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AB93946

Recombinant human SOD2/MnSOD protein

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(2 Publications)

Recombinant human SOD2/MnSOD protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec, FuncS.

View Alternative Names

SOD2

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SDS-PAGE - Recombinant human SOD2/MnSOD protein (AB93946)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human SOD2/MnSOD protein (AB93946)

15% SDS-PAGE analysis of ab93946 (3μg)

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is > 1,200 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.8 at 25°C in a 1.5 ml reaction volume.

Activity Assay

  1. Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50mM potassium phosphate, 0.1mM ethylendiaminetetraacetic acid, 0.01mM cytochrome C, 0.05mM xanthine, 0.005 units xanthine oxidase.
  2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
  3. Add 50 ul of recombinant SOD protein in various concentrations (0.5 ug, 1 ug) in assay buffer.
  4. Mix by inversion and record the increase at A550nm for 5 minutes.
Accession

P04179

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGDVTAQIALQPALKFNGGGHINHSIFWTNLSPNGGGEPKGELLEAIKRDFGSFDKFKEKLTAASVGVQGSGWGWLGFNKERGHLQIAACPNQDPLQGTTGLIPLLGIDVWEHAYYLQYKNVRPDYLKAIWNVINWENVTERYMACKK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P04179","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

ab93946 was purified using conventional chromatography techniques.

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General info

Function

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

Sequence similarities

Belongs to the iron/manganese superoxide dismutase family.

Post-translational modifications

Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.. Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting (By similarity).. Polyubiquitinated; leading to proteasomal degradation. Deubiquitinated by USP36 which increases protein stability.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
See full target information Superoxide dismutase [Mn], mitochondrial
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 29:1769-1789 PubMed35301432

2022

A novel HIF-2α targeted inhibitor suppresses hypoxia-induced breast cancer stemness via SOD2-mtROS-PDI/GPR78-UPR axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Yan,Miao He,Lin Zhao,Huizhe Wu,Yanyun Zhao,Li Han,Binbin Wei,Dongman Ye,Xuemei Lv,Yan Wang,Weifan Yao,Haishan Zhao,Bo Chen,Zining Jin,Jian Wen,Yan Zhu,Tao Yu,Feng Jin,Minjie Wei

Free radical biology & medicine 108:595-609 PubMed28433661

2017

Differential protein acetylation assists import of excess SOD2 into mitochondria and mediates SOD2 aggregation associated with cardiac hypertrophy in the murine SOD2-tg heart.

Applications

Unspecified application

Species

Unspecified reactive species

Liwen Zhang,Chwen-Lih Chen,Patrick T Kang,Zhicheng Jin,Yeong-Renn Chen
View all publications
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