Recombinant human SMYD4 protein (Active)

Key facts

Purity

>=90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Biological activity

Assay Conditions: Add 50 μl reaction mix (100 M S-adenosylmethionine, SMYD4 enzyme, and HMT buffer 7Z') to wells coated with the substrate. Incubate 3-16 hours. Add antibody against methylated K4 residue of histone H3, incubate 1 hour. Then, add secondary HRP-labeled antibody and incubate 30 minutes. Finally, add HRP chemiluminescent substrates and read luminescence.

Accession

Q8IYR2-1

Animal free

No

Species

Human

Concentration

Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride, 0.01% TCEP HCl

Sequence info

Amino acid sequence

D L P V D E W K S Y L L Q K W A S L P T S V Q V T I S T A E T L R D I F L H S S S L L Q P E D E L F L K R L S K G Y L V G K D S D A P L F Y R E E G N K K F Q E K D Y T G A A V L Y S K G V S H S R P N T E D M S L C H A N R S A A L F H L G Q Y E T C L K D I N R A Q T H G Y P E R L Q P K I M L R K A E C L V A L G R L Q E A S Q T I S D L E R N F T A T P A L A D V L P Q T L Q R N L H R L K M K M Q E K D S L T E S F P A A L A K T L E D A A L R E E N E Q L S N A S S S I G L C V D P L K G R C L V A T K D I L P G E L L V Q E D A F V S V L N P G E L P P P H H G L D S K W D T R V T N G D L Y C H R C L K H T L A T V P C D G C S Y A K Y C S Q E C L Q Q A W E L Y H R T E C P L G G L L L T L G V F C H I A L R L T L L V G F E D V R K I I T K L C D K I S N K D I C L P E S N N Q V K T L N Y G L G E S E K N G N I V E T P I P G C D I N G K Y E N N Y N A V F N L L P H T E N H S P E H K F L C A L C V S A L C R Q L E A A S L Q A I P T E R I V N S S Q L K A A V T P E L C P D V T I W G V A M L R H M L Q L Q C N A Q A M T T I Q H T G P K G S I V T D S R Q V R L A T G I F P V I S L L N H S C S P N T S V S F I S T V A T I R A S Q R I R K G Q E I L H C Y G P H K S R M G V A E R Q Q K L R S Q Y F F D C A C P A C Q T E A H R M A A G P R W E A F C C N S C G A P M Q G D D V L R C G S R S C A E S A V S R D H L V S R L Q D L Q Q Q V R V A Q K L L R D G E L E R A V Q R L S G C Q R D A E S F L W A E H A V V G E I A D G L A R A C A A L G D W Q K S A T H L Q R S L Y V V E V R H G P S S V E M G H E L F K L A Q I F F N G F A V P E A L S T I Q K A E E V L S L H C G P W D D E I Q E L Q K M K S C L L D L P P T P V G P A L

Accession

Q8IYR2

Protein length

Full Length

Predicted molecular weight

90 kDa

Amino acids

2 to 804

Nature

Recombinant

Tags

DDDDK tag N-Terminus

Product promise