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AB162135

Recombinant Human SMPDL3B protein (GST tag N-Terminus)

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Recombinant Human SMPDL3B protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 373 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

ASML3B, ASMLPD, SMPDL3B, Acid sphingomyelinase-like phosphodiesterase 3b, ASM-like phosphodiesterase 3b

1 Images
SDS-PAGE - Recombinant Human SMPDL3B protein (GST tag N-Terminus) (AB162135)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SMPDL3B protein (GST tag N-Terminus) (AB162135)

ab162135 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q92485

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Secondary Antibodies

AB102448

Goat Anti-Mouse IgG+IgM+IgA H&L (HRP)

  • 0
  • 0
Secondary Antibodies

AB125912

Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L

RabMAb|Recombinant

ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (AB125912)

  • 0
  • 0
Secondary Antibodies

AB9159

Goat Anti-Human IgE epsilon chain

  • 0
  • 0
Proteins & Peptides

AB277009

Recombinant Mouse OSTM1 protein (His tag)

SDS-PAGE - Recombinant Mouse OSTM1 protein (His tag) (AB277009)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRLLAWLIFLANWGGARAEPGKFWHIADLHLDPDYKVSKDPFQVCPSAGSQPVPDAGPWGDYLCDSPWALINSSIYAMKEIEPEPDFILWTGDDTPHVPDEKLGEAAVLEIVERLTKLIREVFPDTKVYAALGNHDFHPKNQFPAGSNNIYNQIAELWKPWLSNESIALFKKGAFYCEKLPGPSGAGRIVVLNTNLYYTSNALTADMADPGQQFQWLEDVLTDASKAGDMVYIVGHVPPGFFEKTQNKAWFREGFNEKYLKVVRKHHRVIAGQFFGHHHTDSFRMLYDDAGVPISAMFITPGVTPWKTTLPGVVNGANNPAIRVFEYDRATLSLKVRSPAEARGGGWEGLKCITTFPHSQLIHLPLTTEPQEG","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":373,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q92485","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMPDL3B also known as sphingomyelin phosphodiesterase-like 3B is an enzyme with a mass of approximately 50 kDa. This protein belongs to the acid sphingomyelinase family and is primarily involved in the hydrolysis of sphingomyelin to ceramide and phosphorylcholine. SMPDL3B is expressed in several tissues including the liver kidney and immune cells where it plays an important role in maintaining cellular sphingolipid metabolism.
Biological function summary

SMPDL3B regulates various cellular processes such as apoptosis proliferation and differentiation by controlling ceramide levels. It does not typically function as part of a larger protein complex but interacts with membrane components to exert its enzymatic activity. SMPDL3B modulates signal transduction pathways that are sensitive to changes in membrane lipid composition influencing cellular responses to stress and immune signals.

Pathways

SMPDL3B is involved in the sphingolipid metabolism pathway and the cholesterol metabolism pathway. It interacts with other sphingolipid enzymes like acid ceramidase which further regulates ceramide levels. SMPDL3B affects the activation of signaling proteins like protein kinase C which links it to various cellular responses. Changes in sphingolipid metabolism can influence pathways linked to cell survival and inflammation.

SMPDL3B plays a role in conditions such as cancer and atherosclerosis. Altered SMPDL3B expression or activity can lead to imbalances in ceramide levels contributing to the pathogenesis of these diseases. In cancer SMPDL3B influences tumor progression through its impact on cell growth and apoptosis interacting with proteins such as Bcl-2. In atherosclerosis it affects lipid accumulation and inflammation relating to proteins like low-density lipoprotein (LDL).
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Specifications

Form

Liquid

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General info

Function

Lipid-modulating phosphodiesterase (PubMed : 26095358). Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling (By similarity). Has in vitro phosphodiesterase activity, but the physiological substrate is unknown (PubMed : 26095358). Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro) (By similarity).

Sequence similarities

Belongs to the acid sphingomyelinase family.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

Lipid-modulating phosphodiesterase (PubMed : 26095358). Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling (By similarity). Has in vitro phosphodiesterase activity, but the physiological substrate is unknown (PubMed : 26095358). Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro) (By similarity).
See full target information SMPDL3B
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