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AB95930

Recombinant human SMURF 2 protein (DDDDK tag N-Terminus)

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Recombinant human SMURF 2 protein (DDDDK tag N-Terminus) is a Human Fragment protein, in the 130 to 748 aa range, expressed in Baculovirus infected insect cells, with >80%, suitable for SDS-PAGE, FuncS.

View Alternative Names

E3 ubiquitin-protein ligase SMURF2, hSMURF2, HECT-type E3 ubiquitin transferase SMURF2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMURF2

2 Images
Functional Studies - Recombinant human SMURF 2 protein (DDDDK tag N-Terminus) (AB95930)
  • FuncS

Unknown

Functional Studies - Recombinant human SMURF 2 protein (DDDDK tag N-Terminus) (AB95930)

Image showing specific activity of ab95930.

SDS-PAGE - Recombinant human SMURF 2 protein (DDDDK tag N-Terminus) (AB95930)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human SMURF 2 protein (DDDDK tag N-Terminus) (AB95930)

10% SDS-PAGE
Lane 1 : ab95930 (4μg)
Lane 2 : Molecular weight markers

Key facts

Purity

>80% SDS-PAGE

Expression system

Baculovirus infected insect cells

Tags

DDDDK tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 0.00049 pmol/min/μg Assay conditions: 50 μl reaction mix (4 mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5 μg), E2 (2.8 μg), Biotin-Ubiquitin (0.5 μg) and SMURF 2 (0-2 μg) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against SMURF 2, incubate 1h. Finally, incubate 1h with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm). Specific Activity: 0.00049 pmol/min/μg Assay conditions: 50 μl reaction mix (4 mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5 μg), E2 (2.8 μg), Biotin-Ubiquitin (0.5 μg) and SMURF 2 (0-2 μg) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against SMURF 2, incubate 1h. Finally, incubate 1h with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm).

Accession

Q9HAU4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Recombinant human XIAP protein (DDDDK tag N-Terminus)

Functional Studies - Recombinant human XIAP protein (DDDDK tag N-Terminus) (AB95933)

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Primary Antibodies

AB53316

Anti-SMURF 2 antibody [EP629Y3]

RabMAb|Recombinant|KO Validated

Western blot - Anti-SMURF 2 antibody [EP629Y3] (AB53316)

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AB112436

Recombinant Human Dub3 protein (GST tag N-Terminus)

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Primary Antibodies

AB154817

Anti-UGPase antibody [EPR10627(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] (AB154817)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"72 kDa","actualMolecularWeight":null,"aminoAcidEnd":748,"aminoAcidStart":130,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9HAU4","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMURF2 also known as SMAD specific E3 ubiquitin protein ligase 2 functions as an E3 ubiquitin ligase involved in protein ubiquitination. It weighs approximately 85 kDa and is part of the NEDD4 family of E3 ligases. SMURF2 facilitates the transfer of ubiquitin from an E2 conjugating enzyme to specific substrate proteins marking them for degradation via the proteasome pathway. This protein is widely expressed in various tissues including brain kidney and liver indicating its essential role in different cellular processes.
Biological function summary

SMURF2 regulates many cell signaling pathways including the TGF-beta/Smad pathway. It associates with SMAD proteins to control their stability and activity acting as a negative regulator to modulate TGF-beta signaling. By promoting the ubiquitination and degradation of receptor-regulated SMADs (R-SMADs) SMURF2 impacts cellular responses to growth factors. This regulatory mechanism is important for maintaining cellular proliferation differentiation and apoptosis.

Pathways

The function of SMURF2 significantly influences the TGF-beta and BMP signaling pathways both critical in controlling cell growth and differentiation. These pathways involve interactions with other proteins like SMAD7 and SMAD2. SMURF2 interacts with SMAD7 an inhibitory SMAD to regulate Smad-dependent transcriptional responses ensuring the fine-tuning of signal transduction. These pathways' balance and regulation are vital for appropriate cellular function and response.

SMURF2 has connections to cancer and fibrosis. Aberrant regulation of SMURF2 contributes to tumorigenesis where its altered expression levels can lead to unrestrained cell growth and cancer progression particularly in breast and prostate cancers. Additionally SMURF2 is involved in fibrosis with its dysregulation affecting extracellular matrix deposition. In both cancer and fibrosis SMURF2 interacts with TGF-beta and other related proteins highlighting its involvement in disease progression and sustaining pathological states.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 11016919, PubMed : 38016474). Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby down-regulating TGF-beta signaling (PubMed : 11163210, PubMed : 12717440, PubMed : 21791611). In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with AIMP1 (PubMed : 18448069). Also forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2 and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasome-mediated degradation (PubMed : 11016919, PubMed : 11158580, PubMed : 11389444). SMAD2 may recruit substrates, such as SNON, for ubiquitin-dependent degradation (PubMed : 11389444). Negatively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation (PubMed : 30696809). Acts as an activator of ferroptosis by mediating ubiquitination and degradation of GSTP1, thereby preventing detoxification of 4-hydroxynonenal (4-HNE) reactive aldehyde (PubMed : 38016474).. (Microbial infection) In case of filoviruses Ebola/EBOV and Marburg/MARV infection, the complex formed by viral matrix protein VP40 and SMURF2 facilitates virus budding.

Post-translational modifications

Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1 (PubMed:19343052, PubMed:30696809). Ubiquitinated by the SCF(FBXL15) complex and TTC3, leading to its degradation by the proteasome (PubMed:21572392, PubMed:30696809). 'Lys-48'-linked polyubiquitination mediated by TRAF4 at Lys-119 leads to SMURF2 proteasomal degradation (PubMed:31076633).

Subcellular localisation

Nucleus

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Product protocols

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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 11016919, PubMed : 38016474). Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby down-regulating TGF-beta signaling (PubMed : 11163210, PubMed : 12717440, PubMed : 21791611). In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with AIMP1 (PubMed : 18448069). Also forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2 and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasome-mediated degradation (PubMed : 11016919, PubMed : 11158580, PubMed : 11389444). SMAD2 may recruit substrates, such as SNON, for ubiquitin-dependent degradation (PubMed : 11389444). Negatively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation (PubMed : 30696809). Acts as an activator of ferroptosis by mediating ubiquitination and degradation of GSTP1, thereby preventing detoxification of 4-hydroxynonenal (4-HNE) reactive aldehyde (PubMed : 38016474).. (Microbial infection) In case of filoviruses Ebola/EBOV and Marburg/MARV infection, the complex formed by viral matrix protein VP40 and SMURF2 facilitates virus budding.
See full target information SMURF2
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