Recombinant Human SLP76 protein (His tag) is a Human Full Length protein, in the 1 to 533 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A D P M A L R N V P F R S E V L G W D P D S L A D Y F K K L N Y K D C E K A V K K Y H I D G A R F L N L T E N D I Q K F P K L R V P I L S K L S Q E I N K N E E R R S I F T R K P Q V P R F P E E T E S H E E D N G G W S S F E E D D Y E S P N D D Q D G E D D G D Y E S P N E E E E A P V E D D A D Y E P P P S N D E E A L Q N S I L P A K P F P N S N S M Y I D R P P S G K T P Q Q P P V P P Q R P M A A L P P P P A G R N H S P L P P P Q T N H E E P S R S R N H K T A K L P A P S I D R S T K P P L D R S L A P F D R E P F T L G K K P P F S D K P S I P A G R S L G E H L P K I Q K P P L P P T T E R H E R S S P L P G K K P P V P K H G W G P D R R E N D E D D V H Q R P L P Q P A L L P M S S N T F P S R S T K P S P M N P L P S S H M P G A F S E S N S S F P Q S A S L P P Y F S Q G P S N R P P I R A E G R N F P L P L P N K P R P P S P A E E E N S L N E E W Y V S Y I T R P E A E A A L R K I N Q D G T F L V R D S S K K T T T N P Y V L M V L Y K D K V Y N I Q I R Y Q K E S Q V Y L L G T G L R G K E D F L S V S D I I D Y F R K M P L L L I D G K N R G S R Y Q C T L T H A A G Y P H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Adapter protein primarily involved in signaling pathways within T-cells, as well as other immune cells such as platelets, mast cells, and natural killer (NK) cells (PubMed:11313406, PubMed:33159816). Plays a crucial role for transducing signal from the T-cell receptor (TCR) after antigen recognition leading to T-cell activation. Mechanistically, once phosphorylated by the kinase ZAP70, mediates interactions with the guanine-nucleotide exchange factor VAV1, the adapter protein NCK and the kinase ITK (PubMed:8673706, PubMed:8702662). In turn, stimulates the activation of PKC-theta/PRKCQ and NF-kappa-B transcriptional activity in response to CD3 and CD28 costimulation (PubMed:11313406). Plays also an essential role in AGER-induced signaling pathways including p38 MAPK and ERK1/2 activation leading to cytokine release and pro-inflammatory responses (PubMed:33436632).
Alternative names
Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76, LCP2
Recommended products
Recombinant Human SLP76 protein (His tag) is a Human Full Length protein, in the 1 to 533 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q13094-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A D P M A L R N V P F R S E V L G W D P D S L A D Y F K K L N Y K D C E K A V K K Y H I D G A R F L N L T E N D I Q K F P K L R V P I L S K L S Q E I N K N E E R R S I F T R K P Q V P R F P E E T E S H E E D N G G W S S F E E D D Y E S P N D D Q D G E D D G D Y E S P N E E E E A P V E D D A D Y E P P P S N D E E A L Q N S I L P A K P F P N S N S M Y I D R P P S G K T P Q Q P P V P P Q R P M A A L P P P P A G R N H S P L P P P Q T N H E E P S R S R N H K T A K L P A P S I D R S T K P P L D R S L A P F D R E P F T L G K K P P F S D K P S I P A G R S L G E H L P K I Q K P P L P P T T E R H E R S S P L P G K K P P V P K H G W G P D R R E N D E D D V H Q R P L P Q P A L L P M S S N T F P S R S T K P S P M N P L P S S H M P G A F S E S N S S F P Q S A S L P P Y F S Q G P S N R P P I R A E G R N F P L P L P N K P R P P S P A E E E N S L N E E W Y V S Y I T R P E A E A A L R K I N Q D G T F L V R D S S K K T T T N P Y V L M V L Y K D K V Y N I Q I R Y Q K E S Q V Y L L G T G L R G K E D F L S V S D I I D Y F R K M P L L L I D G K N R G S R Y Q C T L T H A A G Y P H H H H H H
- Accession
- Q13094
- Protein length
- Full Length
- Predicted molecular weight
- 61.2 kDa
- Amino acids
- 1 to 533
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Adapter protein primarily involved in signaling pathways within T-cells, as well as other immune cells such as platelets, mast cells, and natural killer (NK) cells (PubMed:11313406, PubMed:33159816). Plays a crucial role for transducing signal from the T-cell receptor (TCR) after antigen recognition leading to T-cell activation. Mechanistically, once phosphorylated by the kinase ZAP70, mediates interactions with the guanine-nucleotide exchange factor VAV1, the adapter protein NCK and the kinase ITK (PubMed:8673706, PubMed:8702662). In turn, stimulates the activation of PKC-theta/PRKCQ and NF-kappa-B transcriptional activity in response to CD3 and CD28 costimulation (PubMed:11313406). Plays also an essential role in AGER-induced signaling pathways including p38 MAPK and ERK1/2 activation leading to cytokine release and pro-inflammatory responses (PubMed:33436632).
- Post-translational modifications
Phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2. SYK-dependent phosphorylation is required for recruitment of PI3K signaling components.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLP76 also known as Src homology 2 domain-containing leukocyte phosphoprotein of 76 kDa plays a pivotal role in intracellular signaling. This adaptor protein weighs approximately 76 kilodaltons and is predominantly expressed in hematopoietic cells particularly T cells NK cells mast cells and platelets. SLP76 functions to relay signals from the cell surface to the nucleus affecting cellular responses including activation proliferation and differentiation.
- Biological function summary
This adaptor protein serves as a scaffold linking membrane-proximal signaling to downstream cellular effectors. SLP76 forms part of a larger signaling complex with proteins such as LAT (Linker for Activation of T cells) and Gads facilitating the assembly of multi-protein complexes necessary for signal transduction. It coordinates the recruitment and activation of several enzymes and substrates which leads to robust immune cell responses.
- Pathways
The function of SLP76 integrates into T cell receptor (TCR) signaling and integrin signaling pathways. In TCR signaling SLP76 interacts closely with proteins LAT and ZAP-70 which are triggered upon antigen recognition. This interaction propagates the immune response by activating downstream effectors like MAP kinases and NF-kB. In integrin signaling SLP76 helps mediate cytoskeletal rearrangements which are important for cell migration and adhesion.
- Associated diseases and disorders
Mutations or dysregulation of SLP76 associate with immunodeficiencies and hematologic malignancies. Aberrant SLP76 activity can lead to inappropriate activation or inactivation of immune responses contributing to disorders as SCID (Severe Combined Immunodeficiency) and some types of leukemia. Additionally it connects with proteins such as LAT in disease contexts reflecting a shared pathway disruption contributing to the pathophysiology of these conditions.
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1 product image
SDS-PAGE - Recombinant Human SLP76 protein (His tag) (ab227404)
15% SDS-PAGE analysis of 3 μg ab227404.
MW: 70-100 kDa (SDS-PAGE under reducing conditions).
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