Recombinant human Somatostatin Receptor 2 protein - Active (His tag) is a Human Full Length protein, in the 1 to 369 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level and suitable for WB, FuncS, BL.
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Key facts
- Endotoxin level
- < 1 EU/µg
- Expression system
- Mammalian
- Tags
- His tag C-Terminus
- Applications
- WB, FuncS, BL
- Biologically active
- Yes
Amino acid sequence
M D M A D E P L N G S H T W L S I P F D L N G S V V S T N T S N Q T E P Y Y D L T S N A V L T F I Y F V V C I I G L C G N T L V I Y V I L R Y A K M K T I T N I Y I L N L A I A D E L F M L G L P F L A M Q V A L V H W P F G K A I C R V V M T V D G I N Q F T S I F C L T V M S I D R Y L A V V H P I K S A K W R R P R T A K M I T M A V W G V S L L V I L P I M I Y A G L R S N Q W G R S S C T I N W P G E S G A W Y T G F I I Y T F I L G F L V P L T I I C L C Y L F I I I K V K S S G I R V G S S K R K K S E K K V T R M V S I V V A V F I F C W L P F Y I F N V S S V S M A I S P T P A L K G M F D F V V V L T Y A N S C A N P I L Y A F L S D N F K K S F Q N V L C L V K V S G T D D G E R S D S K Q D K S R L N E T T E T Q R T L L N G D L Q T S I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application BL | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for somatostatin-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. Inhibits calcium entry by suppressing voltage-dependent calcium channels. Acts as the functionally dominant somatostatin receptor in pancreatic alpha- and beta-cells where it mediates the inhibitory effect of somatostatin-14 on hormone secretion. Inhibits cell growth through enhancement of MAPK1 and MAPK2 phosphorylation and subsequent up-regulation of CDKN1B. Stimulates neuronal migration and axon outgrowth and may participate in neuron development and maturation during brain development. Mediates negative regulation of insulin receptor signaling through PTPN6. Inactivates SSTR3 receptor function following heterodimerization.
Alternative names
Somatostatin receptor type 2, SS-2-R, SS2-R, SS2R, SST2, SRIF-1, SSTR2
Recommended products
Recombinant human Somatostatin Receptor 2 protein - Active (His tag) is a Human Full Length protein, in the 1 to 369 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level and suitable for WB, FuncS, BL.
Key facts
- Endotoxin level
- < 1 EU/µg
- Expression system
- Mammalian
- Tags
- His tag C-Terminus
- Applications
- WB, FuncS, BL
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. AB314535 at 10 μg/mL can bind an Anti-SSTR2 recombinant antibody, the EC50 is 58.13-81.28 ng/mL.
- Accession
- P30874-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.14% PBS
Sequence info
Amino acid sequence
- M D M A D E P L N G S H T W L S I P F D L N G S V V S T N T S N Q T E P Y Y D L T S N A V L T F I Y F V V C I I G L C G N T L V I Y V I L R Y A K M K T I T N I Y I L N L A I A D E L F M L G L P F L A M Q V A L V H W P F G K A I C R V V M T V D G I N Q F T S I F C L T V M S I D R Y L A V V H P I K S A K W R R P R T A K M I T M A V W G V S L L V I L P I M I Y A G L R S N Q W G R S S C T I N W P G E S G A W Y T G F I I Y T F I L G F L V P L T I I C L C Y L F I I I K V K S S G I R V G S S K R K K S E K K V T R M V S I V V A V F I F C W L P F Y I F N V S S V S M A I S P T P A L K G M F D F V V V L T Y A N S C A N P I L Y A F L S D N F K K S F Q N V L C L V K V S G T D D G E R S D S K Q D K S R L N E T T E T Q R T L L N G D L Q T S I
- Accession
- P30874
- Protein length
- Full Length
- Predicted molecular weight
- 42.5 kDa
- Amino acids
- 1 to 369
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
Purification technique - Centrifugation.
General info
- Function
Receptor for somatostatin-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. Inhibits calcium entry by suppressing voltage-dependent calcium channels. Acts as the functionally dominant somatostatin receptor in pancreatic alpha- and beta-cells where it mediates the inhibitory effect of somatostatin-14 on hormone secretion. Inhibits cell growth through enhancement of MAPK1 and MAPK2 phosphorylation and subsequent up-regulation of CDKN1B. Stimulates neuronal migration and axon outgrowth and may participate in neuron development and maturation during brain development. Mediates negative regulation of insulin receptor signaling through PTPN6. Inactivates SSTR3 receptor function following heterodimerization.
- Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
- Post-translational modifications
Phosphorylated on serine and threonine residues in response to agonist stimulation, leading to receptor desensitization and rapid internalization. Phosphorylated to a greater extent on serine than threonine residues. Threonine phosphorylation is required for arrestin binding and receptor endocytosis but is not necessary for desensitization (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C, -80°C
- Aliquoting information
- Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
- The product can be stored for up to 12 months
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The somatostatin receptor 2 (SSTR2) also known as SS2R serves as a G protein-coupled receptor with a molecular mass of approximately 40-60 kDa. SSTR2 specifically binds somatostatin a peptide hormone that regulates the endocrine system neurotransmission and cell proliferation via interaction with various somatostatin receptors. Scientists find SSTR2 expressed in high levels in the pituitary gland central nervous system gastrointestinal tract and pancreatic cells.
- Biological function summary
Somatostatin receptor 2 mediates the inhibitory effects of somatostatin on cell growth and the release of other hormones. It functions as part of the receptor complex modulating intracellular signaling pathways. SSTR2 often interacts closely with other molecules like G proteins to inhibit adenylyl cyclase reducing cyclic AMP (cAMP) levels and influencing diverse cellular responses. The receptor's ability to influence such processes emphasizes its role in maintaining cellular and systemic homeostasis.
- Pathways
Somatostatin receptor 2 plays an important role in the inhibitory signaling pathways related to growth hormone regulation and neurotransmitter release. SSTR2 actively participates in the somatostatin signaling pathway intimately interacting with proteins such as adenylate cyclase and phospholipase C. Another important pathway includes the regulation of the MAPK pathway serving to modulate cell proliferation and survival reiterating its regulatory function within endocrine signaling and cellular communication.
- Associated diseases and disorders
Somatostatin receptor 2 demonstrates significant involvement in neuroendocrine tumors and acromegaly. The overexpression of SSTR2 in neuroendocrine tumors provides a valuable target for diagnostic imaging and therapeutic interventions using somatostatin analogs. In acromegaly where excess growth hormone production occurs SSTR2's modulation of growth hormone release becomes important; it often works alongside growth hormone receptors and IGF-1 to limit abnormal growth. Therefore the receptor's targeting offers therapeutic potential and advances disease understanding.
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3 product images
Blocking - Recombinant human Somatostatin Receptor 2 protein - Active (His tag) (ab314535)
Blocking experiment on Anti-SSTR2 antibody between ab314535 and CT26/Human SSTR2 Stable Cell line by flow cytometry.
Functional Studies - Recombinant human Somatostatin Receptor 2 protein - Active (His tag) (ab314535)
Measured by its binding ability in a functional ELISA. ab314535 at 10 μg/mL can bind an Anti-SSTR2 recombinant antibody, the EC50 is 58.13-81.28 ng/mL.
Western blot - Recombinant human Somatostatin Receptor 2 protein - Active (His tag) (ab314535)
All lanes: mouse anti-6*His monoclonal antibody
All lanes: Western blot - Recombinant human Somatostatin Receptor 2 protein - Active (His tag) (ab314535)
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