Recombinant human SOD2/MnSOD protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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- Cell death and differentiation 29:1769-17892022A novel HIF-2α targeted inhibitor suppresses hypoxia-induced breast cancer stemness via SOD2-mtROS-PDI/GPR78-UPR axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanyuan Yan,Miao He,Lin Zhao,Huizhe Wu,Yanyun Zhao,Li Han,Binbin Wei,Dongman Ye,Xuemei Lv,Yan Wang,Weifan Yao,Haishan Zhao,Bo Chen,Zining Jin,Jian Wen,Yan Zhu,Tao Yu,Feng Jin,Minjie WeiPubMed 35301432
- Free radical biology & medicine 108:595-6092017Differential protein acetylation assists import of excess SOD2 into mitochondria and mediates SOD2 aggregation associated with cardiac hypertrophy in the murine SOD2-tg heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiwen Zhang et. al.PubMed 28433661
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, MS
- Biologically active
- Yes
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M K H S L P D L P Y D Y G A L E P H I N A Q I M Q L H H S K H H A A Y V N N L N V T E E K Y Q E A L A K G D V T A Q I A L Q P A L K F N G G G H I N H S I F W T N L S P N G G G E P K G E L L E A I K R D F G S F D K F K E K L T A A S V G V Q G S G W G W L G F N K E R G H L Q I A A C P N Q D P L Q G T T G L I P L L G I D V W E H A Y Y L Q Y K N V R P D Y L K A I W N V I N W E N V T E R Y M A C K K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Alternative names
SOD2
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Recombinant human SOD2/MnSOD protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, MS
- Biologically active
- Yes
- Biological activity
- Specific activity is > 1,200 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.8 at 25°C in a 1.5 ml reaction volume.
Activity Assay
- Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50mM potassium phosphate, 0.1mM ethylendiaminetetraacetic acid, 0.01mM cytochrome C, 0.05mM xanthine, 0.005 units xanthine oxidase.
- Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
- Add 50 ul of recombinant SOD protein in various concentrations (0.5 ug, 1 ug) in assay buffer.
- Mix by inversion and record the increase at A550nm for 5 minutes.
- Accession
- P04179-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M K H S L P D L P Y D Y G A L E P H I N A Q I M Q L H H S K H H A A Y V N N L N V T E E K Y Q E A L A K G D V T A Q I A L Q P A L K F N G G G H I N H S I F W T N L S P N G G G E P K G E L L E A I K R D F G S F D K F K E K L T A A S V G V Q G S G W G W L G F N K E R G H L Q I A A C P N Q D P L Q G T T G L I P L L G I D V W E H A Y Y L Q Y K N V R P D Y L K A I W N V I N W E N V T E R Y M A C K K
- Accession
- P04179
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab93946 was purified using conventional chromatography techniques.
General info
- Function
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
- Sequence similarities
Belongs to the iron/manganese superoxide dismutase family.
- Post-translational modifications
Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
- Subcellular localisation
- Mitochondrion matrix
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant human SOD2/MnSOD protein (ab93946)
15% SDS-PAGE analysis of ab93946 (3μg)
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