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AB185389

Recombinant Human SMPDL3A protein

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Recombinant Human SMPDL3A protein is a Human Full Length protein, in the 23 to 453 aa range, expressed in Mammalian, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

ASML3A, SMPDL3A, Cyclic GMP-AMP phosphodiesterase SMPDL3A, Acid sphingomyelinase-like phosphodiesterase 3a, ASM-like phosphodiesterase 3a

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Mammalian

Tags

His tag C-Terminus

Applications

SDS-PAGE, HPLC

applications

Biologically active

No

Accession

Q92484

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in 3X PBS

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Dissolve the lyophilized protein in 3X PBS. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. After reconstitution aliquot and store at < -20°C.
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Sequence info

[{"sequence":"LPVAPAGGRNPPPAIGQFWHVTDLHLDPTYHITDDHTKVCASSKGANASNPGPFGDVLCDSPYQLILSAFDFIKNSGQEASFMIWTGDSPPHVPVPELSTDTVINVITNMTTTIQSLFPNLQVFPALGNHDYWPQDQLPVVTSKVYNAVANLWKPWLDEEAISTLRKGGFYSQKVTTNPNLRIISLNTNLYYGPNIMTLNKTDPANQFEWLESTLNNSQQNKEKVYIIAHVPVGYLPSSQNITAMREYYNEKLIDIFQKYSDVIAGQFYGHTHRDSIMVLSDKKGSPVNSLFVAPAVTPVKSVLEKQTNNPGIRLFQYDPRDYKLLDMLQYYLNLTEANLKGESIWKLEYILTQTYDIEDLQPESLYGLAKQFTILDSKQFIKYYNYFFVSYDSSVTCDKTCKAFQICAIMNLDNISYADCLKQLYIKHNYVDHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"50 kDa","actualMolecularWeight":null,"aminoAcidEnd":453,"aminoAcidStart":23,"nature":"Recombinant","expressionSystem":"Mammalian","accessionNumber":"Q92484","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
A few weeks
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMPDL3A also known as sphingomyelin phosphodiesterase acid-like 3A is an enzyme with significant roles in lipid metabolism. It weighs around 47 kDa and primarily breaks down sphingomyelins into phosphocholine and ceramides. SMPDL3A shows expression in numerous tissues including the immune system and kidney cells where it aids cellular functions. It is localized primarily on the membranes and it is also found at increasing levels in pathological conditions.
Biological function summary

The enzyme SMPDL3A significantly impacts cellular lipid regulation and membrane dynamics. It does not typically function as part of a complex but stands out in remodeling membrane-bound lipids. SMPDL3A's ability to degrade sphingomyelins affects signaling pathways by altering ceramide levels influencing cellular response processes like apoptosis and proliferation. Therefore its biological role involves influencing membrane composition and cell signaling pathways that respond to various stimuli.

Pathways

This protein significantly participates in the regulation of the lipid metabolism and sphingolipid signaling pathways. It operates alongside other proteins like ceramidase and sphingosine kinase 1. SMPDL3A alters sphingolipid metabolism which influences the MAPK signaling pathway a pathway critical in cell growth and stress responses. By modulating ceramide levels it indirectly affects downstream interactions and protein activities related to these pathways.

Researchers associate SMPDL3A with conditions like diabetic nephropathy and systemic lupus erythematosus (SLE). In diabetic nephropathy SMPDL3A appears to interfere with kidney cellular responses while altered expression levels are noted in pathological states. In SLE SMPDL3A's expression changes link to immune cell dysfunction. The protein also interacts with others involved in these diseases such as CD95/Fas which influences apoptosis pathways further implicating its role in cellular health and disease progression.
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Specifications

Form

Lyophilized

Additional notes

Determined by SEC-HPLC and reducing SDS-PAGE.

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General info

Function

Cyclic-nucleotide phosphodiesterase that acts as a negative regulator of innate immunity by mediating degradation of 2',3'-cGAMP, thereby inhibiting the cGAS-STING signaling (PubMed : 37890481). Specifically linearizes 2',3'-cGAMP into 2'5'-bond pGpA and further hydrolyzes pGpA to produce GpA (PubMed : 37890481). Also has in vitro nucleotide phosphodiesterase activity with nucleoside triphosphates, such as ATP (PubMed : 25288789, PubMed : 26783088). Has in vitro activity with p-nitrophenyl-TMP (PubMed : 25288789). Has lower activity with nucleoside diphosphates, and no activity with nucleoside monophosphates (PubMed : 25288789, PubMed : 26783088, PubMed : 28104755). Has in vitro activity with CDP-choline, giving rise to CMP and phosphocholine. Has in vitro activity with CDP-ethanolamine (PubMed : 26783088). Does not have sphingomyelin phosphodiesterase activity (PubMed : 25288789, PubMed : 26783088).

Sequence similarities

Belongs to the acid sphingomyelinase family.

Post-translational modifications

N-glycosylation is required for protein maturation, secretion and phosphodiesterase activity.

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Product protocols

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Target data

Cyclic-nucleotide phosphodiesterase that acts as a negative regulator of innate immunity by mediating degradation of 2',3'-cGAMP, thereby inhibiting the cGAS-STING signaling (PubMed : 37890481). Specifically linearizes 2',3'-cGAMP into 2'5'-bond pGpA and further hydrolyzes pGpA to produce GpA (PubMed : 37890481). Also has in vitro nucleotide phosphodiesterase activity with nucleoside triphosphates, such as ATP (PubMed : 25288789, PubMed : 26783088). Has in vitro activity with p-nitrophenyl-TMP (PubMed : 25288789). Has lower activity with nucleoside diphosphates, and no activity with nucleoside monophosphates (PubMed : 25288789, PubMed : 26783088, PubMed : 28104755). Has in vitro activity with CDP-choline, giving rise to CMP and phosphocholine. Has in vitro activity with CDP-ethanolamine (PubMed : 26783088). Does not have sphingomyelin phosphodiesterase activity (PubMed : 25288789, PubMed : 26783088).
See full target information SMPDL3A
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