Recombinant Human Smoothened protein
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Recombinant Human Smoothened protein is a Human Fragment protein, in the 653 to 787 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
View Alternative Names
SMOH, SMO, Protein smoothened, Protein Gx
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human Smoothened protein (AB114221)
ab114221 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Smoothened influences cellular processes by participating in the Hedgehog signaling pathway. It operates as part of a complex with the Patched (PTCH) receptor. When the Hedgehog ligand binds to PTCH Smoothened becomes activated. This activation leads to changes in gene expression that control cell growth and differentiation. Smoothened is critical in embryonic development tissue regeneration and stem cell maintenance.
Pathways
Smoothened interacts with essential components of the Hedgehog signaling pathway. This pathway includes proteins like PTCH and Gli transcription factors. Smoothened activation triggers the release of these Gli proteins from inhibitory complexes which then migrate to the nucleus to modulate the transcription of target genes. The Hedgehog pathway is important in regulating developmental processes and cellular responses.
Specifications
Form
Liquid
General info
Function
G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes (By similarity). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia (PubMed : 19592253). Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity).
Sequence similarities
Belongs to the G-protein coupled receptor Fz/Smo family.
Post-translational modifications
Phosphorylation by GRK kinases is required for interaction with protein kinase A catalytic subunit PRKACA.
Target data
Complementary Products
Product promise
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