Recombinant Human SMARCA2 / BRM protein (Tagged) is a Human Fragment protein, in the 700 to 1216 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
S Y Y T V A H A I S E R V E K Q S A L L I N G T L K H Y Q L Q G L E W M V S L Y N N N L N G I L A D E M G L G K T I Q T I A L I T Y L M E H K R L N G P Y L I I V P L S T L S N W T Y E F D K W A P S V V K I S Y K G T P A M R R S L V P Q L R S G K F N V L L T T Y E Y I I K D K H I L A K I R W K Y M I V D E G H R M K N H H C K L T Q V L N T H Y V A P R R I L L T G T P L Q N K L P E L W A L L N F L L P T I F K S C S T F E Q W F N A P F A M T G E R V D L N E E E T I L I I R R L H K V L R P F L L R R L K K E V E S Q L P E K V E Y V I K C D M S A L Q K I L Y R H M Q A K G I L L T D G S E K D K K G K G G A K T L M N T I M Q L R K I C N H P Y M F Q H I E E S F A E H L G Y S N G V I N G A E L Y R A S G K F E L L D R I L P K L R A T N H R V L L F C Q M T S L M T I M E D Y F A F R N F L Y L R L D G T T K S E D R A A L L K K F N E P G S Q Y F I F L L S T R A G G L G L N L Q A A D T V V I F D S D W N P H Q D L Q A Q D R A H R I G Q Q N E V R V L R L C T V N S V E E K I L A A A K Y K L N V D Q K V I Q A G M F D Q K S S S H E R R A F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
Alternative names
BAF190B, BRM, SNF2A, SNF2L2, SMARCA2, Probable global transcription activator SNF2L2, ATP-dependent helicase SMARCA2, BRG1-associated factor 190B, Protein brahma homolog, SNF2-alpha, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2, hBRM
Recommended products
Recombinant Human SMARCA2 / BRM protein (Tagged) is a Human Fragment protein, in the 700 to 1216 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P51531-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- S Y Y T V A H A I S E R V E K Q S A L L I N G T L K H Y Q L Q G L E W M V S L Y N N N L N G I L A D E M G L G K T I Q T I A L I T Y L M E H K R L N G P Y L I I V P L S T L S N W T Y E F D K W A P S V V K I S Y K G T P A M R R S L V P Q L R S G K F N V L L T T Y E Y I I K D K H I L A K I R W K Y M I V D E G H R M K N H H C K L T Q V L N T H Y V A P R R I L L T G T P L Q N K L P E L W A L L N F L L P T I F K S C S T F E Q W F N A P F A M T G E R V D L N E E E T I L I I R R L H K V L R P F L L R R L K K E V E S Q L P E K V E Y V I K C D M S A L Q K I L Y R H M Q A K G I L L T D G S E K D K K G K G G A K T L M N T I M Q L R K I C N H P Y M F Q H I E E S F A E H L G Y S N G V I N G A E L Y R A S G K F E L L D R I L P K L R A T N H R V L L F C Q M T S L M T I M E D Y F A F R N F L Y L R L D G T T K S E D R A A L L K K F N E P G S Q Y F I F L L S T R A G G L G L N L Q A A D T V V I F D S D W N P H Q D L Q A Q D R A H R I G Q Q N E V R V L R L C T V N S V E E K I L A A A K Y K L N V D Q K V I Q A G M F D Q K S S S H E R R A F
- Accession
- P51531
- Protein length
- Fragment
- Predicted molecular weight
- 75.7 kDa
- Amino acids
- 700 to 1216
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
- Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
- Post-translational modifications
During apoptosis, cleaved by cathepsin CTSG to produce a 160 kDa cleavage product which localizes to the cytosol.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SMARCA2 protein also known as BRM (Brahma-related gene 1) is a component of the SWI/SNF chromatin remodeling complex. This protein has a molecular mass of approximately 185 kDa. Scientists observe that SMARCA2/BRM is expressed in various tissues including the brain and liver. It acts as an ATPase which moves histone octamers in chromatin and facilitates access to DNA. SMARCA2/BRM plays an important role in transcriptional activation by modifying chromatin structure.
- Biological function summary
SMARCA2/BRM interacts with other subunits to form the SWI/SNF complex important for regulating gene expression. This complex helps control the transcription of specific genes by repositioning nucleosomes. SMARCA2/BRM modulates cell cycle progression and differentiation as it influences transcription factor access to DNA. Its activity is essential for normal cellular responses maintaining cell homeostasis and development.
- Pathways
SMARCA2/BRM participates significantly in the cell cycle and Wnt signaling pathways. In the cell cycle it cooperates with proteins like p53 to regulate genes involved in cell growth and apoptosis. In the Wnt pathway SMARCA2/BRM interacts with beta-catenin to influence cellular responses to Wnt signals. These pathways are critical for cell proliferation and maintaining cellular integrity and SMARCA2/BRM serves as a pivotal modulator within these systems.
- Associated diseases and disorders
SMARCA2/BRM has a connection to cancer and Coffin-Siris syndrome. Altered expression of SMARCA2/BRM has been observed in several cancers where it frequently interacts with other oncogenic proteins disrupting normal regulatory functions and promoting tumorigenesis. Meanwhile mutations in SMARCA2 can lead to Coffin-Siris syndrome a genetic disorder affecting development and it is connected with other proteins like SOX2 which are also involved in developmental pathways. Understanding SMARCA2's role may uncover insights into therapeutic targets for these conditions.
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1 product image
SDS-PAGE - Recombinant Human SMARCA2 / BRM protein (Tagged) (ab236192)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab236192.
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