGLSLSAPFENIYFGLWGFNSSLACIAMGGMFMALTWQTHLLALGCALFTAYLGVGMANFMAEVGLPACTWPFCLATLLFLIMTTKNSNIYKMPLSKVTYPEENRIFYLQAKKRMVESPL","proteinLength":"Full Length","predictedMolecularWeight":"44 kDa","actualMolecularWeight":null,"aminoAcidEnd":389,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q13336","tags":[{"tag":"10x His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
Storage information
The product can be stored for up to 12 months
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC14A1 also known as UTE is a protein that functions as a urea transporter. It facilitates the movement of urea across cell membranes specifically in the kidney and erythrocytes. It has a molecular weight of approximately 50 kDa. The expression of SLC14A1 is notable in the renal medulla and red blood cells where it plays a role in maintaining the body's urea balance. This transporter contributes to urine concentration and detoxification processes by efficiently regulating urea levels within different tissues and body fluids.
Biological function summary

The function of SLC14A1 in transporting urea is important for nitrogen waste removal. It operates within renal epithelial cells to assist with the reabsorption of urea from urine back into the bloodstream contributing to water conservation and nitrogen excretion. SLC14A1 is not known to be part of a larger protein complex but interacts with other renal transporters to enhance its role in concentrating urine. This transport activity helps in creating the osmotic gradient necessary for the kidney's urine concentrating mechanism.

Pathways

SLC14A1 integrates into the renal system's water and urea reabsorption processes. It is pivotal in the urea cycle and the renal concentrating mechanism pathways. Its functionality is closely linked with aquaporin channels which are responsible for water reabsorption in the kidney. These pathways coordinate to regulate body fluid and electrolyte balance establishing a direct relationship between SLC14A1 and aquaporins to optimize urea transport and water reabsorption throughout the physiological system.

SLC14A1's impaired function is linked to urea transport dysfunctions such as urinary concentrating deficiency which can lead to conditions like nephrogenic diabetes insipidus. Defects or mutations in its gene can disrupt its transporter activity and affect the body's ability to concentrate urine leading to polyuria and dehydration. In certain scenarios altered activity of SLC14A1 might also interact with conditions related to anemia where urea transport in erythrocytes plays a role in maintaining red blood cell function and stability.
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Specifications

Form

Lyophilized

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General info

Function

Mediates the transport of urea driven by a concentration gradient across the cell membrane of erythrocytes (PubMed : 10514515, PubMed : 7797558, PubMed : 7989337, PubMed : 8997401). Also mediates the transport of urea across the cell membrane of the renal inner medullary collecting duct which is critical to the urinary concentrating mechanism (By similarity). Facilitates water transport in erythrocytes (By similarity).

Sequence similarities

Belongs to the urea transporter family.

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Product protocols

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Target data

Mediates the transport of urea driven by a concentration gradient across the cell membrane of erythrocytes (PubMed : 10514515, PubMed : 7797558, PubMed : 7989337, PubMed : 8997401). Also mediates the transport of urea across the cell membrane of the renal inner medullary collecting duct which is critical to the urinary concentrating mechanism (By similarity). Facilitates water transport in erythrocytes (By similarity).
See full target information SLC14A1
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