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AB114700

Recombinant Human SOAT 1/ACAT1 protein

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Recombinant Human SOAT 1/ACAT1 protein is a Human Fragment protein, in the 249 to 321 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

ACACT, ACACT1, ACAT, ACAT1, SOAT, STAT, SOAT1, Sterol O-acyltransferase 1, Acyl-coenzyme A:cholesterol acyltransferase 1, Cholesterol acyltransferase 1, ACAT-1

1 Images
SDS-PAGE - Recombinant Human SOAT 1/ACAT1 protein (AB114700)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human SOAT 1/ACAT1 protein (AB114700)

12.5% SDS-PAGE showing ab114700 at approximately 33.66 kDa stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

P35610

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein).</p>" } } }
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Product details

This product was previously labelled as SOAT 1.
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Sequence info

[{"sequence":"PPASRFIIIFEQIRFVMKAHSFVRENVPRVLNSAKEKSSTVPIPTVNQYLYFLFAPTLIYRDSYPRNPTVRWG","proteinLength":"Fragment","predictedMolecularWeight":"33.66 kDa","actualMolecularWeight":null,"aminoAcidEnd":321,"aminoAcidStart":249,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P35610","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol (PubMed : 16154994, PubMed : 16647063, PubMed : 32433613, PubMed : 32433614, PubMed : 32944968, PubMed : 9020103). Plays a role in lipoprotein assembly and dietary cholesterol absorption (PubMed : 16154994, PubMed : 9020103). Preferentially utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) as a substrate : shows a higher activity towards an acyl-CoA substrate with a double bond at the delta-9 position (9Z) than towards saturated acyl-CoA or an unsaturated acyl-CoA with a double bond at the delta-7 (7Z) or delta-11 (11Z) positions (PubMed : 11294643, PubMed : 32433614).

Sequence similarities

Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

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Product protocols

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Target data

Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol (PubMed : 16154994, PubMed : 16647063, PubMed : 32433613, PubMed : 32433614, PubMed : 32944968, PubMed : 9020103). Plays a role in lipoprotein assembly and dietary cholesterol absorption (PubMed : 16154994, PubMed : 9020103). Preferentially utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) as a substrate : shows a higher activity towards an acyl-CoA substrate with a double bond at the delta-9 position (9Z) than towards saturated acyl-CoA or an unsaturated acyl-CoA with a double bond at the delta-7 (7Z) or delta-11 (11Z) positions (PubMed : 11294643, PubMed : 32433614).
See full target information SOAT1
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Product promise

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