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AB164865

Recombinant Human SLFN11 protein (GST tag N-Terminus)

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Recombinant Human SLFN11 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 901 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

Schlafen family member 11, SLFN11

1 Images
SDS-PAGE - Recombinant Human SLFN11 protein (GST tag N-Terminus) (AB164865)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SLFN11 protein (GST tag N-Terminus) (AB164865)

ab164865 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q7Z7L1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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SDS-PAGE - Recombinant Human ZBTB46 protein (GST tag N-Terminus) (AB165467)

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Primary Antibodies

AB271354

Anti-SLFN11 antibody [EPR24414-87]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN11 antibody [EPR24414-87] (AB271354)

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Recombinant Human Vinculin protein (His tag)

SDS-PAGE - Recombinant Human Vinculin protein (His tag) (AB276192)

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Proteins & Peptides

AB165841

Recombinant Human UBR1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human UBR1 protein (GST tag N-Terminus) (AB165841)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MEANQCPLVVEPSYPDLVINVGEVTLGEENRKKLQKIQRDQEKERVMRAACALLNSGGGVIRMAKKVEHPVEMGLDLEQSLRELIQSSDLQAFFETKQQGRCFYIFVKSWSSGPFPEDRSVKPRLCSLSSSLYRRSETSVRSMDSREAFCFLKTKRKPKILEEGPFHKIHKGVYQELPNSDPADPNSDPADLIFQKDYLEYGEILPFPESQLVEFKQFSTKHFQEYVKRTIPEYVPAFANTGGGYLFIGVDDKSREVLGCAKENVDPDSLRRKIEQAIYKLPCVHFCQPQRPITFTLKIVDVLKRGELYGYACMIRVNPFCCAVFSEAPNSWIVEDKYVCSLTTEKWVGMMTDTDPDLLQLSEDFECQLSLSSGPPLSRPVYSKKGLEHKKELQQLLFSVPPGYLRYTPESLWRDLISEHRGLEELINKQMQPFFRGILIFSRSWAVDLNLQEKPGVICDALLIAQNSTPILYTILREQDAEGQDYCTRTAFTLKQKLVNMGGYTGKVCVRAKVLCLSPESSAEALEAAVSPMDYPASYSLAGTQHMEALLQSLVIVLLGFRSLLSDQLGCEVLNLLTAQQYEIFSRSLRKNRELFVHGLPGSGKTIMAMKIMEKIRNVFHCEAHRILYVCENQPLRNFISDRNICRAETRKTFLRENFEHIQHIVIDEAQNFRTEDGDWYGKAKSITRRAKGGPGILWIFLDYFQTSHLDCSGLPPLSDQYPREELTRIVRNADPIAKYLQKEMQVIRSNPSFNIPTGCLEVFPEAEWSQGVQGTLRIKKYLTVEQIMTCVADTCRRFFDRGYSPKDVAVLVSTAKEVEHYKYELLKAMRKKRVVQLSDACDMLGDHIVLDSVRRFSGLERSIVFGIHPRTADPAILPNVLICLASRAKQHLYIFPWGGH","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":901,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q7Z7L1","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLFN11 also known as Schlafen family member 11 is an important protein involved in the regulation of cellular response to DNA damage. With a molecular mass of approximately 104 kDa SLFN11 is extensively expressed in tumor cells and certain normal tissues particularly in the spleen thymus and testis. This protein contributes to the modulation of interferon-mediated immune responses and impacts translational control by tRNA activation playing a role in ribosomal assembly and function.
Biological function summary

SLFN11 functions to enhance the sensitivity of cells to DNA-damaging agents including chemotherapeutic drugs. It is important for impairing replication fork stability by inhibiting the expression of trans-translation machinery. SLFN11 serves as an important determinant within the DNA damage response (DDR) network and is known to interact with other DDR proteins emphasizing its impact on genome integrity and cellular survival under stress conditions.

Pathways

SLFN11 plays a notable role within the pathways associated with the DNA damage response and cell cycle regulation. The protein interacts notably with the ATR signaling pathway which is essential for sensing DNA damage. Additionally SLFN11 forms part of a complex interaction with other DNA repair proteins such as BRCA1 influencing the homologous recombination repair pathway contributing to the cellular determination of treatment response.

SLFN11 has significant implications in cancer particularly in the context of treatment response to chemotherapy. Cancers with high SLFN11 expression often exhibit increased sensitivity to cisplatin and other DNA-damaging agents pointing to its potential role as a biomarker for therapeutic efficacy. Moreover the protein is implicated in neurodegenerative disorders like amyotrophic lateral sclerosis (ALS) where its regulation could contribute to the pathophysiology. Its relationship with proteins such as p53 further highlights SLFN11's involvement in cancer and neuroprotection pathways.
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Specifications

Form

Liquid

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General info

Function

Inhibitor of DNA replication that promotes cell death in response to DNA damage (PubMed : 22927417, PubMed : 26658330, PubMed : 29395061). Acts as a guardian of the genome by killing cells with defective replication (PubMed : 29395061). Persistently blocks stressed replication forks by opening chromatin across replication initiation sites at stressed replication forks, possibly leading to unwind DNA ahead of the MCM helicase and block fork progression, ultimately leading to cell death (PubMed : 29395061). Upon DNA damage, inhibits translation of ATR or ATM based on distinct codon usage without disrupting early DNA damage response signaling (PubMed : 30374083). Antiviral restriction factor with manganese-dependent type II tRNA endoribonuclease (PubMed : 36115853). A single tRNA molecule is bound and cleaved by the SLFN11 dimer (PubMed : 36115853). Specifically abrogates the production of retroviruses such as human immunodeficiency virus 1 (HIV-1) by acting as a specific inhibitor of the synthesis of retroviruses encoded proteins in a codon-usage-dependent manner (PubMed : 23000900). Impairs the replication of human cytomegalovirus (HCMV) and some Flaviviruses (PubMed : 35105802, PubMed : 36115853). Exploits the unique viral codon bias towards A/T nucleotides (PubMed : 23000900). Also acts as an interferon (IFN)-induced antiviral protein which acts as an inhibitor of retrovirus protein synthesis (PubMed : 23000900).

Sequence similarities

Belongs to the Schlafen family. Subgroup III subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Inhibitor of DNA replication that promotes cell death in response to DNA damage (PubMed : 22927417, PubMed : 26658330, PubMed : 29395061). Acts as a guardian of the genome by killing cells with defective replication (PubMed : 29395061). Persistently blocks stressed replication forks by opening chromatin across replication initiation sites at stressed replication forks, possibly leading to unwind DNA ahead of the MCM helicase and block fork progression, ultimately leading to cell death (PubMed : 29395061). Upon DNA damage, inhibits translation of ATR or ATM based on distinct codon usage without disrupting early DNA damage response signaling (PubMed : 30374083). Antiviral restriction factor with manganese-dependent type II tRNA endoribonuclease (PubMed : 36115853). A single tRNA molecule is bound and cleaved by the SLFN11 dimer (PubMed : 36115853). Specifically abrogates the production of retroviruses such as human immunodeficiency virus 1 (HIV-1) by acting as a specific inhibitor of the synthesis of retroviruses encoded proteins in a codon-usage-dependent manner (PubMed : 23000900). Impairs the replication of human cytomegalovirus (HCMV) and some Flaviviruses (PubMed : 35105802, PubMed : 36115853). Exploits the unique viral codon bias towards A/T nucleotides (PubMed : 23000900). Also acts as an interferon (IFN)-induced antiviral protein which acts as an inhibitor of retrovirus protein synthesis (PubMed : 23000900).
See full target information SLFN11
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