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AB185836

Recombinant Human SOAT 2 protein (His tag)

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Recombinant Human SOAT 2 protein (His tag) is a Human Full Length protein, in the 1 to 397 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

ACACT2, ACAT2, SOAT2, Sterol O-acyltransferase 2, Acyl-coenzyme A:cholesterol acyltransferase 2, Cholesterol acyltransferase 2, ACAT-2

Key facts

Purity

>95% SDS-PAGE

Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, HPLC

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Complementary Products

Primary Antibodies

AB230210

Anti-SOAT 2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOAT 2 antibody (AB230210)

  • 0
  • 0
Primary Antibodies

AB242315

Anti-HMGCR antibody [CL0260]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCR antibody [CL0260] (AB242315)

  • 4
  • 3
Primary Antibodies

AB30532

Anti-LDL Receptor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody (AB30532)

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Proteins & Peptides

AB132810

Recombinant Human HMGCR protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human HMGCR protein (GST tag N-Terminus) (AB132810)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as ACAT2, Acetyl-CoA acetyltransferase
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Sequence info

[{"linker":null,"sequence":"MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSMNAGSDPVVIVSAARTIIGSFNGALAAVPVQDLGSTVIKEVLKRATVAPEDVSEVIFGHVLAAGCGQNPVRQASVGAGIPYSVPAWSCQMICGSGLKAVCLAVQSIGIGDSSIVVAGGMENMSKAPHLAYLRTGVKIGEMPLTDSILCDGLTDAFHNCHMGITAENVAKKWQVSREDQDKVAVLSQNRTENAQKAGHFDKEIVPVLVSTRRGLIEVKTDEFPRHGSNIEAMSKLKPYFLTDGTGTVTPANASGINDGAAAVVLMKKSEADKRGLTPLARIVSWSQVGVEPSIMGIGPIPAIKQAVTKAGWSLEDVDIFEINEAFAAVSAAIVKELGLNPEKVNIEGGAIALGHPLGASGCRILVTLLHTLERMGRSRGVAALCIGGGMGIAMCVQRE","proteinLength":"Full Length","predictedMolecularWeight":"59.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":397,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O75908","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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General info

Function

Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol (PubMed : 11294643, PubMed : 16647063). Plays a role in lipoprotein assembly and dietary cholesterol absorption (PubMed : 11294643). Utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) and linolenoyl-CoA ((9Z,12Z,15Z)-octadecatrienoyl-CoA) as substrates (PubMed : 11294643). May provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa (PubMed : 11294643).. Isoform 2. Has lower enzymatic activity compared to isoform 1.. Isoform 3. Has lower enzymatic activity compared to isoform 1.

Sequence similarities

Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Post-translational modifications

Polyubiquitinated by AMFR/gp78 at Cys-277, leading to its degradation when the lipid levels are low (PubMed:28604676). Association with AMFR/gp78 is mediated via interaction with INSIG1 (PubMed:28604676). High concentration of cholesterol and fatty acid results in Cys-277 oxidation, preventing ubiquitination at the same site, resulting in protein stabilization (PubMed:28604676).. Oxidized at Cys-277: high concentration of cholesterol and fatty acid induce reactive oxygen species, which oxidizes Cys-277, preventing ubiquitination at the same site, and resulting in protein stabilization.

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Product protocols

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Target data

Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol (PubMed : 11294643, PubMed : 16647063). Plays a role in lipoprotein assembly and dietary cholesterol absorption (PubMed : 11294643). Utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) and linolenoyl-CoA ((9Z,12Z,15Z)-octadecatrienoyl-CoA) as substrates (PubMed : 11294643). May provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa (PubMed : 11294643).. Isoform 2. Has lower enzymatic activity compared to isoform 1.. Isoform 3. Has lower enzymatic activity compared to isoform 1.
See full target information SOAT2
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