Recombinant Human Slit2 Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.
>=80% SDS-PAGE
HEK 293 cells
His tag C-Terminus
SDS-PAGE, sELISA
No
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal cord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal cord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
SLIL3, SLIT2, SLIL3, Slit homolog 2 protein, Slit-2
Recombinant Human Slit2 Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.
>=80% SDS-PAGE
HEK 293 cells
His tag C-Terminus
SDS-PAGE, sELISA
No
Yes
Human
pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic
Fragment
46.6 kDa
Recombinant
His tag C-Terminus
Liquid
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal cord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal cord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
Dry Ice
-80°C
-80°C
Upon delivery aliquot
Avoid freeze / thaw cycle
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
This supplementary information is collated from multiple sources and compiled automatically.
Slit2 also known as Slit homolog 2 protein is a guidance cue protein with a mass of approximately 200 kDa. It plays a role in axonal guidance and cellular migration. Slit2 is expressed in the central nervous system where it contributes to neural development. It is also found in various tissues such as the liver lungs and kidneys. Slit2 mediates its effects primarily through interaction with Roundabout (Robo) receptors.
Slit2 functions in guiding axons during neuronal development and preventing them from straying from their pathways. It complexes with Robo receptors to transmit repulsive directional signals. This interaction is essential in the development of the nervous system ensuring proper neural circuit formation and synaptic connectivity. Besides neural guidance Slit2 also regulates other cellular events like angiogenesis and immune cell migration.
Slit2 participates in the axon guidance pathway where it influences neural network structuring by interacting with Robo receptors and other guidance cues. The Slit-Robo pathway plays an important role in axonal repulsion working alongside the Netrin signaling pathway that involves proteins like DCC and UNC5. This way Slit2 and its pathways balance attractive and repulsive cues to form functional neuronal networks.
Slit2 has associations with cancers particularly glioblastoma where it affects tumor cell migration and invasion. Dysregulation of the Slit-Robo signaling may lead to enhanced cancer cell metastasis. Additionally Slit2 is linked to congenital heart diseases where its malfunctioning affects cardiac development. Its interaction with Robo and other proteins like Ephrin can influence these disease processes suggesting a broader role in both developmental and pathological contexts.
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SDS-PAGE analysis of ab316436 under reducing conditions for 2ug protein.
Sandwich ELISA with the capture antibody dilution at 2 ug/mL and detector antibody dilution at 0.5 ug/mL.
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