Recombinant Human SLUG protein is a Human Full Length protein, in the 1 to 268 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M P R S F L V K K H F N A S K K P N Y S E L D T H T V I I S P Y L Y E S Y S M P V I P Q P E I L S S G A Y S P I T V W T T A A P F H A Q L P N G L S P L S G Y S S S L G R V S P P P P S D T S S K D H S G S E S P I S D E E E R L Q S K L S D P H A I E A E K F Q C N L C N K T Y S T F S G L A K H K Q L H C D A Q S R K S F S C K Y C D K E Y V S L G A L K M H I R T H T L P C V C K I C G K A F S R P W L L Q G H I R T H T G E K P F S C P H C N R A F A D R S N L R A H L Q T H S D V K K Y Q C K N C S K T F S R M S L L H K H E E S G C C V A H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (By similarity). Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis.
Alternative names
SLUG, SLUGH, SNAI2, Zinc finger protein SNAI2, Neural crest transcription factor Slug, Protein snail homolog 2
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Recombinant Human SLUG protein is a Human Full Length protein, in the 1 to 268 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O43623-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M P R S F L V K K H F N A S K K P N Y S E L D T H T V I I S P Y L Y E S Y S M P V I P Q P E I L S S G A Y S P I T V W T T A A P F H A Q L P N G L S P L S G Y S S S L G R V S P P P P S D T S S K D H S G S E S P I S D E E E R L Q S K L S D P H A I E A E K F Q C N L C N K T Y S T F S G L A K H K Q L H C D A Q S R K S F S C K Y C D K E Y V S L G A L K M H I R T H T L P C V C K I C G K A F S R P W L L Q G H I R T H T G E K P F S C P H C N R A F A D R S N L R A H L Q T H S D V K K Y Q C K N C S K T F S R M S L L H K H E E S G C C V A H
- Accession
- O43623
- Protein length
- Full Length
- Predicted molecular weight
- 32.4 kDa
- Amino acids
- 1 to 268
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (By similarity). Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis.
- Sequence similarities
Belongs to the snail C2H2-type zinc-finger protein family.
- Post-translational modifications
Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for ubiquitination.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLUG also known as SNAI2 is a zinc finger transcription factor that plays a mechanical role in the regulation of genes implicated in cell differentiation and development. With an approximate mass of 29 kDa SLUG is expressed in various tissues particularly in the neural crest and epithelial-cell precursors. It acts as a repressor by binding to E-box motifs in the promoter regions of its target genes. SLUG functions in coordination with other cofactors to modulate gene expression that influences diverse biological processes including epithelial-mesenchymal transition (EMT).
- Biological function summary
SLUG influences cell motility and invasion by controlling EMT a process critical for development and cancer metastasis. SLUG functions as a part of the Snail family of transcription factors and collaborates with other EMT-related molecules. It influences the expression of genes that maintain the epithelial phenotype facilitating the switch to a mesenchymal state required for increased cell mobility. Through these actions SLUG participates in the dynamic remodeling of tissues and is an important player during embryonic development and in certain pathological conditions.
- Pathways
Researchers have associated SLUG with the TGF-beta and Wnt signaling pathways both essential for cell growth and differentiation. These pathways support SLUG's role in promoting EMT by modulating its transcriptional activity. SLUG often interacts with proteins such as TWIST1 and ZEB1 which synergistically act to downregulate epithelial markers and upregulate mesenchymal markers. This synergy highlights SLUG's significant role in facilitating changes in cellular architecture and function.
- Associated diseases and disorders
SLUG shows a strong connection with the progression of cancers and fibrosis. It contributes to cancer metastasis through its competence in inducing EMT enabling tumor cells to invade and establish secondary tumors. In breast cancer SLUG correlates with increased invasiveness and poor prognosis. In fibrosis SLUG promotes tissue scarring by facilitating fibroblast activation and extracellular matrix deposition. SLUG has interactions with other proteins such as E-Cadherin where its repressing activity on E-Cadherin contributes to the disruption of cell-cell adhesion a hallmark of metastatic cells.
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1 product image
SDS-PAGE - Recombinant Human SLUG protein (ab201878)
15% SDS Page analysis of ab201878 (3μg).
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