AB74529

Recombinant Human SNAP25 protein

Name: Recombinant Human SNAP25 protein (ab74529) | Abcam
Brand: Abcam Limited
SKU: AB74529
Price: 290 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Recombinant Human SNAP25 protein is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.

View Alternative Names

SNAP, SNAP25, Synaptosomal-associated protein 25, SNAP-25, Super protein, Synaptosomal-associated 25 kDa protein, SUP

2 Images

Unknown

Western blot - Recombinant Human SNAP25 protein (AB74529)

All lanes:

Western blot - Anti-SNAP25 antibody (<a href='/en-us/products/primary-antibodies/snap25-antibody-ab41455'>ab41455</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human SNAP25 protein (ab74529) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 23 kDa

true

Exposure time: 1min

SDS-PAGE - Recombinant Human SNAP25 protein (AB74529)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SNAP25 protein (AB74529)

15% SDS-PAGE showing ab74529 (3μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

WB, SDS-PAGE

applications

Biologically active

Accession

P60880

Animal free

Carrier free

Species

Human

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.3025% Tris, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab74529 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/snap25-antibody-ab41455'>ab41455</a>.</p>" } } }

style

left-column

style

left-column

Sequence info

[{"sequence":"MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P60880","tags":[]}]

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

False

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNAP25 also known as Synaptosomal-associated protein 25 plays a critical role in neurotransmitter release through its involvement in the vesicle fusion process. It is part of the SNARE complex and is essential for the docking and fusion of synaptic vesicles with the presynaptic membrane. The protein is approximately 25 kDa in mass and is expressed abundantly in neurons within the central nervous system. Among its various synonyms you may encounter 'protein SNAP' in some literature.

Biological function summary

SNARE proteins mediate the fusion of transport vesicles with their target membranes. SNAP25 as part of this complex facilitates synaptic-vesicle exocytosis by forming a tight complex with syntaxin and VAMP (also called synaptobrevin). This interaction promotes the merging of vesicle and plasma membranes enabling efficient neurotransmitter release into the synaptic cleft. SNAP25 undergoes dynamic regulation and modification which is important for precise synaptic function.

Pathways

One observes SNAP25's integration in the process of neurotransmitter release specifically in the exocytosis pathway. It associates closely with other SNARE proteins such as syntaxin-1 and VAMP-2 embodying a core element in synaptic signaling mechanisms. The proper functioning of SNAP25 within this pathway assures the controlled release of neurotransmitters influencing synaptic plasticity and communication.

SNAP25 exhibits strong associations with conditions such as Attention Deficit Hyperactivity Disorder (ADHD) and epilepsy. Variants and dysregulation in SNAP25 have been linked to problems in synaptic transmission which can contribute to these neurological disorders. The protein also interacts with other SNARE complex proteins such as syntaxin which can potentially mediate its role in disease pathogenesis. Understanding these interactions offers insights into therapeutic targets for neurological disorders involving SNAP25.

style

left-column

Specifications

Form

Liquid

Additional notes

ab74529 was purified by using conventional chromatography techniques.

style

left-column

General info

Function

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.

Sequence similarities

Belongs to the SNAP-25 family.

Post-translational modifications

Palmitoylated (PubMed:28757145). Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type A (BoNT/A, botA) which hydrolyzes the 197-Gln-|-Arg-198 bond and inhibits neurotransmitter release (PubMed:15592454, PubMed:9886085).. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type C (BoNT/C) which hydrolyzes the 198-Arg-|-Ala-199 bond and inhibits neurotransmitter release (PubMed:17718519, PubMed:9886085). C.botulinum type C only rarely infects humans.. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type E (BoNT/E) which hydrolyzes the 180-Arg-|-Ile-181 bond and inhibits neurotransmitter release (PubMed:9886085).

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information SNAP25

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of Alzheimer's disease : JAD 59:1123-1137 PubMed28731446

2017

Effect of APOE Genotype on Synaptic Proteins in Earlier Adult Life.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsey I Sinclair,Seth Love

The Journal of biological chemistry 287:33607-14 PubMed22869371

2012

Structural framework for covalent inhibition of Clostridium botulinum neurotoxin A by targeting Cys165.

Applications

SDS-PAGE

Species

Unspecified reactive species

Enrico A Stura,Laura Le Roux,Karine Guitot,Sandra Garcia,Sarah Bregant,Fabrice Beau,Laura Vera,Guillaume Collet,Denis Ptchelkine,Huseyin Bakirci,Vincent Dive

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant Human SNAP25 protein

Western blot - Recombinant Human SNAP25 protein (AB74529)

SDS-PAGE - Recombinant Human SNAP25 protein (AB74529)

Key facts

Purity

Expression system

Tags

Applications

Biologically active

Accession

Animal free

Carrier free

Species

Storage buffer

Reactivity data

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Specifications

Form

Additional notes

General info

Function

Sequence similarities

Post-translational modifications

Product protocols

Target data

Publications (2)

Complementary Products

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Product promise