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AB117001

Recombinant Human SNRPB/SmB protein

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Recombinant Human SNRPB/SmB protein is a Human Fragment protein, in the 1 to 80 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

COD, SNRPB1, SNRPB, Small nuclear ribonucleoprotein-associated proteins B and B', snRNP-B, Sm protein B/B', Sm-B/B', SmB/B'

1 Images
SDS-PAGE - Recombinant Human SNRPB/SmB protein (AB117001)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SNRPB/SmB protein (AB117001)

12.5% SDS-PAGE showing ab117001 at approx. 34.43 kDa.
Stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

P14678

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Recombinant protein.</p>" } } }
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Product details

This product was previously labelled as SNRPB.
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Sequence info

[{"sequence":"MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAEREEKRVLGLVLLRGENLVSM","proteinLength":"Fragment","predictedMolecularWeight":"34.43 kDa","actualMolecularWeight":null,"aminoAcidEnd":80,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P14678","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNRPB also known as SmB is a core component of the spliceosomal small nuclear ribonucleoproteins (snRNPs). It has a molecular weight of approximately 25 kDa. This protein is widely expressed in human tissues especially in those with high proliferative capacity like the brain and immune system. SNRPB plays an essential role in the splicing of pre-mRNA by binding to it and facilitating its processing into mature mRNA.
Biological function summary

SNRPB functions as part of the spliceosome complex which is responsible for removing introns from pre-mRNA. It assists in assembling and stabilizing the snRNPs required for correct splicing. The protein helps ensure cellular pathways operate smoothly by maintaining RNA integrity which is important for gene regulation and expression. SNRPB with other Sm proteins forms a stable core structure needed for snRNP function within the spliceosome complex.

Pathways

Scientists connect SNRPB to the mRNA splicing and gene expression pathways. In particular it interacts closely with other snRNPs like SNRPD1 and SNRPD2 which are also integral to mRNA processing. The accurate function of these proteins within the pathway ensures that cells produce correct protein variants which are essential for cell survival and adaptation to environmental changes.

Researchers often associate SNRPB with spinal muscular atrophy (SMA) and systemic lupus erythematosus (SLE). In SMA dysfunction in snRNP assembly due to mutations in related proteins like SMN1 can lead to motor neuron degeneration. In SLE autoimmune reactions often target snRNPs including SNRPB leading to chronic inflammation and tissue damage. Understanding these associations helps in the exploration of potential therapeutic targets for these challenging diseases.
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Specifications

Form

Liquid

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General info

Function

Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed : 11991638, PubMed : 18984161, PubMed : 19325628, PubMed : 25555158, PubMed : 26912367, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166, PubMed : 32494006). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077). As part of the U7 snRNP it is involved in histone pre-mRNA 3'-end processing (PubMed : 12975319).

Sequence similarities

Belongs to the snRNP SmB/SmN family.

Post-translational modifications

Methylated by PRMT5 (By similarity). Arg-108 and Arg-112 are dimethylated, probably to asymmetric dimethylarginine (PubMed:16087681, Ref.10).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed : 11991638, PubMed : 18984161, PubMed : 19325628, PubMed : 25555158, PubMed : 26912367, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166, PubMed : 32494006). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077). As part of the U7 snRNP it is involved in histone pre-mRNA 3'-end processing (PubMed : 12975319).
See full target information SNRPB
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