Recombinant Human SNAP25 protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, WB.
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Publications
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- Journal of Alzheimer's disease : JAD 59:1123-11372017Effect of APOE Genotype on Synaptic Proteins in Earlier Adult Life.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLindsey I Sinclair,Seth LovePubMed 28731446
- The Journal of biological chemistry 287:33607-142012Structural framework for covalent inhibition of Clostridium botulinum neurotoxin A by targeting Cys165.Applications:SDS-PAGEReactive species:Unspecified reactive speciesEnrico A Stura,Laura Le Roux,Karine Guitot,Sandra Garcia,Sarah Bregant,Fabrice Beau,Laura Vera,Guillaume Collet,Denis Ptchelkine,Huseyin Bakirci,Vincent DivePubMed 22869371
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M A E D A D M R N E L E E M Q R R A D Q L A D E S L E S T R R M L Q L V E E S K D A G I R T L V M L D E Q G E Q L E R I E E G M D Q I N K D M K E A E K N L T D L G K F C G L C V C P C N K L K S S D A Y K K A W G N N Q D G V V A S Q P A R V V D E R E Q M A I S G G F I R R V T N D A R E N E M D E N L E Q V S G I I G N L R H M A L D M G N E I D T Q N R Q I D R I M E K A D S N K T R I D E A N Q R A T K M L G S G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab74529 can be used as a WB positive control in conjunction with Anti-SNAP25 antibody ab41455. |
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Target data
Function
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Alternative names
SNAP, SNAP25, Synaptosomal-associated protein 25, SNAP-25, Super protein, Synaptosomal-associated 25 kDa protein, SUP
Recommended products
Recombinant Human SNAP25 protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P60880-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 10% Glycerol (glycerin, glycerine), 0.3025% Tris, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M A E D A D M R N E L E E M Q R R A D Q L A D E S L E S T R R M L Q L V E E S K D A G I R T L V M L D E Q G E Q L E R I E E G M D Q I N K D M K E A E K N L T D L G K F C G L C V C P C N K L K S S D A Y K K A W G N N Q D G V V A S Q P A R V V D E R E Q M A I S G G F I R R V T N D A R E N E M D E N L E Q V S G I I G N L R H M A L D M G N E I D T Q N R Q I D R I M E K A D S N K T R I D E A N Q R A T K M L G S G
- Accession
- P60880
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab74529 was purified by using conventional chromatography techniques.
General info
- Function
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
- Sequence similarities
Belongs to the SNAP-25 family.
- Post-translational modifications
Palmitoylated (PubMed:28757145). Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SNAP25 also known as Synaptosomal-associated protein 25 plays a critical role in neurotransmitter release through its involvement in the vesicle fusion process. It is part of the SNARE complex and is essential for the docking and fusion of synaptic vesicles with the presynaptic membrane. The protein is approximately 25 kDa in mass and is expressed abundantly in neurons within the central nervous system. Among its various synonyms you may encounter 'protein SNAP' in some literature.
- Biological function summary
SNARE proteins mediate the fusion of transport vesicles with their target membranes. SNAP25 as part of this complex facilitates synaptic-vesicle exocytosis by forming a tight complex with syntaxin and VAMP (also called synaptobrevin). This interaction promotes the merging of vesicle and plasma membranes enabling efficient neurotransmitter release into the synaptic cleft. SNAP25 undergoes dynamic regulation and modification which is important for precise synaptic function.
- Pathways
One observes SNAP25's integration in the process of neurotransmitter release specifically in the exocytosis pathway. It associates closely with other SNARE proteins such as syntaxin-1 and VAMP-2 embodying a core element in synaptic signaling mechanisms. The proper functioning of SNAP25 within this pathway assures the controlled release of neurotransmitters influencing synaptic plasticity and communication.
- Associated diseases and disorders
SNAP25 exhibits strong associations with conditions such as Attention Deficit Hyperactivity Disorder (ADHD) and epilepsy. Variants and dysregulation in SNAP25 have been linked to problems in synaptic transmission which can contribute to these neurological disorders. The protein also interacts with other SNARE complex proteins such as syntaxin which can potentially mediate its role in disease pathogenesis. Understanding these interactions offers insights into therapeutic targets for neurological disorders involving SNAP25.
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2 product images
Western blot - Recombinant Human SNAP25 protein (ab74529)
All lanes: Western blot - Anti-SNAP25 antibody (Anti-SNAP25 antibody ab41455) at 1 µg/mL
All lanes: Western blot - Recombinant Human SNAP25 protein (ab74529) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Exposure time: 1min
SDS-PAGE - Recombinant Human SNAP25 protein (ab74529)
15% SDS-PAGE showing ab74529 (3μg)
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