Recombinant Human SLCO4C1 protein is a Human Full Length protein, in the 1 to 724 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M K S A K G I E N L A F V P S S P D I L R R L S A S P S Q I E V S A L S S D P Q R E N S Q P Q E L Q K P Q E P Q K S P E P S L P S A P P N V S E E K L R S L S L S E F E E G S Y G W R N F H P Q C L Q R C N T P G G F L L H Y C L L A V T Q G I V V N G L V N I S I S T V E K R Y E M K S S L T G L I S S S Y D I S F C L L S L F V S F F G E R G H K P R W L A F A A F M I G L G A L V F S L P Q F F S G E Y K L G S L F E D T C V T T R N S T S C T S S T S S L S N Y L Y V F I L G Q L L L G A G G T P L Y T L G T A F L D D S V P T H K S S L Y I G T G Y A M S I L G P A I G Y V L G G Q L L T I Y I D V A M G E S T D V T E D D P R W L G A W W I G F L L S W I F A W S L I I P F S C F P K H L P G T A E I Q A G K T S Q A H Q S N S N A D V K F G K S I K D F P A A L K N L M K N A V F M C L V L S T S S E A L I T T G F A T F L P K F I E N Q F G L T S S F A A T L G G A V L I P G A A L G Q I L G G F L V S K F R M T C K N T M K F A L F T S G V A L T L S F V F M Y A K C E N E P F A G V S E S Y N G T G E L G N L I A P C N A N C N C S R S Y Y Y P V C G D G V Q Y F S P C F A G C S N P V A H R K P K V Y Y N C S C I E R K T E I T S T A E T F G F E A K A G K C E T H C A K L P I F L C I F F I V I I F T F M A G T P I T V S I L R C V N H R Q R S L A L G I Q F M V L R L L G T I P G P I I F G F T I D S T C I L W D I N D C G I K G A C W I Y D N I K M A H M L V A I S V T C K V I T M F F N G F A I F L Y K P P P S A T D V S F H K E N A V V T N V L A E Q D L N K I V K E G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Mediates the transport of organic anions such as steroids (estrone 3-sulfate, chenodeoxycholate, glycocholate) and thyroid hormones (3,3',5-triiodo-L-thyronine (T3), L-thyroxine (T4)), in the kidney (PubMed:14993604, PubMed:19129463, PubMed:20610891). Capable of transporting cAMP and pharmacological substances such as digoxin, ouabain and methotrexate (PubMed:14993604). Transport is independent of sodium, chloride ion, and ATP (PubMed:14993604). Transport activity is stimulated by an acidic extracellular environment due to increased substrate affinity to the transporter (PubMed:19129463). The driving force for this transport activity is currently not known (By similarity). The role of hydrogencarbonate (HCO3(-), bicarbonate) as the probable counteranion that exchanges for organic anions is still not well defined (PubMed:19129463). Functions as an uptake transporter at the apical membrane, suggesting a role in renal reabsorption (By similarity). Involved in the renal secretion of the uremic toxin ADMA (N(omega),N(omega)-dimethyl-L-arginine or asymmetrical dimethylarginine), which is associated to cardiovascular events and mortality, and the structurally related amino acids L-arginine and L-homoarginine (a cardioprotective biomarker) (PubMed:30865704). Can act bidirectionally, suggesting a dual protective role of this transport protein; exporting L-homoarginine after being synthesized in proximal tubule cells, and mediating uptake of ADMA from the blood into proximal tubule cells where it is degraded by the enzyme dimethylarginine dimethylaminohydrolase 1 (DDAH1) (PubMed:30865704, PubMed:32642843). May be involved in sperm maturation by enabling directed movement of organic anions and compounds within or between cells (By similarity). This ion-transporting process is important to maintain the strict epididymal homeostasis necessary for sperm maturation (By similarity). May have a role in secretory functions since seminal vesicle epithelial cells are assumed to secrete proteins involved in decapacitation by modifying surface proteins to facilitate the acquisition of the ability to fertilize the egg (By similarity).
