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AB153316

Recombinant Human SLC40A1 protein (GST tag N-Terminus)

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Recombinant Human SLC40A1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 571 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

FPN, FPN1, IREG1, SLC11A3, MSTP079, SLC40A1, Ferroportin, Ferroportin-1, Iron-regulated transporter 1, Solute carrier family 40 member 1

1 Images
SDS-PAGE - Recombinant Human SLC40A1 protein (GST tag N-Terminus) (AB153316)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SLC40A1 protein (GST tag N-Terminus) (AB153316)

ab153316 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q9NP59

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB78066

Anti-SLC40A1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC40A1 antibody (AB78066)

  • 5
  • 3
Primary Antibodies

AB75973

Anti-Ferritin heavy chain antibody [EPR3004Y]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (AB75973)

  • 4
  • 7
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB181153

Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MTRAGDHNRQRGCCGSLADYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLLTAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKHELLTMYHGWVLTSCYILIITIANIANLASTATAITIQRDWIVVVAGEDRSKLANMNATIRRIDQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYVLLWKVYQKTPALAVKAGLKEEETELKQLNLHKDTEPKPLEGTHLMGVKDSNIHELEHEQEPTCASQMAEPFRTFRDGWVSYYNQPVFLAGMGLAFLYMTVLGFDCITTGYAYTQGLSGSILSILMGASAITGIMGTVAFTWLRRKCGLVRTGLISGLAQLSCLILCVISVFMPGSPLDLSVSPFEDIRSRFIQGESITPTKIPEITTEIYMSNGSNSANIVPETSPESVPIISVSLLFAGVIAARIGLWSFDLTVTQLLQENVIESERGIINGVQNSMNYLLDLLHFIMVILAPNPEAFGLLVLISVSFVAMGHIMYFRFAQNTLGNKLFACGPDAKEVRKENQANTSVV","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":571,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9NP59","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLC40A1 also known as ferroportin or solute carrier family 40 member 1 is a transmembrane protein which functions as an iron transporter. This protein plays a role in exporting iron from cells into the bloodstream. The molecular weight of SLC40A1 is approximately 62 kDa. It is highly expressed in cells involved in iron storage and metabolism such as enterocytes in the duodenum and macrophages which process senescent red blood cells.
Biological function summary

The function of this transporter is important for iron homeostasis in human body. SLC40A1 helps balance iron levels by moving iron across intestinal cells into the plasma. It does not function as part of a larger protein complex but its activity is controlled by hepcidin a hormone that degrades SLC40A1 and regulates iron flow into the circulation.

Pathways

SLC40A1 is essential in the iron homeostasis pathway and the hepcidin regulation pathway. These pathways ensure iron supply matches body demands. It is closely related to hepcidin due to their interactive roles in this regulatory system. Hepcidin binds to SLC40A1 leading to its internalization and degradation which decreases iron export.

Mutations in SLC40A1 can lead to diseases like hemochromatosis characterized by excessive iron accumulation and anemia as iron transport becomes inefficient. Through these conditions SLC40A1 connects to hepcidin where disruption in their interaction contributes to disease pathology. Managing SLC40A1 activity is therefore important for maintaining proper iron levels and preventing related health issues.
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Specifications

Form

Liquid

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General info

Function

Transports Fe(2+) from the inside of a cell to the outside of the cell, playing a key role for maintaining systemic iron homeostasis (PubMed : 15692071, PubMed : 22178646, PubMed : 22682227, PubMed : 24304836, PubMed : 29237594, PubMed : 29599243, PubMed : 30247984). Transports iron from intestinal, splenic, hepatic cells, macrophages and erythrocytes into the blood to provide iron to other tissues (By similarity). Controls therefore dietary iron uptake, iron recycling by macrophages and erythrocytes, and release of iron stores in hepatocytes (By similarity). When iron is in excess in serum, circulating HAMP/hepcidin levels increase resulting in a degradation of SLC40A1, thus limiting the iron efflux to plasma (PubMed : 22682227, PubMed : 29237594, PubMed : 32814342).

Sequence similarities

Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily.

Post-translational modifications

Polyubiquitinated by RNF217; leading to proteasomal degradation (By similarity). Under conditions of high systemic iron levels, both the hormone peptide hepcidin/HAMP and holo(iron bound)-transferrin/TF induce the ubiquitination, internalization and proteasomal degradation of SLC40A1 to control iron release from cells (PubMed:22682227, PubMed:37277838).

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Product protocols

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Target data

Transports Fe(2+) from the inside of a cell to the outside of the cell, playing a key role for maintaining systemic iron homeostasis (PubMed : 15692071, PubMed : 22178646, PubMed : 22682227, PubMed : 24304836, PubMed : 29237594, PubMed : 29599243, PubMed : 30247984). Transports iron from intestinal, splenic, hepatic cells, macrophages and erythrocytes into the blood to provide iron to other tissues (By similarity). Controls therefore dietary iron uptake, iron recycling by macrophages and erythrocytes, and release of iron stores in hepatocytes (By similarity). When iron is in excess in serum, circulating HAMP/hepcidin levels increase resulting in a degradation of SLC40A1, thus limiting the iron efflux to plasma (PubMed : 22682227, PubMed : 29237594, PubMed : 32814342).
See full target information SLC40A1
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