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AB102559

Recombinant Human SNRPF protein

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Recombinant Human SNRPF protein is a Human Full Length protein, in the 1 to 86 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PBSCF, SNRPF, Small nuclear ribonucleoprotein F, snRNP-F, Sm protein F, Sm-F, SmF

1 Images
SDS-PAGE - Recombinant Human SNRPF protein (AB102559)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SNRPF protein (AB102559)

15% SDS-PAGE analysis of ab102559 (3 μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P62306

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSLPLNPKPFLNGLTGKPVMVKLKWGMEYKGYLVSVDGYMNMQLANTEEYIDGALSGHLGEVLIRCNNVLYIRGVEEEEEDGEMRE","proteinLength":"Full Length","predictedMolecularWeight":"11.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":86,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P62306","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNRPF also known as Small Nuclear Ribonucleoprotein Polypeptide F plays a mechanical role in the spliceosomal complex. It is a component of the U1 U2 U4 U5 and U6 small nuclear ribonucleoproteins (snRNPs) essential in mRNA splicing. This protein has a mass of approximately 9.5 kDa. It is mainly expressed in the nucleus of eukaryotic cells where it participates in RNA processing.
Biological function summary

SNRPF functions as a part of the spliceosome assembly a large ribonucleoprotein complex responsible for pre-mRNA splicing. This process modifies nascent RNA transcripts converting them into mature mRNA for translation. The role of SNRPF in association with other Sm core proteins enables the assembly and stabilization of the snRNPs facilitating the accurate execution of the splicing cycle.

Pathways

The involvement of SNRPF in mRNA processing stands out significantly. It acts within the spliceosome complex playing an important part in the mRNA splicing pathway. This pathway ensures the removal of introns and the proper joining of exons vital for producing functional RNA transcripts. SNRPF interacts with other proteins like SNRPD1 and SNRPD2 forming an intricate network pivotal in the regulation of gene expression.

Defects in SNRPF expression or function have links to neurodegenerative disorders and certain cancers. Misregulation of splicing events potentially caused by altered levels of SNRPF connects to conditions such as spinal muscular atrophy. The protein's interactions with other splicing factors might influence the development of these diseases highlighting the importance of its proper regulation in cellular processes.
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Specifications

Form

Liquid

Additional notes

ab102559 was purified using conventional chromatography techniques.

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General info

Function

Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed : 11991638, PubMed : 18984161, PubMed : 19325628, PubMed : 23333303, PubMed : 25555158, PubMed : 26912367, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166, PubMed : 32494006). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077). As part of the U7 snRNP it is involved in histone 3'-end processing (PubMed : 12975319).

Sequence similarities

Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed : 11991638, PubMed : 18984161, PubMed : 19325628, PubMed : 23333303, PubMed : 25555158, PubMed : 26912367, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166, PubMed : 32494006). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077). As part of the U7 snRNP it is involved in histone 3'-end processing (PubMed : 12975319).
See full target information SNRPF
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