Alternative names
OATP4C1, OATPX, SLC21A20, SLCO4C1, Solute carrier organic anion transporter family member 4C1, OATP-H, Organic anion transporter M1, Organic anion transporting polypeptide 4C1, Solute carrier family 21 member 20, OATP-M1
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Recombinant Human SLCO4C1 protein is a Human Full Length protein, in the 1 to 724 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q6ZQN7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M K S A K G I E N L A F V P S S P D I L R R L S A S P S Q I E V S A L S S D P Q R E N S Q P Q E L Q K P Q E P Q K S P E P S L P S A P P N V S E E K L R S L S L S E F E E G S Y G W R N F H P Q C L Q R C N T P G G F L L H Y C L L A V T Q G I V V N G L V N I S I S T V E K R Y E M K S S L T G L I S S S Y D I S F C L L S L F V S F F G E R G H K P R W L A F A A F M I G L G A L V F S L P Q F F S G E Y K L G S L F E D T C V T T R N S T S C T S S T S S L S N Y L Y V F I L G Q L L L G A G G T P L Y T L G T A F L D D S V P T H K S S L Y I G T G Y A M S I L G P A I G Y V L G G Q L L T I Y I D V A M G E S T D V T E D D P R W L G A W W I G F L L S W I F A W S L I I P F S C F P K H L P G T A E I Q A G K T S Q A H Q S N S N A D V K F G K S I K D F P A A L K N L M K N A V F M C L V L S T S S E A L I T T G F A T F L P K F I E N Q F G L T S S F A A T L G G A V L I P G A A L G Q I L G G F L V S K F R M T C K N T M K F A L F T S G V A L T L S F V F M Y A K C E N E P F A G V S E S Y N G T G E L G N L I A P C N A N C N C S R S Y Y Y P V C G D G V Q Y F S P C F A G C S N P V A H R K P K V Y Y N C S C I E R K T E I T S T A E T F G F E A K A G K C E T H C A K L P I F L C I F F I V I I F T F M A G T P I T V S I L R C V N H R Q R S L A L G I Q F M V L R L L G T I P G P I I F G F T I D S T C I L W D I N D C G I K G A C W I Y D N I K M A H M L V A I S V T C K V I T M F F N G F A I F L Y K P P P S A T D V S F H K E N A V V T N V L A E Q D L N K I V K E G
- Accession
- Q6ZQN7
- Protein length
- Full Length
- Amino acids
- 1 to 724
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Mediates the transport of organic anions such as steroids (estrone 3-sulfate, chenodeoxycholate, glycocholate) and thyroid hormones (3,3',5-triiodo-L-thyronine (T3), L-thyroxine (T4)), in the kidney (PubMed:14993604, PubMed:19129463, PubMed:20610891). Capable of transporting cAMP and pharmacological substances such as digoxin, ouabain and methotrexate (PubMed:14993604). Transport is independent of sodium, chloride ion, and ATP (PubMed:14993604). Transport activity is stimulated by an acidic extracellular environment due to increased substrate affinity to the transporter (PubMed:19129463). The driving force for this transport activity is currently not known (By similarity). The role of hydrogencarbonate (HCO3(-), bicarbonate) as the probable counteranion that exchanges for organic anions is still not well defined (PubMed:19129463). Functions as an uptake transporter at the apical membrane, suggesting a role in renal reabsorption (By similarity). Involved in the renal secretion of the uremic toxin ADMA (N(omega),N(omega)-dimethyl-L-arginine or asymmetrical dimethylarginine), which is associated to cardiovascular events and mortality, and the structurally related amino acids L-arginine and L-homoarginine (a cardioprotective biomarker) (PubMed:30865704). Can act bidirectionally, suggesting a dual protective role of this transport protein; exporting L-homoarginine after being synthesized in proximal tubule cells, and mediating uptake of ADMA from the blood into proximal tubule cells where it is degraded by the enzyme dimethylarginine dimethylaminohydrolase 1 (DDAH1) (PubMed:30865704, PubMed:32642843). May be involved in sperm maturation by enabling directed movement of organic anions and compounds within or between cells (By similarity). This ion-transporting process is important to maintain the strict epididymal homeostasis necessary for sperm maturation (By similarity). May have a role in secretory functions since seminal vesicle epithelial cells are assumed to secrete proteins involved in decapacitation by modifying surface proteins to facilitate the acquisition of the ability to fertilize the egg (By similarity).
- Sequence similarities
Belongs to the organo anion transporter (TC 2.A.60) family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLCO4C1 also known as organic anion transporter 4C1 is part of the solute carrier organic anion transporter family. It functions in transporting organic anions across cell membranes. This protein facilitates the movement of drugs and endogenous molecules playing a role in pharmacokinetics and detoxification processes. It is around 80 kDa in size. SLCO4C1 is mainly expressed in the kidney and heart which indicates its influence on renal and cardiac function.
- Biological function summary
SLCO4C1 is involved in the cellular uptake and clearance of various organic compounds including toxins and drugs. It does not form part of any larger protein complexes but functions as an integral membrane transporter. This transport activity impacts cellular homeostasis and contributes to the regulation of substances in physiological environments balancing the distribution of substrates across membranes effectively.
- Pathways
SLCO4C1 participates in the organic anion transport pathway. This pathway is important in processes like drug metabolism and elimination. It interacts with other solute carrier family members to ensure effective transport of a variety of molecules. Proteins such as SLCO1B1 are related to SLCO4C1 through shared functions in this pathway impacting drug clearance and removal from the bloodstream.
- Associated diseases and disorders
SLCO4C1 plays a role in hypertension and chronic kidney disease. Alterations in SLCO4C1 expression or function can affect blood pressure regulation and kidney function due to its role in transporting signaling molecules and drugs that impact vascular health. It is also linked to the activity of transporters like SLCO2B1 which can modify the pharmacokinetics of medications influencing therapeutic outcomes and risks in these conditions.
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1 product image
SDS-PAGE - Recombinant Human SLCO4C1 protein (ab166349)
ab166349 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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