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AB140808

Recombinant Human SMAD9 protein (GST tag N-Terminus)

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Recombinant Human SMAD9 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 467 aa range, expressed in Escherichia coli, with >70%, suitable for SDS-PAGE, WB.
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SDS-PAGE - Recombinant Human SMAD9 protein (GST tag N-Terminus) (AB140808)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SMAD9 protein (GST tag N-Terminus) (AB140808)

SDS PAGE analysis of ab140808 at ~82 kDa.

Key facts

Purity

>70% Densitometry

Expression system

Escherichia coli

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

O15198-1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

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Complementary Products

Primary Antibodies

AB262940

Anti-SMAD9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD9 antibody (AB262940)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MHSTTPISSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDSLVKKLKKKKGAMDELERALSCPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYRRVETPVLPPVLVPRHSEYNPQLSLLAKFRSASLHSEPLMPHNATYPDSFQQPPCSALPPSPSHAFSQSPCTASYPHSPGSPSEPESPYQHSVDTPPLPYHATEASETQSGQPVDATADRHVVLSIPNGDFRPVCYEEPQHWCSVAYYELNNRVGETFQASSRSVLIDGFTDPSNNRNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECVSDSSIFVQSRNCNYQHGFHPATVCKIPSGCSLKVFNNQLFAQLLAQSVHHGFEVVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS","proteinLength":"Full Length","predictedMolecularWeight":"86 kDa","actualMolecularWeight":null,"aminoAcidEnd":467,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O15198","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMAD9 also known as Mothers against decapentaplegic homolog 9 or MADH9 is a transcription factor involved in signal transduction and transcriptional activity. It has a molecular mass of approximately 50 kDa. SMAD9 is widely expressed in various tissues including the heart brain and skeletal muscle. In its mechanical function SMAD9 becomes activated through phosphorylation by type I receptor serine/threonine kinases and forms part of the SMAD protein family that transmits extracellular signals to the nucleus affecting gene expression.
Biological function summary

SMAD9 plays a role in the cellular processes of growth and differentiation particularly within the bone morphogenetic protein (BMP) signaling pathway. It operates as part of a complex with other SMAD proteins such as SMAD1 and SMAD5 forming heteromeric complexes that regulate the transcription of BMP-responsive genes. This gene transcription is vital for the development and repair of tissues as well as the maintenance of cellular homeostasis.

Pathways

SMAD9 participates significantly in the BMP and transforming growth factor-beta (TGF-β) signaling pathways. These pathways are important for a variety of cellular responses including proliferation apoptosis and differentiation. SMAD9 associates with BMP receptors to mediate the BMP signaling cascade acting synergistically with other SMAD family members like SMAD4 which is a common mediator in these pathways. This interrelation places SMAD9 at a pivotal point within the regulation of multiple cellular responses.

Research indicates that SMAD9 mutations or dysregulation can associate with pulmonary arterial hypertension (PAH) and certain forms of cancer. In PAH altered SMAD9 signaling disrupts normal vascular remodeling resulting in elevated pulmonary artery pressure. Additionally it can interact with other proteins like BMPR2 a receptor for BMP which also plays a significant role in the pathogenesis of PAH. Understanding the involvement of SMAD9 in these conditions can provide insights into potential therapeutic targets for treatment.
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General info

Function

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).

Sequence similarities

Belongs to the dwarfin/SMAD family.

Post-translational modifications

Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
See full target information SMAD9

Alternative Names

MADH6, MADH9, SMAD8, SMAD9, SMAD family member 9, SMAD 9, Madh6, Mothers against decapentaplegic homolog 9, MAD homolog 9, Mothers against DPP homolog 9
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Product promise

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